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《Acta Prataculturae Sinica》 2012-01
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Optimization of a chromosome mounting technique and karyotype analysis of Thymus mongolicus

YANG Ning1,TAN Yong-xia1,2,LI Qiao-xia1,JIA Ling-yun1,CHEN Xi-lian1,LIU Xiao-rui3(1.College of Life Science,Northwest Normal University,Lanzhou 730070,China;2.College of Life Science and Technology,Lanzhou University of Technology,Lanzhou 730070,China;3.Dingxi Dry Farming Research and Extension Center,Dingxi 743000,China)  
The karyotype of Thymus mongolicus was analyzed using a chromosome mounting technique.The best sampling time was between 8:45 am and 9:15 am,the best pre-treatment was 0.04% colchicine and 0.002 mol/L 8-hydroxyquinoline mixture(1∶1) for 2-3 h,and the dyeing treatment was 1 h with modified carbolic acid-magenta.Karyotype analysis of T.mongolicus showed that the chromosome number is 24 and the karyotype formula is 2n=2x=24=20m+4sm.Ten pairs are metacentric(m) and 2 pairs are submetacentric(sm).The karyotype of T.mongolicus is 2A and the karyotype asymmetry coefficient is 59.63%.
【Key Words】： Thymus mongolicus chromosome karyotype analysis
【Fund】： 国家自然科学基金(31160087);; 甘肃省自然科学基金(0803RJ ZA038);; 甘肃省教育厅研究生导师项目(1101-06);; 甘肃省农牧厅(GYC09-10);; 西北师范大学科技与知识创新基金(NWNU-KJCXGC-03-58)资助
【CateGory Index】： S682.19
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