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《Acta Prataculturae Sinica》 2012-03
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Genetic diversity of Pisum sativum germplasm resources by ISSR

ZENG Liang1,LI Min-quan1,2,YANG Xiao-ming2(1.College of Grassland Science,Gansu Agricultural University,Lanzhou 730070,China; 2.Academy of Gansu Agriculture Science,Lanzhou 730070,China)  
The genetic diversity of the 73 materials of Pisum sativum from both at home and abroad were analyzed by ISSR marker.11 primers were selected from 100 primers which have the clearly and polymorphic bands,a total of 91 bands were obtained by 73 materials,including 78 polymorphism bands.On average,each primer amplification site for 8.2 and the polymorphism percentage was 86.4%.Shannon's index was 0.420 2 in average and the number of effective alleles was 1.451 8,the genetic similarity coefficient ranged from 0.406 5 to 0.934 0 and shows rich genetic diversity.Use groups by UPGMA cluster analysis to the genetic similarity coefficient 0.52 for boundaries,73 materials are divided into five types,clustering basic accord with geographical origin of close materials for a class together,has a certain regional distribution.
【Fund】: 现代农业食用豆产业技术体系专项(CARS-09);; 国家自然科学基金(31160304)资助
【CateGory Index】: S643.3
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