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Cloning and subcellular localization of a WRKY transcription factor gene of Medicago sativa

CHEN Ting-ting1,2,YANG Qing-chuan2,DING Wang2,KANG Jun-mei2, ZHANG Tie-jun2,ZHANG Xin-quan1(1.Department of Grassland Science,Sichuan Agricultural University,Ya'an 625014,China;2.Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China)  
The WRKY transcription factors play important roles in plant growth and development.A cDNA sequence of Medicago sativa WRKY transcription factor gene was isolated by electronic cloning and RT-PCR(reverse transcription polymerase chain reaction),and was named MsWRKY56.The cDNA sequence was 1 267 bp and contained a maximum open reading frame of 951 bp coding for 317 amino acids.Subcellular localization analysis of a construct with the transient expression vector of the MsWRKY56 gene showed that the MsWRKY56 protein was localized in the nucleus and was thus consistent with the general character of subcellular localization of transcription factors.This research forms a basis to further study the structure and function of the gene encoded protein.
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