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《Acta Prataculturae Sinica》 2012-04
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Cloning and subcellular localization of a WRKY transcription factor gene of Medicago sativa

CHEN Ting-ting1,2,YANG Qing-chuan2,DING Wang2,KANG Jun-mei2, ZHANG Tie-jun2,ZHANG Xin-quan1(1.Department of Grassland Science,Sichuan Agricultural University,Ya'an 625014,China;2.Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China)  
The WRKY transcription factors play important roles in plant growth and development.A cDNA sequence of Medicago sativa WRKY transcription factor gene was isolated by electronic cloning and RT-PCR(reverse transcription polymerase chain reaction),and was named MsWRKY56.The cDNA sequence was 1 267 bp and contained a maximum open reading frame of 951 bp coding for 317 amino acids.Subcellular localization analysis of a construct with the transient expression vector of the MsWRKY56 gene showed that the MsWRKY56 protein was localized in the nucleus and was thus consistent with the general character of subcellular localization of transcription factors.This research forms a basis to further study the structure and function of the gene encoded protein.
【Fund】: 现代农业产业技术体系建设专项资金(编号:CARS-35)资助
【CateGory Index】: S541.9
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Chinese Journal Full-text Database 4 Hits
1 ZHANG Gai-na1,2,JIA Jing-fen2(1.College of Agriculture of Henan University of Science and Technology,Luoyang 471000,China;2.School of Life Science,Northwest University,Xi'an 710069,China);Cloning of PA1 gene from Pisum sativum and genetic transformation on Medicago sativa[J];Acta Prataculturae Sinica;2009-03
2 YANG Hong-shan1,CHANG Gen-zhu1,ZHOU Xue-hui1,MIAO Xiao-lin1,LU Yuan1,WANG Yong-lin2(1.Lanzhou Institute of Animal and Veterinary Pharmaceutics Sciences,CAAS,Lanzhou 730050,China;2.Testing Station for Gangu County,Tianshui Agricultural Institute of Gansu Province,Tianshui 741200,China);A study on the introduction and cultivation of American alfalfa in semi-humid area[J];Acta Prataculturae Sinica;2010-01
3 XING Hao-ran,LIU Li-juan,LIU Guo-zhen(College of Life Sciences,Hebei Agricultural University,Baoding 071001,China);Advancement of Protein Subcellular Localization in Plants[J];Acta Agriculturae Boreali-Sinica;2006-S2
4 TIAN Yun, LU Xiang-Yang, PENG Li-Sha, FANG Jun (Biochemistry & Fermentation Lab of Life Science, Hunan Agricultural University, Changsha 410128, China);The Structure and Function of Plant WRKY Transcription Factors[J];Hereditas;2006-12
Chinese Journal Full-text Database 10 Hits
1 DOU Cai-hong et al (College of Forestry,Henan University of Science and Technology,Luoyang,Henan 471003);Construction on Specific dsRNA Interference Vector of OsWRKY10 Gene[J];Journal of Anhui Agricultural Sciences;2008-07
2 WANG Deng-zhan et al(College of Life Science,Nanjing Normal University,Nanjing,Jiangsu 210046);Cloning and Analysis of 5′ Flanking Region of Sporamin A Gene from Sweet Potato[J];Journal of Anhui Agricultural Sciences;2008-18
3 LI Chun-xi(Key laboratory of Adaptation and Evolution of Plateau Biota,Northwest Insitute of Plateau Biology, Chinese Academy of Sciences,Qinghai Xining 810001,China);A comparison of adaptive cultivation of eighteen alfalfa varieties in the Qinghai Plateau[J];Pratacultural Science;2011-11
4 LI Jian-wei1,2,WU Jian-ping1,ZHANG Li-ping1, WANG Xin-rong1,LUO Zhi-na1,2,CHEN Ben-jian1,2(1.Gansu Agricultural University,Gansu Lanzhou 730070,China;2.Key Laboratory of Grassland Ecosystem, Ministry of Education,Gansu Agricultural University,Gansu Lanzhou 730070,China);Effects of sowing quantity on production characteristics of Onobrychis viciaefolia and Medicago varia at alpine region in Lintan County[J];Pratacultural Science;2011-11
5 SHEN Bao-yun1,2,LIU Yu-hui 1,ZHANG Jun-lian1,2,WANG Di 1,2 (1.Gansu Key Laboratory of Crop Genetic &Germplasm Enhancement,Lanzhou 730070,China; 2.Agronomic College of Gansu Agricultural University,Lanzhou 730070,China);Analysis of cloning and sequence characteristics of cDNA encoding the GBSS Ⅰ gene from tubers of Solanum tuberosum[J];Acta Prataculturae Sinica;2010-05
6 MA Jiang-tao1,2,WANG Zong-li1,HUANG Dong-guang3,WU Yan-min2(1.Grassland Research Institute,Chinese Academy of Agricultural Sciences,Huhhot 010010,China;2.Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China;3.Shenzhen Techand Ecology & Environment Co.,Ltd.,Shenzhen 518040,China);Application of genetic engineering in forage plants breeding[J];Acta Prataculturae Sinica;2010-06
7 YI Lan-zhi,LI Chang-zhong,LIU Hong-qing,YANG Guo-feng,KONG Fan-zhen,SUN Juan(Institute of Economic Herb Plants of Qingdao Agricultural University,Qingdao 266109,China);Adaptation of different alfalfa varieties in Qingdao,Shandong province[J];Acta Prataculturae Sinica;2011-02
8 ZHANG Fang,WANG Zhou,ZONG Jun-qin,LIU Jian-xiu,SHE Jian-ming(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China);Establishment of an Agrobacterium mediated genetic transformation system for Eremochloa ophiuroides[J];Acta Prataculturae Sinica;2011-02
9 LIU Xiao-jing1,2,HAO Feng1,2,ZHANG De-gang1,2,MAO Juan3,YU Tie-feng1,2(1.College of Grassland Science,Gansu Agricultural University,Lanzhou 730070,China;2.Key Laboratory of Grassland Ecosystem of Ministry of Education,Sino-U.S.Centers for Grazing land Ecosystem Sustainability,Lanzhou 730070,China;3.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China);Construction of antifreeze gene CBF_2 expression vector and transformation into alfalfa callus[J];Acta Prataculturae Sinica;2011-02
10 JIANG Teng,LIN Yong-xiang,LIU Xue,JIANG Hai-yang,ZHU Su-wen (Anhui Province Key laboratory of Crop Biology,School of Life Science,Anhui Agricultural University,Hefei 230036,China);Genome-wide analysis of the WRKY transcription factor family in Medicago truncatula[J];Acta Prataculturae Sinica;2011-03
China Proceedings of conference Full-text Database 1 Hits
1 ZUO Qing,DUAN Yu-xi,CHEN Li-jie,ZHU Xiao-feng,WANG Yuan-yuan (Nematology Institute of Northern China,Shenyang Agricultural University, Shenyang 110866,China);Application of Protein Subcellular Localization Methods in Plant Pathology[A];[C];2011
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 KANG Ai min 1 , LONG Rui jun 2 , SHI Shang li 2 , WEI Xiao hong 3 , SUN Juan 2 (1. Animal and Veterinary Institute, Gansu Lanzhou; 2. Pratacultural college , Gansu Agricultural University, Gansu Lanzhou; 3. Agrinomy College;The Nutrition and Feeding Value of Alfalfa[J];Grassland and Turf;2002-03
2 HU Jian-zhong (College of Soil and Water Conservation, Beijing Forestry University, Beijing 100083,China);Evaluation of the adaptability of Bromus biebersteinii,a fine grass species for soil & water conservation introduced from USA to the Loess Plateau[J];Pratacultural Science;2003-08
3 YANG Xiu-juan,HAN Rui-hong,LU Xin-shi,DONG Jing-hua(1.Beijing Wanfang Data Co.,Ltd.,Beijing 100097,China;2.College of Life Science,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;3.Institute of Resources and Environment,Beijing Forestry University,Beijing 100083,China);Preliminary study on drought resistance of alfalfa seedling under water stress[J];Pratacultural Science;2008-11
4 WANG Zhan-zhe1,WANG Gang2,ZHAO Dian-chen1,LU Yong-xiang1(1.Northeast Institute of Geography and Agroecology,CAS,Harbin 150040,China;2.Heilongjiang School of Agricultural Broadcasting and TV,Harbin 150090,China);Study on productivity of Medicago sativa with different agronomic measures in black-soil region of mid-tempenate agriculture zone[J];Pratacultural Science;2008-12
5 Tao Ling (Institute of Desert Research, Chinese Academy of Sciences, Lanzhou 730000);DROUGHT-RESISTANCE TYPES OF NATIVE LUCERNE IN GANSU PROVINCE[J];PRATACULTURAL SCIENCE;1998-06
6 GUO Zhenggang,ZHANG Zihe,WANG Suomin,TIAN Fuping(College of pastoral agriculture science and technology, Lanzhou University, Lanzhou 730020, China);Study on adaptability of nine lucerne varieties in hills and valleys of loess plateau[J];Acta Pratacultural Science;2003-04
7 QI Zhi-qiang1,2,YU Yong-xiong1,HU Yue-gao2,ZENG Zhao-hai2 (1.College of Animal Science and Technology,Southwest University,Chongqing 400716,China;2.Pratacultural Engineering Research Center of Chinese Agricultural University,Beijing 100094,China);Current status and future tasks of the Medicago sativa industry in China[J];Acta Prataculturae Sinica;2008-01
8 WANG Xiao-juan1,SUN Yue-hua1,YANG Xiao-li1,ZHUO Renying2 (1.College of Pastoral Agriculture Science and Technology,Lanzhou University;Gansu Grassland Ecological Research Institute,Lanzhou 730020,China;2.The Research Institute of Subtropical of Forest,Chinese Academy of Forest,Fuyang 311400,China);Advances in construction of genetic linkage maps in Medicago sativa and their applications[J];Acta Prataculturae Sinica;2008-03
9 HU Shou-lin1,2,WAN Su-mei1,2,JIA Zhi-kuan1,WANG Yong3 (1.Research Center of Dryland Farming in Arid and Semi-arid Area,Northwest A & F University,Yangling 712100,China;2.College of Plant Sciences,Tarim University,Alar 843300,China;3.Gansu Academy of Agricultural Sciences,Lanzhou 730000,China);A study on photosynthetic characteristics of alfalfas grown for different lengths of time in the semi-humid region of the Loess Plateau[J];Acta Prataculturae Sinica;2008-05
10 GUO Jin-fang,PAN Jun-song,WANG Chen,ZHAO Zhi-yan,HE Ya-li(School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China);Research on relationships of pathogenesis-related proteins with plant disease resistance and their application in turfgrass disease-resistance breeding[J];Acta Prataculturae Sinica;2008-06
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