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《Acta Prataculturae Sinica》 2012-05
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Antioxidant activities in vitro of different polar solvent extracts of Asarum himalaicum

HE Quan-lei,MIAO Fang,LI Chun-ling(College of Life Science,Northwest A&F University,Yangling 712100,China)  
The antioxidative activities in vitro of different solvent extracts of Asarum himalaicum were studied to provide more information for future research on the medicinal value of A.himalaicum.The powdered material of A.himalaicum was extracted with 50% ethanol refluxing extraction and the ethanol condensate was evaporated to dryness under vacuum on a rotary evaporator.The condensate was then extracted with Petroleum ether,ethyl acetate or n-butanol or water at the same volume to get four different extracts.The four extracts were then examined for their antioxidative activities by DPPH(1,1-diphenyl-2-plcrylhydrazyl),Fenton,NBT(nitrotetrazolium blue chloride) reduction and Fe2+ chelation.The results suggest that the four solvent extracts of A.himalaicum had a certain degree of antioxidant activity,and the activity had a positive correlation with the concentration of the extract.The ethyl acetate extract had higher activity in scavenging DPPH·(IC50 =0.376 mg/mL) and O2-·(IC50=0.297 mg/mL Hile the n-butanol extract had the highest ·OH scavenging capacity(IC50=0.776 mg/mL).The water extract was the most powerful for chelating metal ions(IC50=0.026 mg/mL) and was more powerfull than that of BHT(2,6-di-tert-butyl-4-methylp-henol)(IC50=0.373 mg/mL).This study has shown that the ethyl acetate extract,n-butanol extract and water extract of A.himalaicum have remarkable free radical scavenging activities,and the water extract can be selected as a new metal ion chelating reagent.
【Fund】: 国家自然科学基金项目(30771454 31172365 31170366)资助
【CateGory Index】: S757
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