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Comparison and analysis of the characteristics of mutant SP2008-3 and adalayd in seashore Paspalum

CHANG Pan-pan1,2,ZHONG Xiao-xian1,LIU Zhi-wei1(1.Institute of Animal Science,Jiangsu Academy of Agricultural Science,Nanjing 210014,China; 2.Department of Grass Science,Animal Science and Technology College,Nanjing Agricultural University,Nanjing 210095,China)  
A comparison of botanical properties,of stomatal characteristics of the upper and lower leaf epidermis,and of chromosome identification and RAPD analysis between mutant SP2008-3 and Adalayd in seashore Paspalum was carried out.Stolon internode length and stem diameter were reduced by 34.77% and 11.76% in SP2008-3 compared with Adalayd and leaf length and width were increased by 22.55% and 11.11% respectively.There were no significant differences in erect stem internode length and diameter between SP2008-3 and Adalayd.In the upper leaf epidermis of SP2008-3,stomatal density was 20.58% greater,and stomatal length and width were 9.64% and 6.31% less respectivley than in Adalayd.Stomatal density in the lower leaf epidermis of SP2008-3,was 40.62% greater,and stomatal length and width were 6.73% and 5.33% less than in Adalayd.The somatic chromosome number was 2n=20 in both SP2008-3 and Adalayd.RAPD analysis showed a difference in the DNA: a 1 100 bp band was missing in SP2008-3 when the DNA was amplified by primer S369.
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