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《Acta Prataculturae Sinica》 2013-02
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Physiological responses of white clover by different leaf types associated with anti-oxidative enzyme protection and osmotic adjustment under drought stress

LI Zhou,PENG Yan,SU Xing-yuan(College of Animal Science and Technology,Sichuan Agricultural University,Ya'an 625014,China)  
Mechanisms of drought tolerance were studied in large-leaved and small-leaved white clovers by measuring physiological changes,including relative water content,electrolyte leakage,malondialdehyde(MDA),superoxide dismutase(SOD),catalase(CAT),ornithine aminotransferase(OAT) and osmotic adjustment substances(soluble sugar,free proline and betaine),under drought stress and rewatering.With an extension of drought stress,relative water content of the two materials decreased,while electrolyte leakage and MDA content increased.At the same time,SOD and CAT activities initially increased but then decreased and there was a sharp rise in OAT activity and in content of osmotic adjustment substances.Under drought stress,significantly higher relative water content,cell membrane stability,content of osmotic adjustment substances and activities of SOD,CAT and OAT as well as lower lipid peroxidation level were found in the small-leaved clover leaves compared with those of the large-leaved clover.After re-watering,there were varying degrees of recovery in the two leaf types of white clover,but overall the small-leaved white clover recovered better.
【Fund】: “十二五”农村领域国家科技计划课题(2011BAD17B03);; 教育部“春晖计划”(Z2010090)资助
【CateGory Index】: S816
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