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《Acta Prataculturae Sinica》 2014-01
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Effect of photoperiod on phytochromes and endogenous hormones of alfalfa with different fall-dormancies

FAN Wen-na;SUN Xiao-ge;NI Jun-xia;DU Hong-qi;SHI Ying-hua;YAN Xue-bing;WANG Cheng-zhang;College of Animal Science and Veterinary Medicine,Henan Agricultural University;  
To explore the effects of photoperiod on phytochromes and endogenous hormones of different fall-dormancy alfalfas( Medicago sativa),and to reveal the regulatory mechanisms,the expressions of PHYA( phytochrome A) and PHYB( phytochrome B) were observed by the FQ-PCR SYBR-Green I method after 35 days in three standard varieties: the fall-dormant variety Norseman( FD 1),the semi-dormant variety Dupuils( FD 5) and the non-dormant variety CUF101( FD 9),under different photoperiods( 7,10,13 and 16 h/d). Their endogenous hormone contents( GA 3,ZR,IAA and ABA) were measured by Enzyme-linked immunosorbent assay( ELISA). The mRNA expressions of PHYA and PHYB in the three alfalfa varieties increased gradually with a shortening photoperiod,especially in the fall-dormant variety Norseman. The ratios of GA 3 / ABA,ZR / ABA and IAA / ABA decreased with increased photoperiod and those of the Norseman variety were the lowest: it had the slowest growth,the highest ABA level and the lowest GA 3 level. It is possible that PHYA and PHYB directly or indirectly influenced the synthesis of GA 3,ZR,IAA,ABA and further regulated fall dormancy.
【Fund】: 国家自然科学基金(31172261);; 现代农业产业技术体系项目(CARS-35);; 国家科技支撑计划项目(2011BAD17B04)资助
【CateGory Index】: S541.9
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