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《Acta Prataculturae Sinica》 2014-04
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Constructing pre-core collection of Cynodon dactylon based on phenotypic data

ZHENG Yi-qi;GUO Yan;FANG Shu-juan;XU Ya-nan;CHEN Jing-bo;LIU Jian-xiu;Forestry College of Henan University of Science and Technology;Institute of Botany,Jiangsu Province and Chinese Academy of Sciences;  
In this study,the method of constructing Cynodon dactylon pre-core collection was studied based on15 quantitative and qualitative traits from 831 accessions.An optimal sampling scheme was selected from 48 strategies which consisted of sampling proportions(5%,10%,15%,20%,25%,and 30%),sampling proportions within group(simple proportion,logarithmic proportion,square root proportion and genetic proportion)and individual selection methods within group(random selection and cluster selection)evaluating with four effective parameters including mean difference percentage,variance difference percentage,coincidence rate of range and changeable rate of variation coefficient.The optimal sampling scheme was also confirmed by comparing the accessions distribution plotted by first two principal components,correlation coefficients of traits between two collections.The result indicated that 25% was the optimum sampling proportions.The square root proportion was better than simple proportion,logarithmic proportion and genetic proportion.The cluster selection was more suitable than random selection.At 25%sampling proportion,using square root proportion within group and cluster selection method within group is the most suitable method for constructing C.dactylon pre-core collection.The result conducted by PCA analysis showed the genetic structure and variation of 831 accessions was well represented by the pre-core collection.The most important phenotypic correlations between traits which may be under the control of co-adapted gene complexes were also preserved by the pre-core collection.Based on the optimized sampling strategy,the pre-core collection consisting 208 accessions of C.dactylon were established and will play an important role in the utilization of C.dactylongenetic resources.
【Fund】: 国家自然科学基金(31101754 31101567)资助
【CateGory Index】: S688.4
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