Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Prataculturae Sinica》 2014-04
Add to Favorite Get Latest Update

Cloning and expression analysis of two WRKY transcription factors from the rare recretohalophyte Reaumuria trigyna

WANG Jia;ZHENG Lin-lin;GU Tian-pei;WANG Xue-feng;WANG Ying-chun;College of Life Science,Inner Mongolia University;Inner Mongolia and Monsod Drought Resistance Greening Corporation Limited;  
Reaumuria trigynais an endangered small shrub thriving in vast arid areas of Inner Mongolia.This species has developed a distinct morphology and physiology to adapt to the semi-desert environment.The WRKY gene family are plant-specific transcription factors which play important roles in the regulation of plant development,metabolism,and diverse biotic and abiotic stresses.Based on R.trigynatranscriptome data,two cDNA fragments(annotated as WRKY transcription factors)which were significantly upregulated after salt treatment were selected.Using rapid amplification of cDNA ends(RACE),these two WRKY transcription factors were cloned from R.trigyna.Blasted in the NCBI database,the homology to Arabidopsis AtWRKY33 were 79% and 87%,so they were named RtWRKY33-1and RtWRKY33-2(GenBank accession number KF421158 and KF421159),respectively.The full-length of the RtWRKY33-1was 2163 bp and included a 1681 bp open reading frame(ORF)which encoded 573 amino acids.The full-length of RtWRKY33-2was 2155 bp and included a 1776 bp ORF encoding 591 amino acids.Amino acid sequence analysis indicated that they were typical of class I WRKY transcription factors.Protein structure prediction analysis found that the primary structure and secondary structure in the WRKY domain of the amino acid sequence had structures characteristic of high similarity.However,in the non-conserved domains,especially the N-terminus(1to 80 aa range),before the first WRKY domain(190to 240 aa range)and between two WRKY domains(430to 450 aa range),there were significant differences that may influence the function of these two genes.Semi-quantitative RTPCR analysis showed that four abiotic stresses can induce the expression of these two genes.However,RtWRKY33-2was mainly inducted by salt,cold and ABA,while RtWRKY33-2responded faster to drought stress.These two genes in R.trigyna may play a different rolein abiotic stress responses.This research is a basis for study on the regulation mechanism of WRKY33 transcription factors in R.trigyna.
【Fund】: 国家自然科学基金项目(31360063);; 内蒙古自然科学基金重大项目(2012ZD05);; 内蒙古自治区高等学校创新团队发展计划(NMGIRT1401)资助
【CateGory Index】: Q943.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 5 Hits
1 JIANG Teng,LIN Yong-xiang,LIU Xue,JIANG Hai-yang,ZHU Su-wen (Anhui Province Key laboratory of Crop Biology,School of Life Science,Anhui Agricultural University,Hefei 230036,China);Genome-wide analysis of the WRKY transcription factor family in Medicago truncatula[J];Acta Prataculturae Sinica;2011-03
2 CHEN Ting-ting1,2,YANG Qing-chuan2,DING Wang2,KANG Jun-mei2, ZHANG Tie-jun2,ZHANG Xin-quan1(1.Department of Grassland Science,Sichuan Agricultural University,Ya'an 625014,China;2.Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China);Cloning and subcellular localization of a WRKY transcription factor gene of Medicago sativa[J];Acta Prataculturae Sinica;2012-04
3 TAO Fei1,2,MA Jiang-tao2,TANG Yi-xiong2,LI Jing-sheng3, LU Yun-ming3,XU Bing-liang1,WU Yan-min2 (1.College of Grassland Science,Gansu Agricultural University,Key Laboratory of Grassland Ecosystem, Ministry of Education,Pratacultural Engineering Laboratory of Gansu Province,Sino-U.S. Centers for Grazingland Ecosystem Sustainability,Lanzhou 730070,China; 2.Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China;3.Shenzhen Nongke Group, Shenzhen 518040,China);Functional analysis of drought-resistant transcription factors PeDREB2a and HhERF2 in Lotus corniculatus[J];Acta Prataculturae Sinica;2013-03
4 ZHENG Lin-lin,ZHANG Hui-rong,HE Long-mei,WANG Ying-chun(College of Life Science,Inner Mongolia University,Key Laboratory of Herbage & Endemic Crop Biotechnology in Inner Mongolia,Hohhot 010021,China);Isolation and expression analysis of a plasma membrane Na+/H+ antiporter from Nitraria tangutorum[J];Acta Prataculturae Sinica;2013-04
5 ZHANG Le-xin1,2,SU Man1,MA Tian1,3,MA Xing-yong1,YAN Xue-qing1,2, PENG Xian-jun1,CHEN Shuang-yan1,CHENG Li-qin1,LIU Gong-she1(1.Key Laboratory of Plant Resources,Institute of Botany,Chinese Academy of Sciences, Beijing 100093,China;2.Graduate University of Chinese Academy of Sciences,Beijing 100049,China;3.Agricultural College,Ningxia University,Yinchuan 750021,China);Cloning and analysis of the Δ1-pyrroline-5-carboxylate synthetase(LcP5CS1)from Leymus chinensis[J];Acta Prataculturae Sinica;2013-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 FANG Yong-feng1,2,3,LI Yong-sheng3,BAI Jiang-ping1,2,3,MU Ping3,MENG Ya-xiong1,2,3, ZHANG Jin-lin4,WANG Han-ning1,2,3,SHANG Xun-wu3(1.Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement,Lanzhou 730070,China; 2.Gansu Provincial Key Laboratory of Aridland Crop Science,Lanzhou 730070,China; 3.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China; 4.College of Pastoral Agriculture Science and Technology, Lanzhou University,Lanzhou 730020,China);Construction of integration QTL map and identification of candidate genes for stay-green in maize[J];Acta Prataculturae Sinica;2012-04
2 SHI Juan1,2,WANG Hua-rong1,ZHONG Shao-lin1(1.School of Agriculture,Ningxia University,Yinchuan 750021,China;2.School of Plant Protection, Northwest A & F University,Yinchuan 750021,China);Ultrastructural characteristics of compatible Pseudopeziza medicaginis interaction with alfalfa leaf[J];Acta Prataculturae Sinica;2012-05
3 CHEN Ting-ting1,2,YANG Qing-chuan1,ZHANG Xin-quan2,KANG Jun-mei1, DING Wang1,ZHANG Tie-jun1(1.Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China; 2.Department of Grassland Science,Sichuan Agricultural University,Ya'an 625014,China);Bioinformatics and expression analyses of ethylene response factor genes in Medicago[J];Acta Prataculturae Sinica;2012-06
4 LI Yan1,SUN Yan2,YANG Qing-chuan1,KANG Jun-mei1,ZHANG Tie-jun1,FANG Feng3(1.Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China; 2.College of Animal Science and Technology,China Agriculture University,Beijing 100191, China;3.Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China);Construction and transformation of an over-expression plasmid of the MsZIP gene from Medicago sativa[J];Acta Prataculturae Sinica;2012-06
5 YANG Hong-shan1,CHANG Gen-zhu1,BAO Wen-sheng2(1.Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS,Lanzhou 730050,China; 2.Space Breeding Engineering Research Center of Gansu Province,Tianshui 741030,China);Preliminary study on the space mutation breeding of alfalfa[J];Pratacultural Science;2013-02
6 TAO Fei1,2,MA Jiang-tao2,TANG Yi-xiong2,LI Jing-sheng3, LU Yun-ming3,XU Bing-liang1,WU Yan-min2 (1.College of Grassland Science,Gansu Agricultural University,Key Laboratory of Grassland Ecosystem, Ministry of Education,Pratacultural Engineering Laboratory of Gansu Province,Sino-U.S. Centers for Grazingland Ecosystem Sustainability,Lanzhou 730070,China; 2.Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China;3.Shenzhen Nongke Group, Shenzhen 518040,China);Functional analysis of drought-resistant transcription factors PeDREB2a and HhERF2 in Lotus corniculatus[J];Acta Prataculturae Sinica;2013-03
7 WANG Li;ZHANG Jun-lian;ZHANG Jin-wen;LIU Yu-hui;Bai Jiang-ping;YU Bin;YANG Hong-yu;WANG Di;Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement;College of Life Sciences and Technology,Gansu Agricultural University;Gansu Provincial Key Lab of Aridland Crop Science;College of Agronomic,Gansu Agricultural University;;Cloning and analysis of sequence characteristics of cDNA encoding the AtHKT1 gene from Arabidopsis thaliana leaves[J];Acta Prataculturae Sinica;2013-06
8 JIN Juan;LIANG Jin;HE Chun-gui;College of Pratacultural Science,Gansu Agricultural University;Key Laboratory of Ecosystem Ministry of Education,Sino US Center for Grazingland Ecosystem Sustainability;Gansu Provincial Academy of Agricultural Sciences;;Antibiosis of five alfalfa cultivars to two colour morphs of Acyrthosiphon pisum[J];Acta Prataculturae Sinica;2013-06
9 FAN Wen-na;SUN Xiao-ge;NI Jun-xia;DU Hong-qi;SHI Ying-hua;YAN Xue-bing;WANG Cheng-zhang;College of Animal Science and Veterinary Medicine,Henan Agricultural University;;Effect of photoperiod on phytochromes and endogenous hormones of alfalfa with different fall-dormancies[J];Acta Prataculturae Sinica;2014-01
10 CUI Tong-xia;BAI Jiang-ping;WEI Gui-ming;ZHAO Xu;WANG Di;ZHANG Jin-wen;Gansu Key Laboratory of Crop Improvement & Germplasm Enhancement,Gansu Provincial Key Lab of Aridland Crop Science,Agronomy Faculty,Gansu Agricultural University;Gansu State Farms Academy of Agricultural Researches;;Red light induced SGT3 gene expression and functional analysis of the SGT3 promoter in potato[J];Acta Prataculturae Sinica;2014-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHAO Xiang-Qiang(School of Life Sciences,Nantong University,Nantong,Jiangsu 226007);Cloning and Identification of A New Na~+/H~+ Antiporter Gene ZmSOS1 in Maize[J];Journal of Anhui Agricultural Sciences;2009-35
2 YANG Hai-yan et al(Department of Chemistry and Life Science,Chuxiong Normal University,Chuxiong,Yunnan 675000);Effect of Drought Stress on Physiological Index of Fern[J];Journal of Anhui Agricultural Sciences;2011-11
3 LU Hai~1, ZHANG Jianqiu~1, ZHANG Yuling~1, GOU Xiaojun~2 (1.College of Biological Science and Biotechnology, Beijing Forestry University, Beijing 100830, China; 2.Lab for Molecular Evolution Engineering, Chengdu University, Chengdu 610106, China);An Improved Method of mRNA Differential Display for Salt-Base Induced Gene Expression of N.Sibirica Pall[J];Journal of Chengdu University (Natural Science);2004-02
4 Wang Xue min,Yi Jin (Inner Mongolia Agricultural University, Huhhot, Inner Mongolia Autonomous Region 010018, China);The Study on Germinating Conditions and Physiological Characteristics of Ceratoides Seeds[J];Acta Agrestia Sinica;2003-02
5 ShenYuing Yan ShunGuo Yu Ling (Gansu Grassland Ecological Research Institute,Lanzhou 730020);EFFECTS OF SALT CAACENTRATIONS ON THE SEED GERMINATION OF KAREANALKALGEAES[J];Pratacultural Science;1991-03
6 FENG Yu-qin1,CAO Zhi-zhong2(1.Institute of Crop Product Processing and Storage,Gansu Academy of Agriculture Science,Lanzhou 730070,China;2.College of Pratacultural Science,Gansu Agricultural University,Lanzhou 730070,China);Study on the nutrition quality of wild germplasm of Medicago lupulina[J];Pratacultural Science;2009-10
7 LI Long-rui 1,2,ZHANG Ji-kun 2,3,ZOU Qing-hua 3(1.Veterinary College,South China Agricultural University,Guangzhou 510642,China;2.Veterinary Medicine Research Institute,Jiangxi New World Pharmaceutical Co Ltd,Xiajiang 331400,China;3.Jiangxi Academy of Agricultural Science,Nanchang 330200,China);Effects of addition of Lotus corniculatuinto rice straw on methane emission in vitro[J];Pratacultural Science;2012-01
8 WANG Sheng-yin,MA Qing,WANG Suo-min(State Key Laboratory of Grassland Agro-ecosystems;College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China);Cloning and sequence analysis of a plasma membrane Na~+/H~+ antiporter fragment from halophyte Suaeda salsa[J];Pratacultural Science;2012-06
9 Liang Yunmei (Normal University of Eastern China, Shanghai 200062) Li Yan (No. 90 Middle School of Tianjin City, Tianjin 300070) Duo Li’an (Tianjin Normal University, Tianjin 300074) Liu Jie (Bureau of Agriculture and Forestry of Tianjin City, T;EFFECT OF SALT STRESS ON GERMINATION OF LUCERNE SEEDS[J];PRATACULTURAL SCIENCE;1998-06
10 Wang Suomin Zhu Xingyun Wang Zengrong(Gansu Grassland Ecological Research Institute, Lanzhou 730020);A Preliminary Study on the Effects of Osmotic Adjustment on Alkaligrass (Puccinellia tenuiflora ) Seedling Growth under Salt Stress[J];Acta Prataculturae Sinica;1993-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved