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The transferability of SSR and EST-SSR markers of different origins in Elymus and Roegneria in the Triticeae(Poaceae)

CHEN Shi-Yong;MA Xiao;ZHANG Xin-Quan;CHEN Zhi-Hua;ZHOU Kai;Department of Grassland Science,Sichuan Agricultural University;College of Life Science and Technology,Southwest University for Nationalities;  
Elymus and Roegneria,two important genera in the tribe Triticeae,include many important forage grasses,and also carry elite genes for improving cereal crops.However,there is ongoing dispute on the species boundaries and interspecific systematic relationships in the two genera.The biosystematic relationships in the group are of keen interest to agrostologists around the world.Based on known SSR markers in Triticeae,the objective of this study was to screen out the high transferability markers for Elymus and Roegneria,which would provide important information for understanding the biosystematic relationships of the two genera.A total of 230 simple sequence repeats(SSR)and SSR based on expressed sequence tags(EST-SSR)markers from5 different genera including wheat,barley,Elymus,Pseudoroegneria and Leymus,were used to study the transferability to 23 species containing the St,H,and Y genomes.Among the 230 SSR markers,163(70.87%)markers could generate clear bands,which showed a high transferability for those markers.The EST-SSR markers(87.60%)showed a higher transferability than genomic SSR markers(49.50%),but the genomic SSR markers showed a higher polymorphism(85.98%)than the EST-SSR markers(79.37%).A total of 579 bands were amplified from the 163 SSR markers able to generate clear bands,of which 533 bands were polymorphic.The number of amplified bands of each of these 163 SSR markers ranged from 1to 11 with an average of 3.55 bands.Cluster analysis using the unweighted pair group method with arithmetic average(UPGMA)showed that the species with same or similar genome could be grouped together.Additionally,the H genome showed a rather distant phylogenetic relationship with St genome,and distant phylogenetic relationships were also revealed between the diploid species containing the H genome and other species in the study.The selected SSR markers from 5genera could be amplified successfully in Elymus and Roegneria.To summarise,a total of 163 high transferability SSR markers were found to be suitable for the further phylogeny analysis,which also revealed the phylogenetic relationships among the species.The results from this study provide important information for improving understanding of biosystematic relationships between Elymus and Roegneria.
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