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《Acta Prataculturae Sinica》 2016-12
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Effects of NO_3~--N/NH_4~+-N ratio on the nutritional quality and feeding values of alfalfa

YU Tie-Feng;LIU Xiao-Jing;HAO Feng;FAN Jun-Jun;College of Grassland Science,Gansu Agricultural University,Key Laboratory of Pratacultural Ecosystem,Ministry of Education,Pratacultural Engineering Laboratory of Gansu Province,Sino-US Center for Grazingland Ecosystem Sustainability;  
The effects of different ratios of NO_3~--N/NH_4~+-N(1/7,1/3,3/5,5/5,5/3,3/1,7/1)on alfalfa(Medicago sativa cv.Gannong No.3)in terms of its nutritional quality and feeding values were studied in an outdoor rain-protected chamber.Alfalfa plants were cultivated in sand with an optimum nitrogen supply(210mg/L).The results indicated that under the ratio of NO_3~--N/NH_4~+-N(5/3),the contents of crude protein(CP),total protein(TP),digestible dry matter(DDM)and dry matter intake(DMI)were significantly higher than their respective values in the other treatments(P0.05),while the contents of neutral detergent fiber(NDF)and acid detergent fiber(ADF)were significantly lower than their respective values in the other treatments(P0.05).Thus,the 5/3ratio was determined to be the best ratio for nutrient accumulation.From the perspective of microelement nutrition analysis,mixed nitrogen mainly with NH+4-N was more beneficial for the accumulation of iron,manganese,and molybdenum,and the adaptation ratios were 1/3,1/7,and 3/5,respectively.Mixed nitrogen mainly with NO-3-N was more beneficial for the accumulation of zinc,and the adaptation ratio was 3/1.In the relative feeding values analysis,the RFV value in the 5/3treatment was significantly higher than those in the other treatments(P0.05),and the RFV of all treatments was greater than 100.Referring to the classification standard of alfalfa grass products in America,the nutritional quality in all treatments was at least level 2,with that in the 5/3and 7/1treatments reaching level 1.When alfalfa was grown in an environment of available nitrogen mainly with NO-3-N and the ratio of NO-3-N/NH+4-N was closer to 5/3,the alfalfa crop had good nutritional quality.
【Fund】: 国家自然科学基金项目(31460622);; 公益性行业(农业)科研专项(201403048-8);; 甘肃省科技支撑项目(1504NKCA003)资助
【CateGory Index】: S541.9;S816
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