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《Journal of Changzhi Medical College》 2009-03
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Separation and Culture of Human Colorectal Cancer Cells in Vitro

Zhang Yiqiang,Cheng Niuliang Department of Biochemistry and Molelular Biology,Changzhi Medical College  
Objective:To investigate methods of separation and culture of human colorectal cancer cells in vitro.Methods: Human colorectal cancer cells are cultured by explant method and Ⅳ collagenase digestion;HE stain,Immunocytochemistry identify tissue original,and transmission electron microscope TEM were used to view cells morphous;MTT assay was usedto draw cell growth curve.Results: HE staining showed karyomegaly and blue-stained colorectal cancer cells.Immunocytochemistry staining showed(cytoderatin)CK was positive,cytoplasm was yellow;TEM showed big cell nuclear,abundance in mitochondria,cell surface microvillus and cell-cell junctional complex;Cell growth curve showed sigmoid curve.Conclusion: Explant method has a high achievement ratio.Explant should be kept asepsis during drawing materials and isolation.Cultured cells shluld be identified as colorectal cancer cells initially.
【CateGory Index】: R735.34
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