Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Dalian University》 2002-04
Add to Favorite Get Latest Update

Study on extracting and fractionating of protein from chiorella spp.

LIN Peng, LIU Van, YANG Hai-bo(College of Bioengineering, Dalian University, Dalian 116622, China; Department of Chemistry and Chemical Engineering, Dalian University, Dalian 116622, China)  
Chiorella spp. was cultivated for 8 days in at room temperature under fluorescent light. After 6 days, cells were collected by centrifugation, then disrupted by ultrasonic wave. After centrifugation, the supernatant was treated with 30% (NH4)2SO4 for 24 hour at 4℃. Again after centrifugation, the sediment and supernatant were dialyzed at 4℃ in distilled water for 24 hour. After desalination, each fraction was applied to Sephdex G-75 molecular sieve column, finally four proteins were obtained. The result was shown by PAGE.
【Fund】: 辽宁省科学技术基金资助项目(001059)
【CateGory Index】: Q946.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 7 Hits
1 YANG Hai-bo,YU Yuan,LIU Yan,ZHANG Xin-hua,LI Ying-min,and LU Fu-rong (Department of Chemistry and Chemical Engineering,Dalian University,Dalian,116622 China);Effects of Nutrients on Growth and Protein and Saccharide Contents in Chlorella vulgaris[J];Fisheries Science;2004-01
2 YU Yuan, LIU Yan, YANG Hai-bo, ZHANG Xin-hua, LI Ying-min, LU Fu-Rong, LIN Peng(Department of Chemistry and Chemical Engineering, Dalian University, Dalian 116622, China; College of Bioengineering, Dalian University, Dalian 116622, China);The effects of nutritions on the C. vulgaris growth and the content of protein[J];Journal of Dalian University;2002-06
3 TANG Hai-shu,NING Xue-fei,CHEN Liang-liang,YIN Xiao-li,LUO Jing-jing,MA Xiao-jin,SUN Su-rong(Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830046,China);Preliminary Study of the Activities of the Crude Lectin from Peganum harmala Seeds[J];Acta Botanica Boreali-Occidentalia Sinica;2010-04
4 CHEN Xiao-qing,ZHENG Yi,LIN Xiong-ping(Bioengineering College,Fujian Normal University ,Fuzhou 350007,China);Antimicrobial Activities of the Polysaccharide andProtein Extracts from Two Species of Microalgae[J];Journal of Fujian Normal University(Natural Science);2005-02
5 WEI Wen-zhi1,2,XIA Wen-shui1,WU Yu-juan2(1.School of Food Science and Technology,Southern Yangtze University,Wuxi 214036,China;2.College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China);Isolation,purification and property of anti-oxidation of the proteins from Chlorella pyrenoidosa[J];Journal of Shanghai Fisheries University;2007-02
6 WU Hai-yue1,ZHANG Fu-xin1,ZAN Lin-sen2,LI Chuan-yang1(1 College of Food Engineering and Nutritional Science,Shaanxi Normal University, Xi′an 710062,Shaanxi,China;2 College of Animal Science,Northwest Science Technology University of Agriculture and Frestry,Yangling 712100,Shaanxi,China);Influences of different salting-out reagents on effect of kid rennet[J];Journal of Shaanxi Normal University(Natural Science Edition);2008-04
7 NING Xue-fei,CHEN Liang-liang,TANG Hai-shu,SUN Su-rong Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830046,China;Distribution,Extraction and Activities of the Proteins from Peganum harmala L.[J];Natural Product Research and Development;2009-05
【Citations】
Chinese Journal Full-text Database 2 Hits
1 CHEN Xing-cai,LI Xiang,LIU Xing-zhu,LIN Ping (College of Qiao Xing Light Industry,Fuzhou University,Fujian Fuzhou 350002,China);Study on Extracting Process of Protein from Spirulina Platensis[J];JOURNAL OF FUZHOU UNIVERSITY(NATURAL SCIENCES EDTION);1999-06
2 WANG Yong,QIAN Kai Xian (Department of Biological Science and Technology,Zhejiang University,Hangzhou 310027,China);DONG Qiang (Institute of Medical Science,Jinan 250062,China).;The Study for Isolation and Purification of Phycocyanin with High Purity and Its Spectra Characteristics .[J];PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS;1999-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Hui et al (Soil & Environment College,Shenyang Agricultural University,Shenyang,Liaoning 110161);Study and Exploration of Phycobiliprotein of Spirulina[J];Journal of Anhui Agricultural Sciences;2006-21
2 LIU Jie et al(Marine College,Shandong University at Weihai,Weihai,Shandong 264209);Research Advance in Tissue Culture Technology of Marine Macroalgae[J];Journal of Anhui Agricultural Sciences;2009-19
3 JIANG Huai-zhen et al(College of Food Science and Engineering,China Ocean University,Qingdao,Shandong 266003);Research on the Protein Extracted from Chlorella with the Method of Thermal Steam-NaOH[J];Journal of Anhui Agricultural Sciences;2009-33
4 CAI Jin-zhong et al(Laboratory of Food and Drug Safety Test,Xiamen Huaxia Vocational College,Xiamen,Fujian 361024);Research on the Fluorescence Characteristics of Synechococcus sp.and Salt Algae[J];Journal of Anhui Agricultural Sciences;2010-17
5 LI Hui et al (Institute of Biological and Pharmaceutical Engineering,Nanjing Industry University,Nanjing,Jiangsu 210009);Chlorella Resistance to Dehydration Conditions and Protection Effect of Different Cryoprotecants on Chlorella Cells[J];Journal of Anhui Agricultural Sciences;2011-01
6 GONG Dong-hui1,2,ZHANG Shao-ying1,QIAO Chen1,LIU Hua3(1.Agricultural College,Inner Mongolia Agricultural University,Huhhot 010019; 2.Institute of Bioengineering and Technology in IMUST,UST Inner Mongolia,Baotou 014010;3.School of Forestry & Landscape Architecture,Anhui Agricultural University,Hefei 230036);Daily variation of photosynthetic rate and respiratory rate of Spirulina (Arthrospira) in greenhouse in summer[J];Journal of Anhui Agricultural University;2010-04
7 JIN Hai-long,WANG Yu-tian (Engineering of Electric Institute of YanShan University, Qinhuangdao Hebei 066004,China);Research on Recognition Method of Living Alga Based on Stimulating Fluorescence Spectrum[J];Chinese Journal of Sensors and Actuators;2006-01
8 JIN Hai-long~,WANG Yu-tian (Electronics institute of YanShan University , Qinhuangdao HeBei 066004,China);Hybrid Alga Identify Method Research Based on Fluorescence and Analysis of Multi-Element Regression Analysis[J];Chinese Journal of Sensors and Actuators;2006-04
9 YANG Hai bo,YU Yuan,ZHANG Xin hua,LI Ying min (Department of Chemistry and Chemical Engineering,Dalian University,Dalian 116622,China);Study on Immobilization Culture of Marine Microalga, Chlorella vulgaris[J];Fisheries Science;2001-05
10 LIN Xia,GU Xiao ying,ZHENG Zhong ming (Faculty of life Science and Biotechonlogy,Ningbo University,Ningbo 315211,China);Primary Exploration about the Prevention and Elimination of Hostile Organsims Occured in the Cultivation of Sargassum fusiforme[J];Fisheries Science;2001-06
China Proceedings of conference Full-text Database 5 Hits
1 Zhang Shao-bin, Yan-an, Shang Shu-tian, Ren dong-tao, and Liu Guo-qin (State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Science, China Agricultural University, Beijing 100094, China );Purification with High Quality, Spectrum Characterization and Crystallization of C-phycocyanin from a Direct Line Mutant of Spirulina Platensis (SP-D)[A];[C];2004
2 HU-Yucai QU-Bing (Dalian Fisheries University,Dalian,116023);The effect on photosynthesis of chlorella vulgaris by high voltage electric field[A];[C];2006
3 YANG Gui-juan,LUAN Zhong-qi (School of Science,Dalian Fisheries University,Dalian,Liaoning,116023,China);Effect of Electrostatic Field on the Oxygen-Production of Chlorella Vulgaris[A];[C];2009
4 Zhu Lin, Yin Lihong, Pu Yuepu;Primary studies on remove of nitrogen and phosphorus by co-immobilized nitrifying bacteria and chlorella system[A];[C];2005
5 LIU Ming-qiu~(1,2),YANG Xian-qing~1,QI-Bo~1,DENG Jian-chao,SONG Ying~(1,2) (1.South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences, National Research and Development Center for Aquatic Product Processing,Key Laboratory of Aquatic Product Processing,Ministry of Agriculture,Guangzhou 510300,China; 2.College of Food Science & Technology,Shanghai Ocean University,Shanghai 201306,China);Present situation and prospect of polysaccharide and phycobiliprotein from Gracilaria[A];[C];2013
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LI Yi-ming~(1),SHEN Ye-shou~(1),WANG Qing~(2),JI Jun-qiu~(2)(1.School of Life Science,Anhui University,Hefei 230039,China;2.Hefei Lifang Pharmaceutical Company limited,Hefei 230088,China);Method of screening the drug for antienoplastic[J];Journal of Anhui University(Natural Sciences);2005-01
2 LI Feng-ling et al (Rice Research Institute of Sichuan Agricultural University,Wenjiang,Sichuan 611130);Plant Lectin and its Application in the Genetic Engineering of Plant Resistance to Insects[J];Journal of Anhui Agricultural Sciences;2006-03
3 PAN Ke et al(College of Natural Resource and Environment,South-China Agricultural University,Guangzhou,Guangdong 510642);Purification of a Lectin from Alocasia macrorrhiza and its Toxic Effect on Four Cultured Insect Cell Line[J];Journal of Anhui Agricultural Sciences;2007-18
4 CHEN Jing-yi et al (State Key Laboratory of Food Science and Technology,School of Food Science and Technology,Jiangnan University,Wuxi,Jiangsu 214122);Study on Determination of Hegglutination Ativity of the Lectin from Canavalia ensiformis(L.) DC and its Characteristics[J];Journal of Anhui Agricultural Sciences;2008-01
5 WANG Qi-lin et al(College of Life Science,Liaocheng University,Liaocheng,Shandong 252059);Research Progress on the Cellular Immunity and Anti-tumor of Polysaccharide Agglutinin[J];Journal of Anhui Agricultural Sciences;2009-05
6 CHEN Yao et al(Pharmaceutical and Life Science College of Nanjing University of Technology,State Key Laboratory of Material-oriented Chemical Engineering,Nanjing,Jiangsu 210009);Effects of pH Value on Adsorption of Basic Ion Exchange Resin on Fumaric Acid[J];Journal of Anhui Agricultural Sciences;2009-15
7 SHI Xiao dong CHEN Xiu lan HE Hai lun ZHANG Yu zhong (state Key Lab of Microbial Technology,institute of Microbiology, Shandong Iniversity,Kinan 250100);Outline and Perspective of Research and Development of Bioactive Peptides from Marine Protein Enzymatic Hydrolysate[J];Amino Acids & Biotic Resources;2000-04
8 ZHOU Hua-wei~1, LIN Wei-tie~1, CHEN Tao~2(1.School of Bioscience and Bioengineering, South China University of Technology , Guangzhou510641, China ; 2. South China Sea Insititute of Oceanology , Chinese Academy of Science , Guangzhou 510301, China);The Heterotrophy and Applications of Chlorella[J];Amino Acids & Biotic Resources;2005-04
9 MIAO Jin lai 1, 2 , SHI Hong qi 1, 2 , LI Guang you 2, WANG Bo 2 & HAN Li 2 (1 Marine Life College, Ocean University of Qingdao, Qingdao 266003, China) (2 Key Laboratory of Marine Bio active Substances, State Ocean Admission, Qingdao 26;SOME RESEARCH ON THE WAYS OF RED TIDE PREDICTION AND PREVENTION[J];Journal of Safety and Environment;2002-03
10 CHENG Yu-hong,LI Zhi-rong,FU Qi (Department of Chemistry;Anyang Normal University;Anyang 455002,China);Study on the Eradicating Effect of Oxygen Free Radicals by Ginkgo-Biloba Extract[J];Journal of Anyang Normal University;2007-02
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 JIANG Hong-xia et al (College of Life Sciences,Henan Normal University,Xinxiang,Henan 453007);Studies on Antimicrobial Activities against Three Plant Pathogen by Using Eleven Microalgae Extracts[J];Journal of Anhui Agricultural Sciences;2008-10
2 SUN Cheng-bo~1, WANG Xiao-hua~1, BI Hui-qin~2(1. Profession Technological School,Tianjin Agricultural College ,Tianjin 300380,China; 2. The Bohai Sea Fishery Resources Reproduce Station,Tianjin Fishery Institute,Tianjin 300221,China);Commercial Culture of Marine One-celled Algae[J];Fisheries Science;2005-08
3 WANG xiao-qin,ZHANG xue-wu(College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,China);Optimization of Protein Extraction from Chlorella using Response Surface Methodology[J];Modern Food Science and Technology;2012-03
4 CHEN Xiao-qing1,ZHENG Yi2,LIN Xiong-ping3(1.Department of Biology Science and Technology,Zhangzhou Normal College,Zhangzhou 363000,Fujian,China;2.Fujian Higher Educational Key Laboratory of Development and Neural Biology,College of Life Sciences/Fujian Normal Univerity,Fuzhou 350108,China;3.Department of Biology Technology,Ningde College,Ningde 352100,Fujian,China);Purification and Some Characteristics of Antimicrobial Protein from Porphyridium cruentum[J];Hubei Agricultural Sciences;2011-08
5 JIANG Hong-xia1,ZHENG Yi2,LEI Hong-juan1,XUAN Wen-juan1(1.College of Life Sciences,Henan Normal University,Xinxiang 453007,China;2.College of Life Sciences,Fujian Normal University,Fuzhou 350108,China);Studies on Antimicrobial Activities against Plant Pathogen Using Four Microalgae Extracts[J];Journal of Henan Agricultural Sciences;2008-10
6 LIU Si-guang 1,LI Wen-quan 2,HUANG Zhi-wei 1,CHEN Lan 1,WANG Jian 1,ZHANG Fan 1 (1.Fujian Institute of Oceanology,Xiamen 361013,China;2.Department of Oceanography, Institute of Subtropical Oceanography,Xiamen University,Xiamen 361005,China);Preliminary study on in vitro antibacterial activity of polysaccharides from Chlorella autotrophica[J];Marine Science Bulletin;2012-01
7 YE Lin-Chao,YE Jun-An,XU Guo-Zhong,LIU Jian-Xin(College of Animal Science,Zhejiang University,Hangzhou,310029);ANIMAL PROTEIN ENZYMATIC HYDROLYSIS,N,AND P SOURCES:COMBINATION EFFECT ON GROWTH AND BIOCHEMICAL PARAMETERS OF CHLORELLA VULGARIS[J];Oceanologia Et Limnologia Sinica;2009-02
8 XUE Wei, LIU Yu-mei, SONG Bao-an, HU De-yu, YANG Song, LU Ping (Research and Development Center of Fine Chemicals, Key Laboratory of Green Pesticide and Agricultural Bioengineering of Ministry of Education, Guizhou University, Guiyang 550025, China);Antifungal Activity and Isolation Methods of Heterocyclic Natural Products[J];Agrochemicals;2007-11
9 OUYANG Ke-xian;LUO Xiao-yan;LIU Jian-ping;CAI Li-chuang;XIAO Zheng-qiang;Institute of Biological Resources,Jiangxi Academy of Sciences;Jiangxi science and Technology Institute;;Nutritional Value and Applications in Feed Industry of Chlorella[J];Jiangxi Science;2013-05
10 ZHANG Xue-Cheng1,SHI Yan-Xia1,MENG Zhen2(1.College of Marine Life Science,Ocean University of China,Qingdao 266003,China;2.Key Laboratory for Sustainable Utilization of Marine Fisheries Resource,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Science,Qingdao 266071,China);The UV-Irradiation of Chlorella. vulgaris Beijerinck and Screening of Productive Strain[J];Periodical of Ocean University of China;2007-05
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 She Min,He Gengxing,Chen Hui,et al (Ningbo Institute of Medical Sciences,Ningbo Zhejiang,315000);AN EXPERIMENTAL STUDY OF 5 KINDS OF HALIBIOS ON ANTIAGEING ACTION[J];Chinese Journal of Marine Drugs;1995-02
2 ;Nutritive Value,Production,Application and Development of Microalgae[J];CEREAL & FEED INDUSTRY;1997-05
3 Chen Laicheng (Institute of scientific and Technical information of China);A SURVEY OF DEVELOPMENT OF MARINE BIO-TECH ABROAD[J];PROGRESS IN BIOTECHNOLOGY;1994-06
4 Tang Yingzhong\ Huang Liqun (Wuhan Urban Construction Institute)\ (Hubei research Institute of Chemistry);A Review of Hydrocarbon Production From Algat[J];PROGRESS IN BIOTECHNOLOGY;1997-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved