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《东北农学院学报》 1993-04
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MORPHOLOGICAL AND HISTOCHEMICAL STUDIES ON THE CHANGES OF LIPID DROPLETS IN DEVELOPMENT OF OOCYTES OF THE MINK

Liu Yutang (Department of Wildlife Science of Northeast Forestry University, Harbin 150040) Qin Pengchun (Department of Biotechnology of Northeast Agricultural College, Harbin 150030, China)  
Mink oocytes of different development stages were studied with an electron microscope and histochemical techniques. In mink oocytes lipid droplet formation is one of the marks that indicates the beginning of growth of oocytes. In early development stage of oocytcs, lipid droplets are scattered in the ooplasm between the cortical region and nucleus and arc stained dark in color. But in the late stage their size increases markcdly.The droplets gather together, and arc stained light in color. Tt was found by staining that lipid droplets consisted mainly of neutral fat and phospholipid before the appcarance of the follicular antrum, but after the appearance they consisted mainly of neutral fat, and phospholipid disappeared. The results of the experiment showed that the lipid droplets came probably from a kind of black osmiophilic granules (with diameter about 0.38μm) in the quiet oocytcs. Possessing a lot of lipid droplets is one of the characteristics of mink's mature oocytcs.
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