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《JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY》 1999-03
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PURIFYING mRNA BY ONE STEP

LI Jing peng ,HE Cheng qang, WU Dan,REN Gui ping, LING Hua,LU Yun zhe (NEAU Agricultural University,Harbin 150030,PRC)  
RNAs of Hela cells, spleen of rabbit and common bean seedling were purified through one step with guandine isothiocyanate. mRNAs were separated from total RNA by affinity chromatography on oligo (dT)-cellulose. Prepared mRNA can be used as template for construction of cDNA libraries, and for reverse transeript PCR. $$$$
【Fund】: 黑龙江省科委攻关项目
【CateGory Index】: Q522
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