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《Journal of Northeast Agricultural University》 2000-03
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Establishment of agrobacterium rhizogenesis mediated transformed system on transferring virus resistant genes into tomato

WANG Ao xue,LI Jing fu,XU Xiang ling LI Gui ying,WANG Fu,XU Xiang yang (Northeast Agricultural University,Harbin 150030,PRC)  
Four factors,genotype,the densities of bacterial suspension,explants and transformed methods,were set up in this experiment.Target genes,TMV CP gene and CMV CP gene,were transferred into tomatoes according to orthogonal table L 16 (4 4×2 3)by agrobacterium rhizogenesis 。The results of Km resistant calli's frequency demonstrate:Significant differences exist between genotypes and between different densities of bacterial suspension,and Significant differences don't exist between explants and between different methods.9 transgenic tomatoes were gained.Through PCR and ELISA detection,TMV CP gene and CMV CP gene were proved to be transferred into tomatoes and expressed in the part of transgenic tomatoes.$$$$
【CateGory Index】: Q943.2
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