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《Journal of Northeast Agricultural University》 2005-04
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Feasibility study of lactic acid bacteria as host system

ZHAO Zhen-ao,XU Xiu,DENG Kai-bo,TANG Li-jie,LI Yi-jing ( Life Science College, Northeast Agricultural University, Harbin Heilongjiang 150030, PRC )  
In the study, we verified physiological and biological characteristics , antibiotic resistance, transformation and extract plasmid and identification e tc. Test to Lactobacillus, and we identified the feasibility of Lactococcus last ic NZ9000 and Lactobacillus acidophilus ATCC4356 as host system. We can get a co nclusion that Lactococcus lastic NZ9000 and Lactobacillus acidophilus ATCC4356 c ould be as the host system and express the shuttle expression plasmid pLP352(chl oromycetin resistance) between E. coli. and lactic acid bacteria. Our study have laid the ground work for some useful combat disease gene expressed in the lacti c acid bacteria, and testified the feasibility of Lactobacillus as biological sa fe grade host system.
【Fund】: 国家自然科学基金项目(30371074);; 东北农业大学科技创新基金
【CateGory Index】: Q939.99;
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