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《Journal of Northeast Agricultural University》 2006-02
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Genetic diversity of Chinese melon cultivars(Cucumis melo L.) based on simple sequence repeat markers

SHENG Yunyan,LUAN Feishi,CHEN Kenong(Horticulture College,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC)  
The genetic diversity among 46 Chinese melon cultivars(Cucumis melo L.) from different area in China was assessed by variation of 50 simple sequence repeat primers.It resulted that there were total 46 polymorphic bands on 48 amplified bands using fifty primers of simple sequence repeat.Total 187 alleles,which 130 of them had polymorphism,revealed the average of 3.729 alleles per marker,and the molecular size was between 250-1 750 bp.46 Chinese melon cultivars were divided into nine groups using NTSYS program and methods of multidimensional scaling and cluster tree analysis.21 characterization melon accessions from 9 different groups were selected to analysis again,they were divided into 8 groups.There were the same results on 15 melon accessions,6 melon accessions had different conclusion after compared two methods of MDS and cluster tree analysis by NTSYS program.It showed difference between two analyses,and two methods had advantage respectively.Moreover,these date indicated that genetic diversity of Chinese melon cultivars could be enhanced by the introduction of genetic variation from different origins of Chinese accessions,at the same time it would be advantageous to acquire more accessions from this geographically varied region to ensure the retention of existing genetic diversity.SSR markers play an important role in identification and study of genetic diversity.
【Fund】: 黑龙江省农业科学院博士后资助项目
【CateGory Index】: S652
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