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《Journal of Northeast Agricultural University》 2006-03
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Application of in silico cloning technique in plant gene engineering

WANG Dongdong,ZHU Yanming,LI Yong,LI Jie,BAI Xi(College of Life Science,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC)  
In silico cloning was a new technique of gene-cloning which developed with genome project and EST project via bioinfomatics method.It has many advantages,such as low investment,high pertinence,and it is fast,easy to operation,It will be the main strategy of functional genes cloning in plant gene engineering.This paper reviewed the principle of in silico cloning,related resource of database and bioinformatics,application and prospect of in silico cloning in plant gene engineering.
【Fund】: 国家自然科学基金(30471059)
【CateGory Index】: Q943.2
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Chinese Journal Full-text Database 10 Hits
1 JIANG Sheng-juan,SUN Yu-jun,ZHANG Qiang,DUAN Li-jun(College of Life Science,Anhui Science and Technology University,Fengyang 233100,China);In Silico Cloning and Bioinformatics Analysis of Allergy Ara h 1 Gene from Peanut[J];Journal of Anhui Science and Technology University;2009-02
2 Yue Guidong Sui Zhenhua Li Xu Zhang Juren (School of Life Science,Shandong University,Jinan 250100);Molecular cloning of maize PIS2 and MFSP with bioinformatics databases[J];Chinese High Technology Letters;2009-06
3 KONG Ling-fang,ZHANG Ji-yu,LIU Zhi-peng,WANG Yan-rong(College of Pastoral Agriculture Science and Technology,Lanzhou University;State Key Laboratory of Grassland Agro-ecosystems;The Key Laboratory of Grassland Agro-Ecosystems of Ministry of Agriculture,Lanzhou 730020,China);Cloning of a S-adenosyl methionine synthetase gene from Cleistogenes songorica and its expression under drought stress[J];Acta Prataculturae Sinica;2013-01
4 LI Meng,ZHANG Ai-hua,BAI Xue-fei,WANG Cui-xia,DUAN Hui-jun(Agricultural University of Hebei,Key Laboratory of Crop Germplasm Resources of Hebei,Baoding 071001,China);Silico Cloning and Bioinformatics Analysis of Zeaxanthin Epoxidase Gene(ZE) in Cucunis melo[J];Acta Agriculturae Boreali-Sinica;2011-S1
5 LI Wen-shuang1,LIU Yuan1,CHANG Wen-suo1,LIU Meng-xing2,LI Xi-huan1(1.North China Key Laboratory of Crop Germplasm Resources,Education Ministry of China,Agriculture University of Hebei,Baoding 071001,China;2.Agriculture University of Hebei,Baoding 071001,China);In SilicoCloning and Bioinformatics Analysis of GmMDH1 Gene in Soybean (Glycine max.L)[J];Acta Agriculturae Boreali-Sinica;2012-03
6 SUN Yan-xiang LI Ping;In Silico Cloning and Analysis of Tonoplast H~+-ATPase A subunit cDNA in Medicago truncatula[J];Journal of Langfang Teachers College(Natural Science Edition);2009-01
7 FENG Chao1) LEI Ying2) LIU Yong-zhong1)(1)Key Laboratory of MOE for Horticultural Plant Biology,College of Horticulture and Forestry Science,Huazhong Agricultural University,Wuhan 430070,China;2)State Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China);Cloning of a Citrus L-galactono-1,4-lactone Dehydrogenase Gene[J];Journal of Huazhong Agricultural University;2009-06
8 GONG Yuan-yong1,GUO Shu-qiao1,SHU Hong-mei1,HE Lin-chi2,NI Wan-chao1(1.Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Cotton and Rapeseed,Ministry of Agriculture,P.R.China,Nanjing 210014,China;2.Institute of Agricultural Sciences of Jiangsu Changjiang River Bank District,Rugao 226541,China);In silico cloning and bioinformatics analysis of aquaporin gene GhNIP5.1 from upland cotton(Gossypium hirsutum)[J];Jiangsu Journal of Agricultural Sciences;2013-03
9 YI Le-fei 1,LIU Chu-wu 2,WANG Ping 1,ZHOU Xiang-hong 1 (1.Jiangsu Key Laboratory of Marine Biotechnology,Huaihai Institute of Technology,Lianyungang 222005,China;2.Fisheries College,Guangdong Ocean University,Zhanjiang 524088,China);cDNA cloning and characterization of a novel ubiquitin-conjugating enzyme gene from Porphyra yezoensis Ueda[J];Journal of Fisheries of China;2009-05
10 YANG Yong1,MA Chang-wei2,ZHAO Chun-jiang3,WU Chang-xin3(1.College of Food Science,Sichuan Agricultural University,Yaan 625014,Sichuan,China;2.College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China;3.College of Animal Science and Technology,China Agricultural University,Beijing 100094,China);Cloning and Verification of ATGL Gene in Mini-pig and Comparison of the Coding Sequence in Different Porcine Breeds[J];Journal of Sichuan Agricultural University;2011-02
Chinese Journal Full-text Database 6 Hits
1 Wan Yuehua and He Limin (Library of Zhejiang University of Technology,Hangzhou 310032);Bioinformatics Database Resources on Internet[J];Journal of The China Society For Scientific and Technical Information;2002-04
2 ZHANG Cheng-Gang ** ,HE Fu-Chu (Department of Genomics and Proteomics,Beijing Institute of Radiation Medicine,Beijing,100850,China;;Application of Bioinformatics in Full-length cDNA Sequence Analysis and Function Prediction of Novel Genes*[J];Progress In Biochemistry and Biophysics;2003-01
3 Wan Haiwei 1 Du Lixin 2 ( 1 College of Animal Sciences Shandong Agricultural University,Taian 271018 ; 2 Institute of Animal Husbandry Chinese Academy of Agricultural Science, Beijing 100094);Application of EST in the Study of Genomics[J];Biotechnology Information;2004-01
4 ;NCBI基因序列数据库使用和检索方法[J];Modern Information;2003-12
5 HUANG Ji 1, ZHANG Hong sheng 1,* , CAO Ya jun 1, QIAN Xiao yin 2, YANG Jin shui 2 ( 1National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2Institute of Genetics, Fudan;Strategy of in Silico Cloning of Functional Genes in Rice (Oryza sativa)[J];Chinese Journal of Rice Science;2002-04
6 HUANG Ji WANG Jian-Fei ZHANG Hong-Sheng②(National Key Laboratory of Crop Genetics & Germplasm Enhancement,Nanjing Agricultural University, Nanjing 210095);Advances on Plant Pentose Phosphate Pathwayand Its Key Enzymes[J];Chinese Bulletin of Botany;2004-02
Chinese Journal Full-text Database 10 Hits
1 SHI Dong-yan1,CHENG Zai-quan2(1.Biology Department of Heze College,ShandongHeze 274015,China;2.Biotechnology & Genetic Germplasm Institute,Yunnan Academy of Agricultural Sciences,Kunming 650203,China);The isolation and annotation of some cDNA fragments of yunnan Oryza meyeriana[J];Journal of Anhui University(Natural Sciences);2008-03
2 Li Xiao-xiao et al(College of Life Science,Capital Normal University,Beijing 100037);In Silico Cloning and Evolution Analysis of Geranylgeranyl Hydrogenase Gene from Lotus corniculatus var.Japonicus[J];Journal of Anhui Agricultural Sciences;2006-23
3 MENG Xian-ping et al(College of Life Science,Capital Normal University,Beijing 100037);In silico Cloning of Gamma-tocopherol Methyltransferase Gene from Lotus corniculatus var.japonicus[J];Journal of Anhui Agricultural Sciences;2006-24
4 ZHANG Shu-lin et al (South Western Forestry College,Kunming,Yunnan 650224);Applications of Biological Information in Agriculture[J];Journal of Anhui Agricultural Sciences;2007-22
5 CHEN Zhuo et al(Department of Biology Engineering and Technology,Qingdao Science and Technology University,Qingdao,Shandong 266042);Analysis on Expressed Sequence Tags(EST) and Research Progress of Algae EST Library[J];Journal of Anhui Agricultural Sciences;2009-05
6 YANG Hui et al(College of Life Science,China West Normal University,Nanchong,Sichuan 637002);Expression Differences in TP Mutation of Wheat under Light Stress[J];Journal of Anhui Agricultural Sciences;2010-07
7 REN Xiu-yan et al (College of Life Science,Langfang Teacher's College,Langfang,Hebei 065000);Analysis on the Protein Structure and Function of HpaG_(Xoo)[J];Journal of Anhui Agricultural Sciences;2010-09
8 WANG Xiao-bo et al(College of Agronomy,Anhui Agricultural University,Hefei,Anhui 230036);Prediction of Biological Function of Plasma Membrane Aquaporins in Soybean[J];Journal of Anhui Agricultural Sciences;2010-34
9 ZHANG Qi-wei1, LI Lei1, ZHAO Dong-chi1, ZHANG Chu-yu 1*, YOU Shang-you2, LI Le2, HUANG Jing-ning2 (1. State Key Laboratory of Virology,Wuhan University,Wuhan 430072,China 2. Wuhan Children's Hospital, Wuhan 430020,China);In Silico Cloning and Analysis of zzz171con Gene in SPC-A1 Cells Responsive to Human RSV Infection[J];Amino Acids & Biotic Resources;2005-02
10 WEI Xiao-chun,ZHENG Qun(Horticultural Department,Shihezi University,Shihezi,Xinjiang 832000,China);The Application of the Bioinformatics in Finding New Genes[J];Northern Horticulture;2009-05
China Proceedings of conference Full-text Database 1 Hits
1 LIU Ju-Hua JIN Zhi-Qiang XU Bi-Yu (National Key Laboratory of Biotechnology and Science for Tropical Crops);Research Progress of Plant Functional Genomics and Future Application on Tropical Crops[A];[C];2004
Chinese Journal Full-text Database 10 Hits
1 Sun Linlin Jiang Jizhi LiLi (College of Life Sciences,Hebei University,Baoding 071002,China);Bioinformatics and its applications in study of crop resistant gene[J];Anhui Agricultural Science Bulletin;2006-10
2 Nan zhirun fan yuexian(Department of art and science,shanxi agriculture of university,taigu 030801);Advance of Researches on Catalase in Plants[J];Anhui Agricultural Science Bulletin;2008-05
3 Li yeyu et al.(Department of life science,Nanjing Xiaozhuang college,Nanjing 211171,China);Bioinformatic Analysis of catalase Gene from Hypericum perforatum[J];Anhui Agricultural Science Bulletin;2009-13
4 Gao Derong et al (Lixiahe Regional Agricultural Research Institute, Yangzhou, Jiangsu 225002);Breeding Strategies of Wheat with the Resistance to Powdery Mildew[J];Journal of Anhui Agricultural Sciences;2001-05
5 Gao Junshan et al (Anhui Agricultural University,Hefei 230036);Summary of the Techniques and Methods of Plant Gene Transformafion[J];Journal of Anhui Agricultural Sciences;2003-05
6 LIN Li et al (Biotech College of Shenyang Agricultural University,Shenyang,Liaoning 110161);Research Advance on Plant Elicitors[J];Journal of Anhui Agricultural Sciences;2006-22
7 NIU Xu-guang et al(College of Land and Environment,Shenyang Agricultural University,Shenyang,Liaoning 110161);In Silico Cloning and Expression Analysis of an Inositol 1,3,4-Trisphosphate 5/6-Kinase Gene in Oryza sativa[J];Journal of Anhui Agricultural Sciences;2006-24
8 DU Qi-yan et al(College of Life Science,Henan Normal University,Xinxiang,Henan 453007);GH cDNA Clone and Pronucleus Expression of Misgurnus Anguillicaudatus and Paramisgurnus Dabryanus[J];Journal of Anhui Agricultural Sciences;2007-02
9 QUAN Wei et al(Hybrid Wheat Engineering and Technique Research Center,Beijing Municipal Academy of Agriculture and Forestry Sciences,Beijing 100097);Research Advance in the Fertility Alteration of the Photo-thermo-sensitive Male Sterile Wheat[J];Journal of Anhui Agricultural Sciences;2007-21
10 ZHANG Shu-lin et al (South Western Forestry College,Kunming,Yunnan 650224);Applications of Biological Information in Agriculture[J];Journal of Anhui Agricultural Sciences;2007-22
China Proceedings of conference Full-text Database 1 Hits
1 ZHANG Zheng-feng, CHEN Hong,LI Qiu-ling , LEI Chu-zhao, ZHANG Chun-lei, XUE Kai,Wang Yi-min, WANG Xin-zhuang (College of Animal Science and Technolog, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China;Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, Jiangsu 221116, China;The Research Center of Cattle Engineering Technology in Henan, Zhengzhou, Henan 450003, China);Study on the Polymorphisms of DGAT2 Gene and Its Associations with Several Growth Traits in Nanyang Cattle[A];[C];2006
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 YI Le-fei et al(School of Marine Science & Technology,Huaihai Institute of Technology,Lianyungang,Jiangsu 222005);Cloning and Analysis of BADH Gene from Helianthus annuus[J];Journal of Anhui Agricultural Sciences;2008-32
2 YI Le-fei1,2,LI Xin-shu2,YAN Bin-lun1(1.Jiangsu Key Laboratory of Marine Biotechnology,Huaihai Institute of Technology,Lianyungang 222005,China; 2.School of Marine Science & Technology,Huaihai Institute of Technology,Lianyungang 222005,China);Comparison of Methods for Simultaneous Extraction of DNA and RNA from Seaweeds[J];Fisheries Science;2011-11
3 ZHOU Xiang-hong,PENG Yong-xing,YI Le-fei(School of Marine Science & Technology,Huaihai Institute of Technology,Lianyungang,Jiangsu 222005);In silicocloning and sequence analysis of HSC70 from Poecilia reticulata Peters[J];Freshwater Fisheries;2011-06
4 SHANG Xiao-hong 1,2,TIAN Zhen-dong 1 *,LIU Jun1,XIE Cong-hua1(1 College of Horticulture& Forestry Sciences,Huazhong Agricultural University/National Center for Vegetable Improvement(Center China) /Potato Engineering and Technology Research Center of Hubei Province,Wuhan 430070,China;2 Vegetable Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China);Mapping of gene related to late blight resistance in potato and QTL analysis[J];Journal of Southern Agriculture;2011-07
5 LIU Ya-ling1,YANG Hong-yan1,ZHANG Lin1,WANG Zhao-ming1*, YUN Jin-feng2,CUI Hai-peng1,YUAN Feng1(1.Inner Mongolia Hotision Monsod Drought-Resistance Greening Co.,Ltd,Huhhot,Inner Mongolia 010030 China; 2.College of Ecology and Environmental Science,Inner Mongolia Agricultural Universitv,Huhhot,Inner Mongolia 010019,China);Cloning and Sequence Analysis of Ubiquitin-conjugating Enzyme Gene from Wild Carex duriuscula[J];Acta Agrestia Sinica;2012-04
6 DENG Ke-jun1,DU Mei-ze1,LU Ji2,and REN Zheng-long1(1.School of Life Sciences and Technology,University of Electronic Science and Technology of China Chendu 610054;2.Sichuan Provincial Department of Agriculture Horticultural Crops Extension Center Chendu 610041);In Silico Cloning and Characterization of the SAMDC Gene in Salvia Miltiorrhiza Bge[J];Journal of University of Electronic Science and Technology of China;2012-05
7 KONG Ling-fang,ZHANG Ji-yu,LIU Zhi-peng,WANG Yan-rong(College of Pastoral Agriculture Science and Technology,Lanzhou University;State Key Laboratory of Grassland Agro-ecosystems;The Key Laboratory of Grassland Agro-Ecosystems of Ministry of Agriculture,Lanzhou 730020,China);Cloning of a S-adenosyl methionine synthetase gene from Cleistogenes songorica and its expression under drought stress[J];Acta Prataculturae Sinica;2013-01
8 LI Ling-ling,Lü Zao-sheng,LI Tao,SHEN Hui(College of Chemical Engineering and Technology,Wuhan University of Science and Technology,Wuhan 430081,China);Expression and Sequence Analysis of Mutant Ketoreductase Domain of Polyketide Synthase[J];Chemistry & Bioengineering;2011-08
9 LIN Shi-feng1,REN Xue-liang1,WANG Yi1,WANG Dong-mao2,ZHANG Tuo2,WANG Ren-gang1 (1.Guizhou Tobacco Research Institute,Guiyang 550081,China;2.State Key Laboratory of Proteomics,Beijing Proteomics Research Center,Beijing 102206,China);Cloning and Sequence Analysis of Three Betaine Aldehyde Dehydrogenase Genes in Tobacco (Nicotiana tabacum)[J];Acta Agriculturae Boreali-Sinica;2012-03
10 ZHOU Xiang-hong1,2,LI Xin-shu2,YAN Bin-lun1,YI Le-fei1,2,WANG Ping2(1.Jiangsu Key Laboratory of Marine Biotechnology,Huaihai Institute of Technology,Lianyungang 222005,China;2.School of Marine Science & Technology,Huaihai Institute of Technology,Lianyungang 222005,China);Response of Cysteine Synthase Gene Expression to Lead Stress in Porphyra yezoensis Ueda[J];Journal of Henan Agricultural Sciences;2011-10
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Lin Shanzhi; Li Xueping; Zhang Zhiyi.Institute of Populus tomentosa, Beijing For. Univ.,100083,P.R.China.;Changes of content of CaM, and activities of G6PDHase and ATPase during low-temperature-induced freezing tolerance of Populus tomentosa seedlings.[J];Journal of Beijing Forestry University;2001-05
2 Han Jianguo; Pu Xinchun; Mao Peisheng (Grassland Institute, China Agricultural University, Beijing 100094);Changes of Some Enzyme Activities in Tall Fescue Seed During Germination Under Accelerated Ageing[J];ACTA AGRESTIA SINICA;1998-02
3 WANG Dong 1,2 , YANG Jin shui 1 (1. Institue of Genetics, School of Life Sciences, Fudan University, Shanghai, 200433, China; 2. Department of Crop Genetics and Breeding, Nanjing Agriculture University, Nanjing 210095, China);Cloning and Characterization of cDNA Encoding Cotton STZ-like Protein[J];Journal of Fudan University;2002-01
4 PENG Ke qin 1, XIA Shi tou 1, LI Yang sheng 2 (1.College of Sciences,HNAU,Changsha 410128, PRC; 2.Changsha Institute of Agricultural Modernization, Academia Sinica, Changsha 410125,PRC);Effects of Complete Submergence on Some Physiological and Yield Characteristics of Early and Middle-Season Rice[J];JOURNAL OF HUNAN AGRICULTURAL UNIVERSITY;2001-03
5 GUO Xin hong 1,JIANG Xiao cheng 1,PAN Xiao ling 2,CHEN Liang bi 1,ZHOU Guang qia 1 (1.Life Science College,Hunan Normal University,Changsha,410081,Hunan,China; 2.Insititute for Researching Arid Ecological Environment of Xinjiang University,xin;SSH Method and Its Application in Gene Cloning[J];Acta Laser Biology Sinica;2001-03
7 PENG Yong_de, SONG Huai_dong, GAO Guo_feng, et al (Shanghai Institute of Endocrinology, Ruijin Hospital, SSMU, Shanghai?200025, China);The Splicing Characteristics and Biological Significance of New Isoforms in Novel Full-Length cDNA Cloning[J];Acta Universitatis Medicinalis Secondae Shanghai;2001-04
8 WANG Hua Chun,CHEN Qing Xuan(Institute of Developmental Biology ,The Chinese Academy of Sciences,Beijing 100080, China).;Get the Best Use of dbEST.[J];PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS;2000-04
9 WANG Ji Cun,YAO Li Bo ** ,ZHAO Zhong Liang (Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xian 710032,China);The Progress of the Methods for Screening Differentially Expressed Genes and Proteins[J];Progress In Biochemistry and Biophysics;2001-01
10 ZHANG Cheng Gang, OUYANG Shu Guang, ZHANG Shao Wen, QU Xiang Hu, YU Yong Tao, ZHOU Gang Qiao, WU Song Feng, HE Fu Chu  (Department of Genomics and Proteomics, Beijing Institute of Radiation Medicine, Beijing 100850, China).;Construction and Application of a Large-scale DNA Sequence Analysis System Based on PC/Linux[J];Progress In Biochemistry and Biophysics;2001-02
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