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《Journal of Northeast Agricultural University》 2007-01
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Optimal condition for simple sequence repeat in common bean (Phaseolus vulgaris L)

FENG Guojun1,XU Qijiang2,LI Yuhua2,LIU Dajun1 (1. Harbin Academy of Agricultural Sciences, Harbin 150070, China; 2. Colllege of Life Sciences, Northeast Forestry University, Harbin 150040, China)  
Simple sequence repeat (SSR) is an important technique of molecular markers for main agronom-ical traits and marker-assistant breeding in crop breeding. Six cultivars of Zihua, Bayuelu, Jiangjun, 73-8, 96-9, and 9z622 were used to extract high-quality genomic DNA by using the improved SDS method. In order to establish the optimized SSR detection system, the concentrations of DNA, Mg~(2+), Taq DNA polymerase, dNTPs, and the annealing temperature were optimized for SSR-PCR reaction. The optimal conditions were 0.8 ng·μL~(-1) DNA, 1.5 mmol·L~(-1) Mg~(2+), 0.05 μmol·(μL·min)~(-1) Taq DNA polymerase, and 0.2 mmol·L~(-1) dNTPs in a 25 μL SSR-PCR reaction mixture.
【CateGory Index】: S643.1
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