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《Journal of Northeast Agricultural University》 2007-01
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Optimal condition for simple sequence repeat in common bean (Phaseolus vulgaris L)

FENG Guojun1,XU Qijiang2,LI Yuhua2,LIU Dajun1 (1. Harbin Academy of Agricultural Sciences, Harbin 150070, China; 2. Colllege of Life Sciences, Northeast Forestry University, Harbin 150040, China)  
Simple sequence repeat (SSR) is an important technique of molecular markers for main agronom-ical traits and marker-assistant breeding in crop breeding. Six cultivars of Zihua, Bayuelu, Jiangjun, 73-8, 96-9, and 9z622 were used to extract high-quality genomic DNA by using the improved SDS method. In order to establish the optimized SSR detection system, the concentrations of DNA, Mg~(2+), Taq DNA polymerase, dNTPs, and the annealing temperature were optimized for SSR-PCR reaction. The optimal conditions were 0.8 ng·μL~(-1) DNA, 1.5 mmol·L~(-1) Mg~(2+), 0.05 μmol·(μL·min)~(-1) Taq DNA polymerase, and 0.2 mmol·L~(-1) dNTPs in a 25 μL SSR-PCR reaction mixture.
【CateGory Index】: S643.1
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【References】
Chinese Journal Full-text Database 3 Hits
1 GU Yu,HAN Qi-hou,WANG Wu-tai,LI Su-wen,SUN De-lingand WU Feng (Tianjin Kernel Vegetable Research Institute,Tianjin 300382,China);Application of Common Bean Anthracnose Resistance Gene SCAR Markers in Snap Bean Disease Resistance Identification[J];园艺学报;2011-05
2 SHENG Xin-ying1,ZHAN Shao-hua2,FAN Hong-hong1,CAI Yong-ping1,LIN Yi1(1.School of Life Science,Anhui Agricultural University,Hefei 230036;2.Chemistry and Biology Department,West Anhui University,Liu'an 237012,Anhui,China);Purity Identification of "Meiqiao" Soybean(Glycine max) by SSR Markers[J];大豆科学;2010-02
3 BAI Jin-rong,PAN Hui-tang,ZHANG Qi-xiang(College of Landscape Architecture,Beijing Forestry University,National Engineering Research Center for Floriculture,Beijing 100083,China);Optimization and Establishment of SSR Analysis System in Roses(Rosa spp.)[J];华北农学报;2009-03
【Citations】
Chinese Journal Full-text Database 4 Hits
1 YAO Qing-rong1, 2, ZHAO Chang-zeng1,WANG Wen-quan2 (1. Department of Horticulture, Gansu Agricultural University,Gansu, Lanzhou 730070;2. National Key Biotechnology Laboratory for Tropical Crops, CATAS, Haikou 571101, China);Extraction of genomic DNA and optimization of SSR amplified condition in litchi[J];甘肃农业大学学报;2004-02
2 DUAN Xi-Hua 1LUAN Fei-Shi 2 SUN Jing-Bo 3 (1.Northeast Foreast University,Harbin 150040) (2.Northeast Agricultural University,Harbin 150030) (3.Forestry Bureau of Heilongjiang,Harbin 150030);The morphology and allozyme of germplasm resources on Phaseolus vulgaris[J];植物研究;2004-01
3 Zheng Jingsheng 1,2 Lü Bei 1 1 Hainan Provincial Key Laboratory of Crop Molecular Breeding,Sanya,572025 2 The Institute of Rice&wheat,Fujian Academy of Agricaltural Sciences,Fuzhou,350019;PCR Technique and its Practical Methods[J];分子植物育种;2003-03
4 LUAN Fei-shi 1 ZU Yuan-gang 2 (1. Northeast Agricultural University, Harbin 150030) ( 2. Northeast Forestry University, Harbin 150040);RAPD DIVERSITY OF GERMPLASM RESOURCES ON PHASEOLUS VULGARIS I[J];植物研究;2002-04
【Co-citations】
Chinese Journal Full-text Database 9 Hits
1 WANG Yong-juan;ZUO Wei-yong;ZHU Shan-yuan;WANG An-ping;WU Shuang;HONG Wei-ming;LU Hui;Jiangsu Agri-animal Husbandry Vocational College/Jiangsu Provincial Key Laboratory of Veterinary Bio-pharmaceutical High Tech Research;;Establishment of a RT-PCR method for duck reovirus detection[J];江苏农业学报;2016-05
2 PANG Fengjiao;ZHANG Baimeng;HE Kongwang;YU Zhengyu;XU Xiangwei;GUO Rongli;FAN Baochao;WEN Libin;LI Bin;JIANG Ping;College of Veterinary Medicine,Nanjing Agricultural University;The Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences;;Development and application of a RT-PCR method based on M-gene for detection of porcine Deltacoronavirus[J];畜牧与兽医;2016-09
3 LI Yi;YIN Quan;LIU Yong;Sichuan University for Nationalities;Analysis and Testing Center,Sichuan Academy of Agricultural Sciences;Plant Protection Research Institute,Sichuan Academy of Agricultural Sciences;;Quadruple PCR detection of herbicide-resistant transgenic rapeseed T45[J];南方农业学报;2016-06
4 LI Yi;YIN Quan;Sichuan University for Nationalities;Analysis and Testing Center,Sichuan Academy of Agricultural Sciences;;Detection of Transgenic Maize Bt11 with Insect Resistance and Herbicide Tolerance Based on MPCR[J];贵州农业科学;2016-06
5 Yi LI;Quan YIN;Sichuan Minzu College;Analysis and Testing Center,Sichuan Academy of Agricultural Sciences;;Detection of Herbicide-tolerant Canola by Multiplex PCR[J];Agricultural Science & Technology;2016-04
6 YIN Quan;LI Yi;SONG Jun;WANG Dong;ZHANG Fu-li;LIU Wen-juan;CHANG Li-juan;LEI Shao-rong;LIU Yong;Analysis and Testing Center, Sichuan Academy of Agricultural Sciences;Sichuan University for Nationalities;Institute of Plant Protection , Sichuan Academy of Agricultural Sciences;;Multiplex PCR for Detection of Four Transgenic Corns[J];湖北农业科学;2016-06
7 ZHANG Wenlin;ZUO Lihui;REN Yachao;LIU Hongmei;YANG Minsheng;WANG Jinmao;College of Forestry,Agricultural University of Hebei;;Effect of different treatments on Ulmus plant DNA extraction from leaves[J];河北林果研究;2015-04
8 Qingcai ZHAN;Guoqi ZHU;Jingping CHEN;Yijie ZHAN;Dan XUE;Agricultural Biotechnology Research Center of Hunan Province;Hunan Rice Research Institute;College of Applied Arts and Science of Beijing Union University;;Analysis on the Different Results of Seed Purity Identification for the Same Hybrid Rice Variety Using Different SSR Primers[J];Agricultural Science & Technology;2015-12
9 WANG Yun-bin;JIANG Liang-rong;HUANG Rong-yu;HUANG Yu-min;ZHENG Jing-sheng;School of Life Sciences, Xiamen University;;A High-efficiency and Low Cost Method of DNA Silver Staining in Non-denaturant Polyacrylamide Gel Electrophoresis——An Example Based on Rice[J];福建稻麦科技;2015-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHAN Shao-hua1,SHENG Xin-ying2 ,FAN Hong-hong2 ,CAI Yong-ping2 ,LIN Yi2(1Chemistry and Biology Department,West Anhui University,Lu'an 237012,Anhui; 2Life Science School,Anhui Agricultural University,Hefei 230036,Anhui,China);Relationship between Length of Soybean ESTs Sequence and Characters of EST-SSR[J];大豆科学;2009-02
2 WANG Kun,WANG Xiao-Ming,ZHU Zhen-Dong,ZHAO Xiao-Yan,ZHANG Xiao-Yan,and WANG Shu-Min Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement,Beijing 100081,China;Mapping of a Novel Anthracnose Resistance Gene Using SSR Markers in Common Bean(Phaseolus vulgaris L.)[J];作物学报;2009-03
3 LI Mei(Dalian Institute of Agricultural Sciences,Dalian 116036,China);Primary Analysis of Anti-disease Gene in Anthracnose Resistant Breeding of Bean[J];天津农业科学;2008-06
4 TIAN Lei,GUAN Rong-xia,LIU Zhang-xiong,CHANG Ru-zhen,QIU Li-juan(National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laborartory of Germplasm & Biotechnology,Ministry of Agriculture/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081);Verity Identification of Soybean Hybrids Using SSR Markers[J];植物遗传资源学报;2008-04
5 QIN Jun2,3,LI Ying-hui1,LIU Zhang-xiong1,GUAN Rong-xia1,ZHANG Meng-chen3,CHANG Ru-zhen1,LI Guang-min2.3,MA Zhi-ying2,QIU Li-juan1(1The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Germplasm and Utilization,Ministry of Agricuiture/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081;2Deprtment of Agronomy,Agricultural University of Hebei,Baoding 071000,Hebei;3 Hebei Academy of Agricultural and Forestry Science,Shijiazhuang 050031,Hebei);Genetic Relationship Among Parents of Elite Soybean (Glycine max) Cultivars Suinong14 Pedigree Revealed by SSR Markers[J];中国农业科学;2008-12
6 ZHAN Shao-hua et al(Department of Chemistry and Life Science,Wanxi University,Lu'an,Anhui 237012);Study on Extraction and Purification Methods of Soybean Genomic DNA[J];安徽农业科学;2008-23
7 WANG Kun,WANG Xiao-ming,ZHU Zhen-dong,ZHANG Xiao-yan,WANG Shu-min (National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081);Identification of Colletotrichum lindemuthianum Races and Bean Germplasm Evaluation for Anthracnose Resistance[J];植物遗传资源学报;2008-02
8 LI Ju-fen1, XU Ling1, MA Guo-bin2** (1. School of Life Sciences, East China Normal University, Shanghai 200062, China; 2.Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Protected Horticultural Technology,Shanghai 201106, China);Identification of Melon Hybrid Purity by SSR Markers[J];农业生物技术学报;2008-03
9 MA Ying-ying,WANG Chang-you,JI Wan-quan,WANG Qiu-ying,LIU Xue-li(College of Agronomy,Northwest Agricultural and Forestry University,Yangling Branch of China Wheat Improvement Center,Yangling 712100,China);SSR Analysis of Powdery Mildew Resistance Gene in New Wheat Germplasm N95175[J];华北农学报;2008-01
10 LI Yi1, 2, TIE Shuang-gui1, ZHU Wei-hong1, et al. (1. College of Life Sciences, Henan Normal University, Xinxiang 453000; 2. The Cereal Crops Institute, Henan Academy of Agricutural Sciences, Zhengzhou 450002, China);Selection of SSR Markers for Zhengdan 958 Maize Hybrid Seed Purity Identification[J];玉米科学;2008-01
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 XIE Dong-wei;LU Ying;YANG Xue;ZHAO De-bao;SUN Jian;The Institute of Industrial Crops,Heilongjiang Academy of Agricultural Sciences;College of Agronomy,Northeast Agricultural University;;Research Progress on Related Genes of Resistance to Anthracnose in Plant[J];华北农学报;2015-S1
2 Lin Chunjing;Zhang Chunbao;Dong Yingshan;Agricultural Biotechnology Institute, Jilin Academy of Agricultural Sciences;Soybean Institute, Jilin Academy of Agricultural Sciences;;Application of DNA Molecular Marker Techniques in Hybrid Seeds Purity Identification of Crop[J];分子植物育种;2015-03
3 Ma Wenjian;Zheng Lei;Zhang Jing;Liu Yang;Zhu Tianhui;College of Forestry,Sichuan Agricultural University;;Development of nested-PCR for detection of Cryphonectria parasitica based on the marker of sequence characterized amplified region[J];浙江大学学报(农业与生命科学版);2015-01
4 Hao Junjie;Zhang Xiaoyan;Wan Shuwei;Li Hongwei;Zhao Aihong;Sun Jilu;Wang Zhenqing;Qingdao Academy of Agriculture Science;Qingdao Agricultural Products Quality Inspection Center;;Molecular Marker-Assisted Selection for Pyramiding Angular Leaf Spot Resistance Genes in Common Bean(Phaseolus vulgaris L.)[J];作物杂志;2014-06
5 DING Xiao-liu;LIU Jia;ZHAO Hong-xia;WANG Jing;LUO Le;PAN Hui-tang;ZHANG Qi-xiang;Beijing KeyLaboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding,National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University;;Hybrid identification and morphological evaluation of modern roses( Rosa hybrida )×Rosa rugosa[J];北京林业大学学报;2014-05
6 JIA Xin-ping;YE Xiao-qing;LIANG Li-jian;DENG Yan-ming;SHE Jian-ming;SUN Xiao-bo;Institute of Agro-biotechnology,Jiangsu Academy of Agricultural Sciences,Provincial Key Laboratory of Agrobiology;;Extraction of genomic DNA and establishment of SSR reaction system for Davallia bullata[J];江苏农业学报;2013-06
7 DUAN Can-xing;ZHU Zhen-dong;SUN Su-li;WANG Xiao-ming;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement;;Advances in Study on Food Legumes Resistance Breeding in China[J];中国农业科学;2013-22
8 Wu Xingbo;Yue Huan;Hao Junjie;Zhang Xiaoyan;Li Hongwei;Wang Zhenqing;Lü Xianghua;College of Horticulture and Landscape Architecture,Southwest University;Qingdao Academy of Agriculture Science;;Identification of Rust Resistant Genes Based on SCAR Markers in Common Bean(Phaseolus vulgaris L.)[J];生物技术通报;2013-10
9 Yang Jing Zhang Guangchen * Jilin Agricultural University, Changchun, 130118;Optimization of ISSR-PCR Reaction System and Its Verification in Common Bean[J];分子植物育种;2013-04
10 Yan Zesheng,Jiang Leiyu,Zhang Zhongdian(College of Horticulture,Sichuan Agricultural University,Ya'an 625014,Sichuan,China);Comprehensive Evaluation of 32 Kidney Bean Germplasm Resources[J];农学学报;2013-05
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Shuiming1,SONG Fengshun1,GONG Xuemei1,YANG Zhenjiang2,HUANG Yongfeng2,ZHU Liwu1(1.Department of Horticulture, Anhui Agricultural University,Hefei 230036;2.Administration Committee, Protection District of Dangshansu Pear Germplasm Resources);Isolation of Genomic DNA of Dangshansu Pearand Optimization of Its RAPD Analysis Condition[J];安徽农业大学学报;2003-02
2 Zheng Jingsheng 1,2 Lü Bei 1 1 Hainan Provincial Key Laboratory of Crop Molecular Breeding,Sanya,572025 2 The Institute of Rice&wheat,Fujian Academy of Agricaltural Sciences,Fuzhou,350019;PCR Technique and its Practical Methods[J];分子植物育种;2003-03
3 WANG Zhuo-wei,YU Mao-de,LU Ceng(The Key Sericultural Laboratory of Agriculture Ministry,Southwest Agricultural University,Chongqing 400716,China);APPLICATION OF PVP IN TOTAL DNA EXTRACTION IN MULBERRY[J];西南农业大学学报;2001-01
4 DING Xiaodong 1,2 * ? ?&? ?LU Liuxin 1 ( 1 Deparment of Horticulture,Fujian Agricultural University , Fuzhou 350002) ( 2 Department of Horticulture,Northeast Agricultural University , Harbin 150030);STUDY ON GENOMIC DNA EXTRACTION FROM RECALCITRANT LITCHI[J];应用与环境生物学报;2000-02
5 Yi Ganjun; Huo Heqiang; Cai Changhe; Qiu Yanping; Huang Ziran; Chen Dacheng(1 Pomology Research Institute, Guangdong Academy of Agric. Sci., Guangzhou, 510640;2 Dept. of Sericulture, South China Agric. Univ.;3 Dept. of Horticulture, South China Agric. Uni;DNA Isolation Procedure for AFLP Amplification Reaction of Litchi[J];华南农业大学学报;1999-03
6 Chen Wenxin(College of Biological Sciences,China Agricultural Universyty,Beiji 100094);BACTERIAL PHYLOGENY[J];微生物学报;1998-03
7 (received May 23, 1997)Fang Xuanjun; Mou Tongmin; Lu Xinggui; Sun Mer (Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing 100081)(Hybrid Rice Eng. Tech. Research Center, Hubei Academy of Agricultural Sciences, Wuhan 430064)(D;Identification of Rice Hybrid Seed Using Allozyme Markers[J];高技术通讯;1997-08
8 Wang Yanfeng,Zhang Weizhang,Gao diming(Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021);Agronomic Character Analysis of Yardlong Bean Genetic Resources[J];中国蔬菜;1997-02
9 Dai Silan, ChenJunyu, Gao Rongfu, Ma Jiangsheng, and Li Wenbin (Beijing Forestry University , 100083; The Institute of Genetics, Academia Sinica, Beijing 100101);DNA Isolation Procedure for RAPD Amplification Reaction of Nine Dendranthema Species[J];园艺学报;1996-02
10 Zou Yu ping, Wang Xiao quan, Lei Yi ding, Pei Yan long and Zhang Zhi xian (Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Academia Sinica, Bejing 100093);ISOLATION AND CHARACTERIZATION OF TOTAL DNA FROM SEVERAL ENDANGERED SPECIES AND THEIR ALLIES[J];植物学报;1994-07
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