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《Journal of Northeast Agricultural University》 2008-02
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Study on transformation of bar gene into maize(Zea mays L.) by Agrobacterium tumefacien mediated

DI Hong1,LIU Zhaojun2,LU Cuihua1,WANG Zhenhua1(1.College of Agriculture,Northeast Agricultural University,Harbin 150030,China;2.Biotechnology Research Center,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)  
bar gene was introduced into 3 maize inbred lines by Agrobacterium tumefacien mediated.The results showed that 2 mg·L-1 2,4-D was good to embryogenic callus.The suitable duration of infection was 20 min.The addition of AS(200 μmol·L-1) in infection and co-culture medium enhanced the instant expression frequency of immature embryo.The herbicide concentration should be lower than lethal concentration,8 mg·L-1 was better.Moderate selection fitted for transformation,the best screening mode was to use hygromycin in the concentration of 8,6 and 2 mg·L-1 in induction medium,buds elongation medium and rooting medium respectively.Seven plants showed positive reaction by PCR analysis and were confirmed to resist basta.
【Fund】: 黑龙江省“十一五”攻关项目(GB07B104)
【CateGory Index】: S513
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Chinese Journal Full-text Database 1 Hits
1 XIAO Li-jie~(1*),WANG Li-yan~1,MIN Li~2 1.College of Life Science and Technology,Heilongjiang August First Land Reclamation University.Daqing 163319;2.Crop Research and Breeding Center of Land Reclamation,Harbin 150090;Callus Induction from Mature Embryos of Maize[J];农业科学与技术(英文版);2009-06
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1 Wang Guoying; Zhang Hong; Xie Youju ;Dai Jingru; Mi Jingjiu(Beijing Agricultural University, Beijing 100094)Li Taiyuan; Tian Yingchuan; Qiao Liya; Mang Keqiang(Institute of Microbiology,CAS,Beijing l00080);Transfer of Bt-toxin Protein Gene into Maize and the Resistance of Transgenic Plants to Corn Borer[J];JOURNAL OF AGRICULTURAL BIOTECHNOLOGY;1995-03
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1 YUN Lan et al (College of Ecology and Environmental Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010019);Effects of Hormone and Maturity on Callus Induction of Young Spike in Psathyrostachys juncea[J];Journal of Anhui Agricultural Sciences;2008-02
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5 JIA Xiu-ping1,WANG Han-ning1,ZHANG Jin-wen1,YUE Yun2(1.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China;2.Gansu Agriculture Technology Extend Station,Lanzhou 730020,China);Influence of 2,4-D and NAA on embryonic callus induction from different explants in mature seeds of the maize parent K12[J];Journal of Gansu Agricultural University;2008-06
6 ZENG Sheng-jian et al(College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009);Callus Induction and Plant Regeneration from Mature Seeds of Perennial Ryegrass[J];Journal of Anhui Agricultural Sciences;2009-35
7 YANG Ru-tao,WANG Han-ning,KONG Wei-ping,WEN Rui-ting,JIA Xiao-xia,MA Huai-yi(College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China);Establishment of plant regeneration system from immature embryos of maize (Zea mays L.) skeleton inbred lines[J];Journal of Gansu Agricultural University;2009-06
8 HUANG Min1,CHENG Shui-yuan2*(1.College of Life Sciences,Changjiang University,Jingzhou 434025,China;2.College of Horticulture and Forestry Sciences,Changjiang University,Jingzhou 434025,China);The Technology of Germplasm Resource Creation in Maize[J];Journal of Hebei Agricultural Sciences;2007-05
9 WEI Xiao-min1,2,WANG Peng1,JI Liang-yue2,HU Yan-min2(1.Department of Biology,Zhengzhou Animal Husbandry Engineering College,Zhengzhou 450011,China;2.College of Agronomy,Henan Agricultural University,Zhengzhou 450002,China);Selection of Salt-tolerant Callus Variants and Analyses of their Physiological Characters in Maize[J];Acta Agriculturae Boreali-Sinica;2007-03
10 ZHANG Gui-ling ZHANG Su-zhi RONG Ting-zhao (Maize Research Institute/Key Laboratory of Crop Genetic Resource and Improvement,Ministry of Education,Sichuan Agricultural University,Ya'an,Sichuan 625014);ANALYSIS OF FACTORS INFLUENCING REGENERATION RATE OF EMBRYOGENIC CALLUS FROM IMMATURE EMBRYO OF MAIZE[J];Journal of Nuclear Agricultural Sciences;2009-03
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