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《Journal of Northeast Agricultural University》 2008-06
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Study on the anatomical structures of blades and peroxide enzyme activity in the dwarf mutant soybean

ZHANG Da,WANG Yuying,CANG Jing,XU Jun,HAO Zaibin(College of Life Sciences,Northeast Agricultural University,Harbin 150030,China)  
The dwarf mutant Dongze11 was obtained from Dongnong42 soybean induced by NaN3 chemical,anatomical structures of blades were observed by paraffin-slice-up.The results showed that the thickness of blades,palisade tissue,spongy tissue and the thickness ratio of spongy to blade in the mutant were all smaller than those of the wild type;however,the thickness of palisade to blade and the thickness ratio of palisade tissue to spongy were all bigger,and the difference was bigger between the mutant and wild type when the dwarf phenomenon was more notable.In addition,the main vascular bundle number,vessel number,vessel diameter,area of transport water and area of phloem in main vein of blades were lower in the dwarf mutant.So the screen of dwarfing seedlings can be determined by the proportion of spongy and palisade thickness.The peroxide enzyme activities in the mutant and the wild type had an ascending trend.When mutants grew for 20 d,the peroxide enzyme activity was a little lower than that of the wild type,but there was no significant difference;while for 30,40 and 50 d,the peroxide enzyme activity of mutant became higher,with significant difference at 1% level.So it suggested that peroxide enzyme activity was related to the degree of dwarf in soybean.
【Fund】: 国家“863计划(”2003AA207060-5 2006AA100104);; 黑龙江省自然科学基金(AC2005-32);; 大豆生物学教育部重点实验室主任基金项目(SB06A01)
【CateGory Index】: S565.1
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