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《Journal of Northeast Agricultural University》 2008-07
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Study on antimicrobial mechanism and identification of biocontrol bacteria strain L1 against rice diseases in cold area

LI Yonggang1,HAO Zhongna2,YANG Minxiu1,LI Zheng1,ZHAO Xueying1(1.College of Agriculture,Northeast Agricultural University,Harbin 150030,China; 2.Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)  
Bacillus ssp.strain L1,one of the biocontrol bacteria strains,was screened and identified from rice rhizosphere.The antimicrobial activity of fermentation liquid to Pyricularia grisea and Rhizoctonia solani reached the highest after 5 days cultures,and inhibitory rate accounted for 74.57% and 83.23%,respectively.But it had not changed along with the time increasing.The filtrated Bacillus strain L1 was precipitated in grades with the ammonium sulfate to draw the antimicrobial proteins and crude proteins obtained.One crude protein drawn with ammonium sulfate at 50%-60% saturation showed strong antimicrobial activities to P.grisea and to R.solani at 60%-70%.Atimicrobial proteins to P.grisea and R.solani were different types.
【CateGory Index】: S435.111.41
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