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《Journal of Northeast Agricultural University》 2009-01
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Construction of vector for genetic transformation of Phytophthora sojae

SONG Chuanling, LI Lijun, WEN Jingzhi (College of Agriculture, Northeast Agricultural University, Harbin 150030, China )  
Construction of recombinant vector is the basis for genetic transformation. Recombinant vector used for genetic transformation of Phytophthora sojae was constructed with pCDNA3.1(-)/hygro as the backbone. Enhanced green fluorescent protein (EGFP) gene, the promoter and the terminator of the ham34 gene of Bremia lactucae were recombined to pCDNA3.1(-)/hygro by procedures such as digestion with restriction enzyme, dephosphatasing with CIPA, and lingation with T4 lingase, etc, then transformed into E.coli competent cell. The recombinant plasmid was identified by restriction endonuclease enzyme analysis. It provided a vector for further genetic transformation of Phytophthora sojae.
【Fund】: 公益性行业(农业)科研专项(nyhyzx07-053);; 黑龙江省“十一五”重点项目(GB06B105-1)
【CateGory Index】: S435.651
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