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《Journal of Northeast Agricultural University》 2009-01
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Effect of different Chinese herbs extracts on expression of β-casein of mouse mammary gland cells

GE Zengguang, TONG Huili, QIN Jun, LI Qingzhang, GAO Xuejun (Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China)  
Five Chinese herbs were selected to study the lactating function of mouse mammary gland epithelial cells including cowherb seed, pangolin scales, medulla tetrapanacis, radix rhapontici and dandelion which were used extensively. Effects of herbs on the expression of β-casein of mouse mammary gland epithelial cells cultured in vitro were detected, respectively. Real time quantitative PCR was used to detect the expression of β-casein mRNA level and capillary electrophoresis technique was used to analyze the protein expression. Stat5 was detected by laser scanning confocal influorescence microscopy. The results showed that medulla tetrapanacis, dandelion and radix rhapontici can significantly induce the expression of β -casein, while cowherb seed and pangolin scales both can significantly induce the expression of β-casein extremely. It implies that cowherb seed, pangolin scales and medulla tetrapanacis can raise lactating qualities through impacting the secretion of milk protins of mammary gland. However, the mechanism remains to be further investigated.
【Fund】: 东北农业大学创新团队发展计划资助(LXT005-1-2)
【CateGory Index】: S853.7
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