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《Journal of Northeast Agricultural University》 2009-02
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Differentiation of mating type and changes in fungicide resistance of Phytophthora infestans

XU Shengjun,GUO Rong,WANG Chen,ZHANG Xuanzhe(College of Agriculture,Northeast Agricultural University,Harbin 150030,China)  
In this research,the mating type of Phytophthora infestans isolates collected from various locations of Heilongjiang Province and Jilin Province were tested by standard strains(A1 and A2 isolates).The results showed that all isolates collected from 5 counties(cities) of Heilongjiang Province and Longjing city of Jilin Province were all A1 mating type,and no A2 isolate was detected.To screen the effective fungicide for potato late blight control,the resistance to different fungicides in vitro was determined.As for metalaxyl resistance,73.7%(42 isolates) were resistant,17.5%(10 isolates) were intermediate,and 8.8%(5 isolates) were sensitive.As for ethaboxam sensitivity,no isolates were able to grow at 1.0 μg·mL-1,and only one isolate could grow at 0.5 μg·mL-1.Basing on these results,the minimum inhibitory concentration(MIC) of ethaboxam for P.infestants should be 0.2-0.5 μg·mL-1.As for dimethomorph sensitivity,most isolates could be grown at 0.5 μg·mL-1 and two isolates could be grown at 1.0 μg·mL-1.Based on these results,the MIC of dimethomorph for P.infestants should be 0.5-1.0 μg·mL-1.In addition,no cross resistance was found for these three fungicides.
【Fund】: 中国博士后基金(20070410884);; 东北农业大学科学研究基金;; 教育部留学回国人员科研启动基金(210150)
【CateGory Index】: S435.32
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