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《Journal of Northeast Agricultural University》 2010-03
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Screening of rice varieties (lines) cold tolerance used SSR marker at booting stage in Heilongjiang Province

WANG Qi1,FENG Yanjiang1,ZENG Xiannan1,ZHANG Xiaoming2,BIAN Jingyang1(1.Crop Tillage and Cultivation Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;2.College of Agriculture,Northeast Agricultural University,Harbin 150030,China)  
This paper studied cold tolerance of 120 rice varieties(lines) planted in different regions of Heilongjiang Province at booting stage,cleared the differences of rice varieties(lines) cold tolerance.According to BSA method,selected each 12 DNA samples mixed equally by SSR markers at booting stage,which of cold tolerance of extreme values were the strongest and the weakest,composited resistant and susceptible pools,The screening of the main effect QTL linked with cold tolerance at booting stage SSR markers that had been published.By correlation analysis and consistent calculation,located RM229 and RM259 on rice chromosome 11 and 1,which were high common primers with Heilongjiang rice cold tolerance at booting stage.The similarity coefficient was 0.75 and 0.58,the accuracy was 83.3% and 75%.They can be used for screening of cold tolerance of rice varieties(lines) resources and corresponding generations in Heilongjiang Province.
【Fund】: 黑龙江省农业科技创新工程重点项目
【CateGory Index】: S511.032
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