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Derivation of H~+-ATPase-defective mutant from Lactobacillus delbru-eckii subsp.bulgaricus KLDS1.9201 and its stress response

HUO Guicheng1,2,CUI Lan1,2,LIU Fei1,2,3(1.Key laboratory of Dairy Science,Ministry of Education,Harbin 150030,China;School of Food Science,Northeast Agricultural University,Harbin 150030,China,3.Danisco(China) Co.,Ltd.,Kunshan Jiangsu 215300,China)  
One spontaneous neomycin mutant strain named KLDS1.9201-11 was obtained from Lactobacillus delbrueckii subsp.bulgaricus KLDS1.9201,which showed the reduced H+-ATPase activity.Compared with parent strain,the mutant strain had lower growth rate and acid yield ability.The acid tolerance of the two strains were compared by using three-hour-exposure process to acidic conditions with pH 3.0,results showed that the viable count of the parent strain and mutant strain reduced by 86.98% and 99.98%,separately,and the membrane-bound H+-ATPase activities of the parent strain and mutant strain reduced by 13.33% and 21.15%,respectively.The freeze-drying test showed that the viable count of the parent strain and mutant strain reduced by 76.71% and 4.88%,respectively,and the membrane-bound H+-ATPase activities of the parent strain reduced by 4.27%,while which of the mutant strain increased by 29.71%.In conclusion,compared to the parent strain,the mutant strain had higher sensitivity to acid and cold tolerance ability,which could be used to make the starter culture with low-postacidification ability.
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