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《Journal of Northeast Agricultural University》 2012-05
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Quality evaluation of food-grade lycopene microemulsion

WANG Jing1,LIU Ning1,2(1.Key Laboratory of Dairy Science,Ministry of Education,Harbin 150030,China;2.School of Food Science,Northeast Agricultural University,Harbin 150030,China;3.National Engineering Research Center for Dairy,Harbin 150086,China)  
The ingredients of lycopene microemulsion were screened by titration and pseudo-ternary phase diagram.A food-grade O/W microemulsion system was prepared with Tween 80/ethanol/(R)-(+)-limonene/water.The appearance of the microemulsion was observed under transmission electron microscope(TEM).The dynamic light scattering(DLS) was used to evaluate the particle diameters.The lycopene release rate in simulated intestinal in vitro fluid and the entrapment efficiency of lycopene were calculated by the measuring for lycopene with UV spectrophotometer.The results showed that the average particle size of this microemulsion was(43.0±2.35) nm.Because of little change within one month,the stability of microemulsion was well;the in vitro lycopene release rate was significantly greater than that of control fluid,and its cumulative release ratio reached 90% during 6 h;the average entrapment efficiency was above 97%;and after placing for one month,there were no delamination and demulsification,and the entrapment efficiency varied very little.
【Fund】: 国家“863”计划项目(2008AA10Z331)
【CateGory Index】: TS207
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