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《Journal of Northeast Agricultural University》 2012-05
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Technology of high cell-density of Lactobacillus rhamnosus(ATTC53103)

Chencheng1,Hanjianchun2(1.Heilongjiang Province Light Industrial Science Research Institute,Harbin 150010,China;2.School of Food Science,Northeast Agricultural University,150030,China)  
In this study,Lactobacillus rhamnosus(ATTC 53103) was used as the starting strain.Through single factor experiments and orthogonal design,the optimal basic culture medium was selected as the followings 12% skim milk powder,10% glucose,1% yeast extract,2% casein hydrolyzate,0.15% Tween 80.Cultivating condition in 10 L automatic fermentor,adding glucose from the concentration of 4-6 g·L-1 were best for Lactobacillus rhamnosus(ATTC 53103) fed-batch high-densityculture.As mentioned above,the strains were in the logarithmic phase during 20 h,the max viable count could reach 3.75×1010 cfu·mL-1 at the end of logarithmic phase.However,the maxviable count was only 3.8×109 cfu·mL-1 via automatic fermentor batch culture,the former increased by 10 times.
【Fund】: 黑龙江省教育厅普通高等学校青年学术骨干支持计划项目(1153G0006)
【CateGory Index】: TQ920.1
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【References】
Chinese Journal Full-text Database 1 Hits
1 WANG Zhi-ding;LIU Yan-qiu;LI Yue-fei;SUN De-jun;Department of Biomedicine, Institute of Frontier Medical Sciences, Jilin University;Dunhua Center for Disease Control and Prevention;;Optimization of Lactobacillus rhamnosus GG(LGG) high-density medium[J];Food Science and Technology;2013-11
【Citations】
Chinese Journal Full-text Database 3 Hits
1 WU Guangwei, HAN Jianchun(College of Food, Northeast Agricultural University, Harbin 150030, China);Study on bacteriocin produced by Lactobacillus rhamnosus(ATTC 53103)[J];Journal of Northeast Agricultural University;2009-08
2 NIU Aidi,HAN Jianchun(Food College,Northeast Agricultural University,Harbin 150030,China);Identification and study on bacteriocin-producing Lactobacillus screened from sauerkraut[J];Journal of Northeast Agricultural University;2009-10
3 AN Ying1,Wang Shi-bin2(1.Inner Mongolia Yili Industrial Group Co.,Ltd,technology center,Huhhot 010080;2.Valio Ltd Shanghai Rep Office,Shanghai 200021);Beneficial functions of probiotics LGG and application in dairy products[J];Food Science and Technology;2006-07
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 DONG Xi-wen et al (College of Land and Environmental Science,Shenyang Agricultural University,Shenyang,Liaoning 110161);Research on the In Vitro Bacteriostasis of Lactobacillus acidophilus[J];Journal of Anhui Agricultural Sciences;2008-07
2 LIU Cui-ping et al(Technical Center of Bright Dairy and Food Co.Ltd.of Shanghai,Shanghai 200436);Study on Purification and Analysis of Exopolysaccharides from Lactobacillus casei[J];Journal of Anhui Agricultural Sciences;2010-05
3 YANG Xin et al(State Key Laboratory of Biological Feed Engineering,Beijing Dabeinong Technology Group CO.LTD,Beijing 100193);Research Progress of Characteristics and Application of Lactobacillus delbrueckii[J];Journal of Anhui Agricultural Sciences;2011-08
4 XU Hui,WEI Pei-lian (Department of Biochemical Engineering,Zhejiang University of Science and Technology,Hangzhou 310012,China);Preparation of Bacillus subtilis Microecological Agent[J];Amino Acids & Biotic Resources;2008-02
5 HU Bo1,CHEN Xiang-nan2,LIANG Jin-zhong1 (1.School of Food Engineering,Harbin University of Commerce,Harbin 150076,China;2.College of Forestry,Northeast Forestry University,Harbin 150040,China);OPTIMIZATION OF CULTURE MEDIUM FOR LACTOBACILLUS.RHAMNOSUS L7-13 STRAIN[J];Journal of Beijing Technology and Business University(Natural Science Edition);2010-03
6 YU Xiao-hui reviewing,YAO Wen,ZHU Wei-yun checking(Laboratory of Gastrointestinal Microbiology,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China);Food allergy and the role of probiotics in its prevention and treatment[J];Parenteral & Enteral Nutrition;2007-05
7 WEI Ri-hua1,Guirong2,3,Tana2,3(1.College of Animal Science and Medicine,Inner Mongolia Agricultural University,Inner Mongolia Hohhot 010018,China;2.Grassland Research Institute of Chinese Academy of Agricultural Science,Inner Mongolia Hohhot 010010,China; 3.Key Laboratory of Grassland Resources and Ecology,Inner Mongolia Hohhot 010010,China);Isolation and identification of heterofermentative lactobacillus from forage[J];Pratacultural Science;2010-10
8 ZHANG Ying-hua1,2,HUO Gui-cheng1,2,GUO Ling1,2(1.Key Lab of Dairy Science,Ministry of Education,Harbin Heilongjiang 150030,PRC; 2.Food College,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC);Research advance of the freeze-drying technology of lactic acid bacteria[J];Journal of Northeast Agricultural University;2005-06
9 YANG Ping1,LIU Ning1,MA Yuyan2(1.Key Lab of Dairy Science,Ministry of Education,Food College,Northeast Agricultural University,Harbin 150030,China; 2.Cancer Research Institute,Harbin Medical University,Harbin 150081,China);Study on the inhibition of whole peptidoglycan of Bifidobacterium to experimental gastric cancer[J];Journal of Northeast Agricultural University;2008-03
10 CHU Guochao,ZHENG Xianzhe,ZHANG Wenhao,DING Ningye,LIU Chenghai(Engineering College,Northeast Agricultural University,Harbin 150030,China);Development of a continuous sampling and testing equipment by SPR biosensor[J];Journal of Northeast Agricultural University;2011-05
China Proceedings of conference Full-text Database 5 Hits
1 Xiao Zijun~1 Zhong Ruimin~1 Chen Huiyin~2 Yang Rude~2 (1.Shaoguan university. Shaoguan512005) (2.College of Food&Bioengineering, South China Univ.of Tech., Guangzhou510640);The Researcher and Development of L.plantarum[A];[C];2004
2 WEI Ri-hua~1,GUI Rong~(2*),TA Na~2 (1.College of Animal Science and Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;2.Grassland Research Institute of Chinese Academ of Agricultural Science,Hohhot 010010,China;3.Key Laboratory of Grassland Resources and Ecology,Hohhot 010018);Isolation and Identification of Heterofermentative Lactic Bacteria from Forage[A];[C];2009
3 Shan Chunqiao,Liu Qiuchen,Jiang Guotuo,Zhuang Guohong (Dalian Sanyi Bioengineering Research Institute,Sanyi Animal Medicine Co.,Ltd,Dalian,Liaoning,116036);Fermentation conditions and biological characteristic of Bacteriocin secreted by a strain of Lactobacillus Acidophilus[A];[C];2008
4 Wang xiaoli Wang yongshan Zhu yumei Zhu xiaocui Liu xiaojuan Zhou yu Wang ran (Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences Key Laboratory of Animal Diseases Diagnostic and Immunology,Ministry of Agriculture·National Center for Engineering Research of Veterinary Bio-products,Nanjing 210014,China);Identification and Studies on Biological Characteristics of 5 Strains of Lactic Acid Bacterias[A];[C];2010
5 WEI Ya-song1,LIU Guo-qing2,ZHAO Peng-wei1,WEN Jian-xin1(1.Qingdao Agricultural University College of animal science and technology,Shandong,Qingdao 266109,2.Jiaonan animal disease control center,Shandong Qingdao 266400);Separation and identification of mink feces Bifidobacterium[A];[C];2012
【Co-references】
Chinese Journal Full-text Database 6 Hits
1 XIE Wei-tian et al(Gungdong Ocean University,Zhanjiang,Guangdong 524088);Optimization of the Growth Medium for a Lactobacillus rhamnosus LT22 Strain[J];Journal of Anhui Agricultural Sciences;2012-12
2 ZHANG Wei-wei1,3,LI Yuan-yuan1,3,ZHOU Ming-feng1,3, HUANG Fan1,3,WANG Chang-gao1,3,LIN Jian-guo1,3,CAI Jun1,2,3(1 Key Laboratory of Fermentation Engineering(Hubei University of Technology), Ministry of Education,Hubei Wuhan 430068;2 Hubei Provincial Key Laboratory of Industrial Microbiology,Hubei Wuhan 430068;3 College of Bioengineering,Hubei University of Technology,Hubei Wuhan 430068;4 Hubei Pharmacy School,Hubei Wuhan 430068,China);Optimization of Medium Components of the Production of L-lactic Acid by Lactobacillus Rhamnosus CLRI[J];Guangzhou Chemical Industry;2012-07
3 ZHU Chengliang,YE Xuefei,ZHU Shixiang,HE Guoqing(Department of food Science and Nutrition,Zhejiang University,Hangzhou 310029,China);Study on High Cell Density Culture of Lactobacillus acidophilus[J];Bulletin of Science and Technology;2009-02
4 Shi Tongling,Lu Ye,Liang Jinzhong*(Food College of Harbin University of Commerce,Harbin of Heilongjiang 150076,China);Optimization of High-density Growth Medium of Lactobacillus acidophilus Strain HUC-La-0812[J];Journal of Dairy Science and Technology;2009-06
5 Li Dan,Zhao Xinhuai(Key Laboratory of Dairy Science of Ministry of Education,Northeast Agricultural University,Harbin 150030,China);Chemical De-stressing Effect on High-density Fermentation of Lactobacillus acidophilus[J];Food and Fermentation Industries;2007-03
6 AN Ying1,Wang Shi-bin2(1.Inner Mongolia Yili Industrial Group Co.,Ltd,technology center,Huhhot 010080;2.Valio Ltd Shanghai Rep Office,Shanghai 200021);Beneficial functions of probiotics LGG and application in dairy products[J];Food Science and Technology;2006-07
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 Xie Mingyong;Xiong Tao;Guan Qianqian;State Key Laboratory of Food Science and Technology, Nanchang University;;Research Progress on the Key Techniques of the Fruit and Vegetable Products Fermented by Probiotics[J];Journal of Chinese Institute of Food Science and Technology;2014-10
【Secondary Citations】
Chinese Journal Full-text Database 9 Hits
1 Xu Xinjun;Therapeutic Effects of L. acidophillus and Its Application[J];China Dairy Industry;1990-05
2 Li Pinglan Zhang Chi Dept.of Food Science,China Agricultural University;Characterization and Application of Nisin[J];China Dairy Industry;1997-04
3 (Department of Food Science of Agricultural College of Yangzhou University)Xie Jizhi; Gu Ruixia;Effects of Continuous Transfers of Mixed Yoghurt Culture on the Stability of Mother Culture[J];CHINA DAIRY INDUSTRY;1995-01
4 Liu Yufeng; Wang Jinying (Institute of Applied Microblolgy,Heilongjiang Academy of Science, Harbin);Preparation of Freeze Dried Direct-to-Vat Cultures for Yoghurt Production[J];CHINA DAIRY INDUSTRY;1995-06
5 Qiao Fadong Wu Xiufang Nan Qingxian Lan Fengying(He Nan Vocational and Technical Teachers College)(China Agricultural University) ;Present Status of Concentrated Lactic Acid Bacteria Starter Culture[J];CHINA DAIRY INDUSTRY;1998-01
6 Zhang Lanwei Liu Wei (Northeast Agricultural University) (Harbin Dairy Products Factory) (Heilongjiang Dairy Group Company);Influence of Added Nutriment on Growth of Streptococcus Thermophilus and Lactobacillus Bulgaricus[J];CHINA DAIRY INDUSTRY;1999-01
7 Zhu Xiaoqiao Liu Xongxun (Food & Bioengineering College, South China University of Technology, Guangzhou,510641);A Natural Preservative with Potentialities-Nisin[J];Food and Fermentation Industries;2001-04
8 Zhang Lanwei She Meiyan Zhang Xiaoguang;Influence of pH, Temperature and Contanue Inoculum on Mixed Cultures of Streptococcus Thermophilus CH9-9 and Lactobacillus Bulgaricus CH9-3[J];Food and Machinery;1996-05
9 Guo qingquanZhang LanweiFeng ZhenLin Shuying;The Application of Nisin in Yogurt[J];Food and Machinery;2001-04
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