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《Journal of Northeast Agricultural University》 2012-05
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Technology of high cell-density of Lactobacillus rhamnosus(ATTC53103)

Chencheng1,Hanjianchun2(1.Heilongjiang Province Light Industrial Science Research Institute,Harbin 150010,China;2.School of Food Science,Northeast Agricultural University,150030,China)  
In this study,Lactobacillus rhamnosus(ATTC 53103) was used as the starting strain.Through single factor experiments and orthogonal design,the optimal basic culture medium was selected as the followings 12% skim milk powder,10% glucose,1% yeast extract,2% casein hydrolyzate,0.15% Tween 80.Cultivating condition in 10 L automatic fermentor,adding glucose from the concentration of 4-6 g·L-1 were best for Lactobacillus rhamnosus(ATTC 53103) fed-batch high-densityculture.As mentioned above,the strains were in the logarithmic phase during 20 h,the max viable count could reach 3.75×1010 cfu·mL-1 at the end of logarithmic phase.However,the maxviable count was only 3.8×109 cfu·mL-1 via automatic fermentor batch culture,the former increased by 10 times.
【Fund】: 黑龙江省教育厅普通高等学校青年学术骨干支持计划项目(1153G0006)
【CateGory Index】: TQ920.1
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