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《Journal of Northeast Agricultural University》 2013-10
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Agrobacterium-mediated transformation of potato with EPSPS gene

LU Cuihua;WANG Shi;ZHANG Lili;CAO Shiliang;JIANG Lili;ZHANG Huan;PAN Yingxue;LIN Zhongping;School of Agriculture, Northeast Agricultural University;Institute of Maize Research, Heilongjiang Academy of Agricultural Sciences;School of Agriculture, Heilongjiang Bayi Agricultural University;School of Life Sciences, Peking University;  
In this study, EPSPS gene was transferred into the potato(Solanum tuberosum L.)cultivars, 'Favorite' by Agrobacterium- mediated transformation, and influence factors of genetic transformation were optimized. Microtuber discs were used as explants.Resistant plants were analyzed by PCR detection, of which 17 plants were positive. By RT-PCR test,six strains of 'Favorite' were positive;EPSPS gene could be transcribed nomally. And these resistant plants were analyzed by Southern blot,of which five plants had a positive amplification. Southern blot showed that single copies of the EPSPS gene were inserted into the genome of the transgenic plants. By the result of glyphosate resistant experiment, the transgenic materials showed resistance to glyphosate.
【Fund】: 国家高技术研究发展计划项目“863计划”(2009AA10Z103);; 黑龙江省科技攻关计划项目(GZ11B103)
【CateGory Index】: S532
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Yao Yingqiu1 Chen Xing2 Wang Pu1 Liang Yan2 Yan Mei2 Xiao Bai2(1College of Life Science,Capital Normal University,Beijing 100037;2Beijing Chaoyang Hospital Affiliate of Capital of Medical Sciences,Beijing 100020);Improved Methods for Isolation of High Quality Total RNA from Glycine max Leaves[J];Biotechnology Bulletin;2008-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Quan - hua, WANG Xiu - feng (College of Horticulture,Shandong Agricultural University,Taian 271018, China) Key words: tomato, transgene, resistance breeding;RECONT PROGRESSES OF TOMATO BREEDING FOR DISEASE RESISTANCE BY TRANSGENIC TECHNIQUES[J];Journal of Shandong Agricultural University;2003-04
2 WU Li min, ZHENG You liang, WEI Yu ming, WU wei, YAN Ze hong, ZHANG Zhi qing (Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, Sichuan, China);Advances in Wheat Genetic Transformation[J];Journal of Sichuan Agricultural University;2003-02
3 Lin Hongsheng 1,2) Hua Zhihua 2) Lu Dezhao 2) Gao Zhenyu 2) Yan Meixian 2) Qian Qian 2) Huang Danian 2) ( 1) Hansan Normal College,Chaozhou 521041; 2) Key Laboratory for Rice Biology of Ministry of Agri;COMPARATIVE STUDY ON SELECTIVE MARKER GENES USED IN RICE TRANSFORMATION BY PARTICLE BOMBARDMENT[J];Acta Agriculturae Shanghai;2001-03
4 WANG Xiu-jun1,2 LANG Zhi-hong2 SHAN An-shan1 HUANG Da-fang2(1 College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China)(2 Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China);Advances in Mechanism of Herbicide in Inhibiting Amino Acid Biosynthesis and Herbicide-tolerant Transgenic Plants[J];China Biotechnology;2008-02
5 Peng Honghai Luo Yuping Li Siguang(College of Life Science,Nanchang University,Nanchang 330047);Antibacterial Peptide and Its Progress in Advance on Transgenic Plant[J];Biotechnology Bulletin;2007-01
6 Ma Hong(Hebei Institute of Biology,Shijiazhuang 050081);Progress on Control of Potato Soft Rot in China[J];Biotechnology Bulletin;2007-01
7 Fan Yali1,2 Liu Chunlin2 Ruan Ying1 Deng Huifen1,2 Li Jin1,2(1College of Bio-Science and Technology,Hunan Agriculture University,Changsha 410128;2CropGene Engineering Key Laboratory of Hunan Province,Changsha 410128);Advance on Resisting-disease Gene Engineering in Potato Plants[J];Biotechnology Bulletin;2007-03
8 He Fuxia1,2 Lang Zhihong2 Lu Wei2 Lin Min2 Zhang Jie3 Huang Dafang2(1College of Life Science,Northeast Agricultural University,Harbin 150030;2Biotechnology Research Institute,Chinese Academy of Agricultural Science,Beijing 100081;3Key Laboratory of Biology of Plant Disease and Insect Pest,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100094);The Establishment of Maize Transformation System with a Glyphosate-tolerant 2mG2-epsps Gene as a Selectable Marker[J];Biotechnology Bulletin;2008-05
9 Zhang Chenming Hu Zongli Chen Guoping(Bioengineering College of Chongqing University,Chongqing 400044);Transformation Mediated by Agrobacterium tumefaciens in Potato Plants and Gene Engineering for Virus Resistance[J];Biotechnology Bulletin;2008-06
10 Zhang Xin1.2 Miao Xiangyang1 Yin Xunhe2 Ma Yanfang2 Qu Zhaojie1,2 Zhang Qiuting1,3(1Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100094;2Animal Science and Technology,Shandong Agricultural University,Taian 271018;3College of Life Science,Northeast University of Forestry,Harbin 150040);Progress of Antibacterial Peptides in Transgenic Plants and Animals[J];Biotechnology Bulletin;2009-02
China Proceedings of conference Full-text Database 2 Hits
1 ZHUO,Chunliu,CAI Jiongliang,FANG Junyang,ZHOU Ming,GUO Zhenfei (Biotechnology Laboratory for Turfgrass and Forages,Department of Plant Science,College of Life Sciences,South China Agricultural University,Guangzhou 510642:China.);Early light induced protein(ELIP) gene from Medicago falcata confers resistance to abiotic stresses[A];[C];2010
2 HU Cheng XIE Cong-hua TIAN Zhen-dong LIU Jun (Huazhong Agricultural University,WUHAN 430070);ADVANCES OF MOLECULAR BIOLOGY ON POTATO DISEASE RESISTANCE[A];[C];2002
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 SHAO Bi-ying,CHEN Wen-bing,YANG Jie (Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350003, China);Multiplex PCR Detection of Genetically Modified Components in Potato and Its Products[J];Food Science;2006-01
2 ZHU Sha1,ZHANG Chun-hui2,LI Xian-ju3(1.Medical College,Zhengzhou University,450001 Zhengzhou,China;2.Zhengzhou College of Animal Husbandry Engineering,450011 Henan;3.Technical Secondary School of Neihuang,456300 Hennan,China);Study from genomics to functional proteomics[J];Journal of Biology;2007-01
3 LU Xin,ZHAO Bing-zi,ZHANG Jia-bao,DENG Jian-cai,LI Li-ping,XIN Xiu-li (Fengqiu agro-ecological experiment station,State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Science,Nanjing 210008,China);Property and Environmental Behavior of Herbicide Glyphosate[J];Chinese Journal of Soil Science;2005-05
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 WANG Fang1,2, ZHANG Bin2, SHAN Lei2, BI Yu-ping2, XU Ze-yong3(1. School of Life Sciences, Shandong Normal University, Jinan 250014,China; 2. Plant Biology Technology Research Center, SAAS, Jinan 250100,China; 3. Oil Crops Research Institute ,CAAS,Wuhan 430062,China);An Improved Method for RNA Isolation from Peanut Seeds[J];Journal of Peanut Science;2002-04
2 ZHOU Zhuang-Zhi, ZHOU Yong-Gang, HE Chao-Zu, MANG Ke-Qiang, TIAN Ying-Chuan * (State Key Laboratory of Plant Genomics, Institute of Microbiology, The Chinese Academy of Sciences,Beijing 100080, China);Expression of cry3A and vhb Genes in Transgenic Potato Plants[J];Progress In Biochemistry and Biophysics;2004-08
3 ZHAO Cun\|You  \ YUAN Zheng\|Qiang\ QIN Hong\|Min\ TIAN Ying\|Chuan * (Plant Biotechnology Laboratory,Institute of Microbiology,Chinese Academy of Sciences,Beijing\ 100080,China);Studies on Transgenic Tobacco Plants Expressing Two Kinds of Insect Resistant Genes[J];Chinese Journal of Biotechnology;2001-03
4 XIE Long_Xu 1 XU Pei_Lin 1* NIE Yan_Fang 1 TIAN Ying_Chuan 2* 1(The Key Laboratory of Gene Engineering of Ministry of Education, Zhongshan University, Guangzhou 510275,China) 2(Institue of Microbiology, Chinese Academy of Science, Beijing 100080,China);Construction of a Vector Conferring Herbicide and Pest Resistance in Tobacco Plant[J];Chinese Journal of Biotechnology;2003-05
5 Yao Yingqiu1 Chen Xing2 Wang Pu1 Liang Yan2 Yan Mei2 Xiao Bai2(1College of Life Science,Capital Normal University,Beijing 100037;2Beijing Chaoyang Hospital Affiliate of Capital of Medical Sciences,Beijing 100020);Improved Methods for Isolation of High Quality Total RNA from Glycine max Leaves[J];Biotechnology Bulletin;2008-01
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