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《JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY》 1999-02
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A PRELIMINARY STUDY ON USING THE SEEDLING SOAKING METHOD TO INDUCE THE GENETIC CHARACTERS IN FLUE-CURED TOBACCO

Zhu Shengwei,Xu Zhong,Zhu Xiangchun,Shi Zhiwen (Northeast Agricultural University,Harbin 150030,PRC)  
Be aimed at cultivating disease-resistant Varieties.In this experiment,seedling soaking were used to study the way of exogenous DNA introduction into flue-cured tobacco with Cv87as the donor and Nc89 as the receptor.By means of phenotype,disease-resistance and isozyme test,selecting diseased-resistant variation materials.The result showed that exogenous DNA introduction could effectively transfer the haracter of plant heightplant typeleaf areagrowth period and disease resistance,meanwhile obtained higher transformation ratio(7.10%).Its operation rule was simple,and it was proved an effective method.
【CateGory Index】: S572.03
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