Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Northeast Forestry University》 2002-02
Add to Favorite Get Latest Update

The Main Points and Principles of Isolating Total RNA from Ligneous Plant Tissues

Wang Yucheng, Yang Chuanping, Jiang Jing(Northeast Forestry University, Harbin 150040, P. R. China)  
According to the knowledge and experiences acquired in isolating total RNA from ligneous plant tissues, the authors probe in and discuss the problems that often occur in isolating total RNA from ligneous plant tissues, such as low RNA output, RNA degrading, the interference of hydroxybenzenes, DNA, amylose, protein and other impurities, then bring forward the resolvable ways of these problems in particular. Meanwhile, sort the RNA isolating ways in common use into some categories, and also discuss their principles and evaluate their strongpoints and inferiors.
【Fund】: 国家“973”项目 (柽柳耐盐基因克隆 )部分内容
【CateGory Index】: Q943.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 10 Hits
1 LIU Xiao-ju1,HONG Hai-bo2,LI Min1,LIU Yuan1,YANG Ke-qiang 1(1.College of Forestry,Shandong Agricultural University,Taian 271018,China;2.Haidong Agricultural Extension Center in Qinghai,Haidong 810600,China);An Improved CTAB Method for Extracting Total RNA from Walnut[J];Shandong Agricultural Sciences;2008-01
2 YI Qing-ping et al (Department of Bio-engineering,Jingchu College of Technology,Jingmen,Hubei 448200);Advances of Genome DNA Extraction and Purification in Plants[J];Journal of Anhui Agricultural Sciences;2007-25
3 LI Na et al(Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants,Mininstry of Education,Beijing Forestry University,Beijing 100083);Comparative Study on Methods of RNA Isolation from Phloem of Anthocephalus chinensis[J];Journal of Anhui Agricultural Sciences;2008-11
4 Geng Hongwei1) Wang Chun1) Zhou Yijun2) Zhang Genfa1)(1)College of Life Science,Beijing Normal University,100875,Beijing,China;2)College of Life and Environmental Sciences,Central University for Nationalities,100081,Beijing,China);AN IMPROVED METHOD FOR EXTRACTION OF TOTAL RNA FROM MATURE LEAVES OF JOJOBA AND SUBTRACTION cDNA LIBRARY CONSTRUCTION[J];Journal of Beijing Normal University(Natural Science);2007-01
5 Yan Enwei1,Liu Hua2,Wang Yucheng3(1.Heilongjiang Institute of Forest By-product and Speciality,Key laboratory of Forest By-product and Speciality,Heilongjiang Mudanjiang 157011;2.Daxinganling Forestry management Burean of Songling;3.College of Forestry,Northeast Forestry University);A Method for Isolation of Total RNA from Schisandra chinesis[J];Forest By-Product and Speciality in China;2009-04
6 Zhan Yaguang, Zeng Fansuo(Northeast Forestry University, Harbin 150040, P. R. China);A Method for DNA Extraction from Mature Birch Leaves Rich in Polysaccharide[J];Journal of Northeast Forestry University;2005-03
7 Liu Ying,Wang Li,Jiang Yang,Li Sibin,Qi Changqing,Zu Yuangang(Key Laboratory of Forest Plant Ecology of Ministry of Education,Northeast Forestry University,Harbin 150040,P.R.China);A Modification Method of Extracting Total RNA in Compositae Different Tissues[J];Journal of Northeast Forestry University;2008-01
8 Fu Chang Wang Yu-Ying Dai Hong-Jie (Life and Environment College, Harbin Normal University, Harbin, 150080);ANALYSIS OF RNA LATION FROM DIFFERENT SOYBEAN SRGAS[J];Soybean Science;2004-04
9 Li Dongdong~* Fan Yongmei Department of Biotechnology,College of Science and Technology,Hainan University,Haikou,570228;Extraction and Quality Analysis of Total RNA from Coconut Pulp Tissue[J];Molecular Plant Breeding;2007-06
10 LUAN Dong-dong1, SHI Qing-hua2, CHEN Hong1, DONG Yu-zhi1,2 (1.College of Forestry,Xinjiang Agriculture University,Urumqi 830052,China;2.Key Laboratory of Agricultural Biotechnology,Agriculture University,Urumqi 830052,China);Comparison of Different Methods for RNA Extraction from Capparis spinosa[J];Arid Zone Research;2009-03
Chinese Journal Full-text Database 10 Hits
1 ZHENG Zhao-ying1, SUN Xiao-lei2, LIU Shi-qi1, GAO Jun-feng2(1Horticulture College, Shandong Agricultural University, Taian 271018, China;2Vegetable Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China);Study on ethylene distribution in cucumber plant and its year-round dynamics[J];Shandong Agricultural Sciences;2003-04
2 CHEN Yi-yin,CHEN Yu-zhen(College of Biological Sciences and Biotechnology,Beijing 100083,China);Cold Acclimation-Induced Changes in Freezing Resistance,the Contents of Soluble Protein and Proline and Antioxidant Enzyme Activities in Populus euphratica Calli[J];Shandong Agricultural Sciences;2007-03
3 Quan Xianqing (Department of Biology, Linyi Normal University, Linyi,Shandong 276005);No-enzymatic Mechanism of Scavenging Reactive Oxygen Species of Euhalophytes[J];Journal of Anhui Agricultural Sciences;2003-03
4 JIANG Gui-bo et al(Jieyang Vocational and Technical College,Jieyang,Guangdong 522000);Damages and Control Measures of Invasive Plants[J];Journal of Anhui Agricultural Sciences;2006-02
5 LI Zi-feng et al (Laiyang Agricultural University Landscape Architecture and Art,Qingdao,Shandong 266109);Research Advance in Chinese Peony Tissue Culture[J];Journal of Anhui Agricultural Sciences;2006-13
6 YANG Gu-liang et al(Huanggang Normal College,Huanggang,Hubei 438000);Comparison of RNA Extraction from Ginkgo biloba L.Leaf and RT-PCR[J];Journal of Anhui Agricultural Sciences;2007-12
7 ZHANG Kai tai* , LI Gang, WU De chang, et al. Institute of Radiation Medicine, Beijing 100850, P.R.China;A combination of biotin labeled screeing of cDNA library and RACE technique to identify a novel human gene HRNT-1[J];CHINESE JOURNAL OF CANCER;2000-09
8 WU Jun-chao, JIANG Ying (Biochemistry Department of Medical College of Suzhou University, Suzhou 215007);Development of Rapid Amplification of cDNA Ends[J];Amino Acids & Biotic Resources;2003-01
9 Zhao Yunyun;Tian Ruling;Liu Jieping(Department of Biology, Capital Normal University,Beijing 10037);Study on Amino Acid Composition and Content in Bark and Leaves of Male and Female Eucommia ulmoides Oliv[J];AMINO ACIDS & BIOTIC RESOURCES;1996-02
10 GU Shou-qin1, XIE Ling-jun2, FAN Yong-shan3 (1.Department of Biology, Baoding Teachers College, Baoding 071000, China;2.Th e Dingzhou branch school of Baoding Teachers College, Dingzhou 073000, China;3. Department of Biology, Tangshan Teachers College, Tangshan 063000, China);Common Methods of Total RNA Isolation from Plant Tissues and Its Optimization Strategies[J];Journal of Baoding Teachers College;2005-02
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 MA Li(Department of Biology,Zaozhuang College,Zaozhuang 277160,China);Two Fast Methods for Total RNA Extraction from Root Tubers of Sweet Potato[J];Shandong Agricultural Sciences;2008-06
2 CHEN Chang-bao,ZHU Shu-hua,ZHOU Jie(College of Chemistry and Material Science,Shandong Agricultural University,Taian 271018,China);Improved CTAB Method for Total RNA Extraction of Mature Feicheng Peach Fruit[J];Shandong Agricultural Sciences;2009-05
3 YANG Gu-liang et al(Huanggang Normal College,Huanggang,Hubei 438000);Comparison of RNA Extraction from Ginkgo biloba L.Leaf and RT-PCR[J];Journal of Anhui Agricultural Sciences;2007-12
4 JIANG Chang-hua et al (Institute of Genetics, State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai 200433);Methods Study on Extracting DNA and Total RNA from China Rose[J];Journal of Anhui Agricultural Sciences;2008-21
5 TANG Li-bo et al(Department of Biology Engineering,Zunyi Medical College Zhuhai Campus,Zhuhai,Guangdong 519043);Genetic Diversity Analysis and Optimization of RAPD Reaction System of Michelia[J];Journal of Anhui Agricultural Sciences;2009-01
6 LIN Zhi-hua et al(College of Life Sciences,Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225);Study on Extraction of Actinidia Genomic DNA[J];Journal of Anhui Agricultural Sciences;2009-03
7 SI Bin-bin et al(College of Life Science and Engineering,Northern University for Nationalities,Yinchuan,Ningxia 750021);Comparison of Different Extraction Methods of Genomic DNA from the Leaves of Lycium barbarum L.[J];Journal of Anhui Agricultural Sciences;2009-15
8 FAN Yong-shan1,GU Shou-qin2,LI li1(1.Department of Biology,Tangshan Teachers College,Tangshan 063000,China;2.Department of Biology,Baoding Teachers College,Baoding 071000,China);Genomic DNA Extraction from Phalaenopsis hybrid by CTAB Method[J];Journal of Baoding Teachers College;2005-04
9 WANG Yong-cheng,GONG Zhen-hui,LI Da-wei(College of Horticulture,Northwest Agricultural and Forestry University,Yangling Shanxi 712100,China);Cloning of the Genes Resistant to Phytophthora Capsici in Pepper(Capsicum annuum L) by mRNA Differential Display Technique[J];Northern Horticulture;2008-12
10 LI Hai-xia;ZHANG Zhi-yi;ZHANG Qian;AN Xin-min;LI Yan;LIU Ting-ting. National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,Beijing Forestry University,100083,P.R.China.;Transformation of tobaccos with a TIR-NBS-LRR gene isolated from Populus tomentosa Carr[J];Journal of Beijing Forestry University;2009-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved