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《Journal of Northeast Forestry University》 2003-01
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RAPD Analysis of Intraspecific Polymorphism in Vitis amurensis

Wang Jun, Ge Yuxiang, Bao Yihong, Chen Xiaoqiang(Northeast Forestry University, Harbin 150040, P. R. China); He Puchao(Northwest Agriculture University)//Journal of Northeast Forestry University.-2003,31(1).-19~21  
random primers were screening out from 7 germplasm of V. amurensis samples whose flower styles were not the same. Among these primers, 12 primers whose bands style was clean and stable were selected for applying . The genomic DNA fingerprinting was all acquired in 7 samples by using 12 primers, and the polymorphism was different for different primers. The bands that the different primer amplified was different and the band number ranged from 2 to 9.11 of 12 primers in 7 samples could produce 1~5 same bands. 26 of 74 bands were monomorphic bands. The phenomenon suggested the similarity and variability of genetic background of V. amurensis . The genetic similarity coefficient of 7 samples was classified by UPGMA method. The result was similar to morphology taxonomy, which suggested that RAPD was effective in identifying gene type.
【CateGory Index】: S663.1
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