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《Journal of Northeast Forestry University》 2005-03
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A Method for DNA Extraction from Mature Birch Leaves Rich in Polysaccharide

Zhan Yaguang, Zeng Fansuo(Northeast Forestry University, Harbin 150040, P. R. China)  
Genomic DNA was extracted from mature birch leaves which is rich in polysaccharide by means of five different methods of SDS, improved SDS, routine CTAB, CTAB with high salt and improved CTAB. The purity and quality of DNA obtained by these methods were compared, and the quality was evaluated by agarose gel electrophoresis. The result showed that DNA sample obtained by improved CTAB method showed the highest purity and quality. This method was suitable for genomic DNA extraction from mature birch leaves and could satisfy the need for further analysis.
【Fund】: 黑龙江省自然科学基金(C0212)资助。
【CateGory Index】: Q943.2
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