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《Journal of Northeast Forestry University》 2005-03
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A Method for DNA Extraction from Mature Birch Leaves Rich in Polysaccharide

Zhan Yaguang, Zeng Fansuo(Northeast Forestry University, Harbin 150040, P. R. China)  
Genomic DNA was extracted from mature birch leaves which is rich in polysaccharide by means of five different methods of SDS, improved SDS, routine CTAB, CTAB with high salt and improved CTAB. The purity and quality of DNA obtained by these methods were compared, and the quality was evaluated by agarose gel electrophoresis. The result showed that DNA sample obtained by improved CTAB method showed the highest purity and quality. This method was suitable for genomic DNA extraction from mature birch leaves and could satisfy the need for further analysis.
【Fund】: 黑龙江省自然科学基金(C0212)资助。
【CateGory Index】: Q943.2
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Chinese Journal Full-text Database 10 Hits
1 LIN Zhi-hua et al(College of Life Sciences,Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225);Study on Extraction of Actinidia Genomic DNA[J];Journal of Anhui Agricultural Sciences;2009-03
2 SI Bin-bin et al(College of Life Science and Engineering,Northern University for Nationalities,Yinchuan,Ningxia 750021);Comparison of Different Extraction Methods of Genomic DNA from the Leaves of Lycium barbarum L.[J];Journal of Anhui Agricultural Sciences;2009-15
3 FAN Yong-shan1,GU Shou-qin2,LI li1(1.Department of Biology,Tangshan Teachers College,Tangshan 063000,China;2.Department of Biology,Baoding Teachers College,Baoding 071000,China);Genomic DNA Extraction from Phalaenopsis hybrid by CTAB Method[J];Journal of Baoding Teachers College;2005-04
4 Zhang Miaobin Pan Lijing Fan Ganqun Chen Jimin Cheng Ping Zhuhai Agricultural Research Centre, Zhuhai, 519070;Study on DNA Isolation from Polysaccharides-rich Transgenic Dendrobium[J];Molecular Plant Breeding;2009-01
5 SONG Xiao-min1,LI Bao-guang1,HUANG Dong-hua1,XIE Qi-xin1,NI Guo-ping1,ZHANG Chun2(1.Institute of Vegetable and Flower,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China; 2.Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China);Separation Strategy of Functional Gene of Plants Based on DNA Molecular Marker[J];Acta Agriculturae Jiangxi;2008-05
6 ZHAN Ya-Guang~ 1* SU Tao~1 HAN Mei~2 SUN Dong~1(1.College of Life Science, Northeast Forestry University, Harbin 150040) (2.Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040);A multiplex polymerase chain reaction method for rapid detection of foreign genes in transgenic birch (Betula platyphylla)[J];Bulletin of Botanical Research;2006-04
7 ZHOU Ze-Gang FANG Yan-Ming WANG Biao(Nanjing Forestry University,Nanjing 210037);Genomic DNA Extraction and Optimization of RAPD Analytic Conditions of Fagus engleriana[J];Bulletin of Botanical Research;2008-06
8 SHI Jiang-Tao LI Jian(Key Laboratory of Bio-based Material Science and Technology,Ministry of Education,Northeast Forestry University,Harbin 150040);Purification and Identification of Populus ussuriensis Genomic DNA[J];Bulletin of Botanical Research;2009-02
9 Zhao Lei et al(1College of Life Science,Hebei University,Baoding,071002,china;2Hebei University library;3Baoding No.7 middle school);A Modified Method for Isolating Genomic DNA from Phytophthora infestans[J];Anhui Agricultural Science Bulletin;2009-10
10 DU Jin-zi1,CHEN Li-mei2,HUANG Rong-shao1,LI Hua1,HUANG Mian3(1.Agriculture College of Guangxi University,Guangxi Nanning 530005,China;2.Biotechnology Research Center of Kunming University of Science and Technology,Yunnan Kunming 650224,China;3.Management Office of Damingshan National Nature Reserve,Guangxi Wuming 530115,China);Study on Pyrrosia Genomic DNA Extraction by Modified SDS Method[J];Southwest China Journal of Agricultural Sciences;2009-03
Chinese Journal Full-text Database 5 Hits
1 Wang Yucheng, Yang Chuanping, Jiang Jing(Northeast Forestry University, Harbin 150040, P. R. China);The Main Points and Principles of Isolating Total RNA from Ligneous Plant Tissues[J];Journal of Northeast Forestry University;2002-02
2 YAO Qing-rong1, 2, ZHAO Chang-zeng1,WANG Wen-quan2 (1. Department of Horticulture, Gansu Agricultural University,Gansu, Lanzhou 730070;2. National Key Biotechnology Laboratory for Tropical Crops, CATAS, Haikou 571101, China);Extraction of genomic DNA and optimization of SSR amplified condition in litchi[J];Journal of Gansu Agricultural University;2004-02
3 LIU Dianlin,YANG Ruihuan,HA Yujie(Tianjin Cucumber Research Institute,Tianjin300192,China);Genomic DNA Extraction and RAPD Analysis of Cucumber[J];Acta Agriculturae Boreall—sinica;2002-04
4 Ying Tongming Han Zhengmin Huang Minren (College of Forest Resources and Environment Nanjing Forestry University Nanjing 210037);DNA EXTRACTING METHODS FROM VEGETATIVE TISSUE OF CONIFER[J];JOURNAL OF NANJING FORESTRY UNVERSITY;1998-04
5 ZHOU Bo,ZHANG Yang,LI Yu-hua Research Institute of Flower Biotechnology,Northest Forestry University,Harbin150040,China;Improvement of extraction method of total RNA from strawberry fruit with abundant polymeric carbohydrates[J];Letters in Biotechnology;2004-01
Chinese Journal Full-text Database 10 Hits
1 Liu Xiaoying Zheng Shilian Deng Chuanjun Sun Li (Department of Biology. Anhui University Hefei 230039) Song Degui (Hefei Biochemical Pharmaceutical Factory 230031);Research on the purification of Gyp and its properties[J];JOURNAL OF ANHUI UNIVERSITY(NATURAL SCIENCES);1996-04
2 WANG Jia et al(College of Landscape Architecture,Beijing Forestry University,Beijing 100083);Study on Optimization for ISSR Reaction System of Peony with Orthogonal Design[J];Journal of Anhui Agricultural Sciences;2006-24
3 WANG Ying~(1,2),ZHAO Hua-bin~1,HAO Jia-sheng~1(1. College of Life Sciences, Anhui Normal University, Wuhu 241000, China;2.Wuhu Hygienic School, Wuhu 241000, China);THEORY METHOD AND PERSPECTIVE OF MOLECULAR PHYLOGENETICS[J];Journal of Anhui Normal University(Natural Science);2005-01
4 CHENG Zhongping(Wuhan Institute of Botany, CAS, Wuhan 430074);Polymorphic Analysis of Crisp Peach Germplasm by Molecular Biology[J];Journal of Anhui Agricultural University;2003-02
5 NA Dong-chen,DENG Jun-min(Shanxi Normal University,Linfen,Shanxi 041004,China);The Improvement Methods of Extraction Total DNA in Ivy[J];Northern Horticulture;2008-09
6 Zhang Chunying (College of Landscape Architecture,Beijing For.Univ.,100083 ,P.R.China) Lin Tongxiang (Fujian Agriculture University ,Fuzhou 350002,P.R.China) Dai Silan Zhang Xiuying (College of Landscape Architecture,Beijing For.Univ.,100083 ,P.R.C;RAPDAnalysis on the Genetic Relationship of Peach Blossom Germplasm Resources[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1999-05
7 Cheng Zhongping; Chen Zhiwei;Hu Chungen;Deng Xiuxin.Wuhan Institute of Botany, Chinese Academy of Science, Wuhan 430074,P.R.China.;Analysis of Shouxingtao germplasm revealed by RAPD.[J];Journal of Beijing Forestry University;2002-03
8 Joni Kujansuu; Jing Tao; Ma Wanli; Luo Jüchun; Wang Guangfa;Sun Bo. School of Resources and Environment, Beijing For. Univ., 100083, P. R. China.;Ecological characteristics of Tilia amurensis in secondary broad leaved Korean pine forest community and in secondary mixed species forest community.[J];Journal of Beijing Forestry University;2003-01
9 Cheng Guangyou; Tang Xiaojie; Gao Hongbing; Shen Xihuan. Science College of Shantou University, Shantou, 515063, P.R.China.;Dormancy mechanism and relieving techniques of seeds of Taxus cuspidata Sieb.et Zucc[J];Journal of Beijing Forestry University;2004-01
10 FENG Xia-lian~1;HE Cheng-zhong~1;ZHANG Zhi-yi~1;AN Xin-min~1;YANG Kai~2;ZHANG You-hui~3.1 Key Laboratory for Genetic and Breeding in Forest Trees and Ornamental Plants, Ministry of Education,Beijing Forestry University,100083,P.R.China;2 Department of Biotechnology,Beijing Agricultural College,102206, P.R.China;3 Guanxian County Tree Nursery,Shandong Province,252528,P.R.China.;Establishment and optimization of an ISSR reaction system for Populus tomentosa Carr.[J];Journal of Beijing Forestry University;2006-03
【Secondary References】
Chinese Journal Full-text Database 5 Hits
1 Li Shaochen Gao Fuling Jiang Tingbo(Key Laboratory of Forest Tree Improvement and Biotechnology of Ministry of EducationNortheast Forestry University Harbin 150040);Analysis of Genetic Linkage Groups on Birch Using RAPD Markers[J];Scientia Silvae Sinicae;2008-05
2 SHI Jiang-Tao LI Jian(Key Laboratory of Bio-based Material Science and Technology,Ministry of Education,Northeast Forestry University,Harbin 150040);Purification and Identification of Populus ussuriensis Genomic DNA[J];Bulletin of Botanical Research;2009-02
3 YU Wen-Jing ZOU Chuan-Shan WANG Zhi-Ying* ZHANG Guo-Cai(College of Forestry,Northeast Forestry University,Harbin 150040);Populus nigra×Populus deltoids "108" Transformed the Chimeric Genes of Spider Insecticidal and Bt[J];Bulletin of Botanical Research;2009-05
4 ZENG Fan-Suo,WANG Xiao-Feng,LUO Wei,ZHAN Ya-Guang~* College of Life Science,Key Laboratory of Forest Tree Improvement and Biotechnology of Ministry of Education,Northeast Forestry University,Harbin 150040,China;Analysis of Pollen Vigor,Inheritance and Expression of Foreign Genes in Transgenic Birch(Betula platyphylla Suk.) Pollen[J];Plant Physiology Communications;2008-06
5 ZHANG Rui-Ping,LIU Gui-Feng,JIANG Jing,YANG Chuan-Ping,LI Cheng-Hao Key Laboratory of Forest Tree Genetic Improvement and Biotechnology of Ministry of Education,Northeast Forestry University,Harbin 150040,China;Establishment of Rapid Adventitious Bud Induction from Mature Zygotic Embryos of Birch(Betula platyphylla Suk.)[J];Plant Physiology Communications;2009-04
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Shuiming1,SONG Fengshun1,GONG Xuemei1,YANG Zhenjiang2,HUANG Yongfeng2,ZHU Liwu1(1.Department of Horticulture, Anhui Agricultural University,Hefei 230036;2.Administration Committee, Protection District of Dangshansu Pear Germplasm Resources);Isolation of Genomic DNA of Dangshansu Pearand Optimization of Its RAPD Analysis Condition[J];Journal of Anhui Agricultural University;2003-02
2 Zheng Jingsheng 1,2 Lü Bei 1 1 Hainan Provincial Key Laboratory of Crop Molecular Breeding,Sanya,572025 2 The Institute of Rice&wheat,Fujian Academy of Agricaltural Sciences,Fuzhou,350019;PCR Technique and its Practical Methods[J];Molecular Plant Breeding;2003-03
3 Yi Ganjun; Huo Heqiang; Cai Changhe; Qiu Yanping; Huang Ziran; Chen Dacheng(1 Pomology Research Institute, Guangdong Academy of Agric. Sci., Guangzhou, 510640;2 Dept. of Sericulture, South China Agric. Univ.;3 Dept. of Horticulture, South China Agric. Uni;DNA Isolation Procedure for AFLP Amplification Reaction of Litchi[J];JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY;1999-03
4 Zhu Heng Qu Feng Zhu Lihuang (Institute of Genetics, Academta Sinica, Beijing 100101);ISOLATION OF GENOMIC DNAS FROM FUNGI USING BENZYL CHLORIDE[J];Mycosystema;1994-01
5 Li Hong 1,2 Wang Xinli 1 1 Laboratory of Plant Biotechnology, Institute of Micobiology, Chinese Academy of Sciences, Bejing, 100080; 2 Department of Light Industry, Hebei Sciences and Technology University, Shijiazhuang, 050018;The Difficulties in the Isolation of RNA from Plant Tissues and their Resolving Strategies[J];BIOTECHNOLOGY INFORMATION;1999-01
6 WANG Zhuo-wei,YU Mao-de,LU Ceng(The Key Sericultural Laboratory of Agriculture Ministry,Southwest Agricultural University,Chongqing 400716,China);APPLICATION OF PVP IN TOTAL DNA EXTRACTION IN MULBERRY[J];Journal of Southwest Agricultural University;2001-01
7 LI Ming-Fang,ZHENG Xue-Qin(National Key Bio-technology Lab. For Tropical Crops,Chinese Academy of Tropical Agriculture Science,Haikou 571101,China);Development of SSR Markers in Litchi( Litchi chinensis)[J];Hereditas(Beijing);2004-06
8 DING Xiaodong 1,2 * ? ?&? ?LU Liuxin 1 ( 1 Deparment of Horticulture,Fujian Agricultural University , Fuzhou 350002) ( 2 Department of Horticulture,Northeast Agricultural University , Harbin 150030);STUDY ON GENOMIC DNA EXTRACTION FROM RECALCITRANT LITCHI[J];CHINESE JOURNAL OF APPLIED AND ENVIRONMENTAL BIOLOGY;2000-02
9 Dai Silan, ChenJunyu, Gao Rongfu, Ma Jiangsheng, and Li Wenbin (Beijing Forestry University , 100083; The Institute of Genetics, Academia Sinica, Beijing 100101);DNA Isolation Procedure for RAPD Amplification Reaction of Nine Dendranthema Species[J];Acta Horticulturae Sinica;1996-02
10 Jiang Changjun(Department of Tea Science,Anhui Agricultural University, Hefei)Chen Yan(Department of Biology, Anhui University, Hefei);A METHOD OF ISOLATING GENOMIC DNA FROM BRASSICA[J];;1995-04
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