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《Journal of Northeast Forestry University》 2006-04
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Systematic Classification of Some Species of Iris based on ITS Sequences

Wang Ling, Zhuo Lihuan(Northeast Forestry University, Harbin 150040, P. R. China)  
ITS sequences of ten kinds of Iris plants and an outgroup were obtained by primer design, PCR, gene cloning, sequencing and cluster analysis. The ITS1 section length is 219~243bp, 5.8S sequence length is 165bp in the species of Iris, and the ITS2 section length varies from 196bp to 239bp. The 5.8S sequence is too conservative, and the conservative sites occupy 81.9%. The ITS sequence has many advantages in the discrimination of the large classification grade such as Iris subgenus-joining. A more consistent systematic tree was obtained by cluster analysis with maximum parsimony method and neighbor method taking relative Belamcanda chinensis as an outgroup. The analysis indicates that the ITS sequence is more effective in establishing classification rank among genera. Considering the sequence of Chinese Iris species in Genebank, the result supports the taxonomy system of the upper group of Chinese Iris species, established by Zhao Yutang.
【CateGory Index】: Q949
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