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《Journal of Northeast Forestry University》 2007-09
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Cause and Restoration of Vitrification for Protocorm-like Body in Tissue Culture and Rapid Propagation of Phalaenopsis amabilis

Gu Defeng,Zhao Chunli,Zhang Hongwei(Jilin Agricultural University,Changchun 130118,P.R.China);Song Yanjun,Gu Sumin,Zhang Jiaxu(Jilin Guolian Agricultural Biotechnology Development Co.,Ltd.)  
An experiment was conducted to study the causes of vitrification and the reuse effect of protocorm-like body in cultivation of Phalaenopsis amabilis.Results show that the extent of vitrification for protocorm-like bodies aggravates with hormone concentration increasing and subculture time prolonging.The vitrification rates of two culture media with 5 mg·L-1 NAA and 10 mg·L-1 BA rose to 71% and 65%,respectively,when subculture to the ninth generation.The proliferation rate and regeneration rate of vitrification protocorm(2.2 and 183 individuals per bottle)were distinctly lower than those of formal protocorm(5.3 and 1 297 individuals per bottle).The vitrification was significantly reduced after 2 or 3 generations of cultivation using culture medium without hormone,and the number of vitrification protocorm could decrease to 0.84%.The differentiation ability of the restored protocorm and the state of plant growth were consistent with those of formal protocorm,and the restored protocorm could be continually applied to tissue culture.
【Fund】: 吉林省科技厅青年基金项目(20000556-2);; 吉林省科技厅重大项目(200504163)
【CateGory Index】: S682.31
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