Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Northeast Forestry University》 2007-09
Add to Favorite Get Latest Update

Cause and Restoration of Vitrification for Protocorm-like Body in Tissue Culture and Rapid Propagation of Phalaenopsis amabilis

Gu Defeng,Zhao Chunli,Zhang Hongwei(Jilin Agricultural University,Changchun 130118,P.R.China);Song Yanjun,Gu Sumin,Zhang Jiaxu(Jilin Guolian Agricultural Biotechnology Development Co.,Ltd.)  
An experiment was conducted to study the causes of vitrification and the reuse effect of protocorm-like body in cultivation of Phalaenopsis amabilis.Results show that the extent of vitrification for protocorm-like bodies aggravates with hormone concentration increasing and subculture time prolonging.The vitrification rates of two culture media with 5 mg·L-1 NAA and 10 mg·L-1 BA rose to 71% and 65%,respectively,when subculture to the ninth generation.The proliferation rate and regeneration rate of vitrification protocorm(2.2 and 183 individuals per bottle)were distinctly lower than those of formal protocorm(5.3 and 1 297 individuals per bottle).The vitrification was significantly reduced after 2 or 3 generations of cultivation using culture medium without hormone,and the number of vitrification protocorm could decrease to 0.84%.The differentiation ability of the restored protocorm and the state of plant growth were consistent with those of formal protocorm,and the restored protocorm could be continually applied to tissue culture.
【Fund】: 吉林省科技厅青年基金项目(20000556-2);; 吉林省科技厅重大项目(200504163)
【CateGory Index】: S682.31
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 5 Hits
1 Pan xue-feng,Wang an-shi,Li hai-zhu (College of life science and Agriculture,Hainan University,Haikou570228);A Reviewon The Progress of Phalaenopsis Rapid Propagation through Tissue Culture.[J];Tropical Forestry;2005-01
2 QIN Fan, ZHOU JiYuan (College of Life Sciences, Central China Normal University, Wuhan430079, China);Effect of Different Hormone on Rapid Propagation of Phalaenopsis[J];Journal of Wuhan Botanical Research;2003-05
3 Wang Huaiyiu ( The Floricultural Research Institute of Guangdong Academy of Agricultural Science, Guangzhou );RAPID CLONAL PROPAGATION OF PHALAENOPSIS BY TISSUE CULTURE[J];Acta Horticulturae Sinica;1989-01
4 Chen Zhilin,Ye Xiulin,and Liang Chengye (South China Institute of Botany,Academia Sinica,Guangzhou 510650,China);In Vitro Culture of the Inflorescence of Phalaenopsis[J];Acta Horticulturae Sinica;2003-02
5 Li Jun, Chai Xianghua, Zeng Baodang, Zhang Xiushan, Wang Xiyan, Zhan Haiyang, and Zhu Baoqing (Shantou Agricultural Science Research Institute, Shantou 515021,China);Mericlone Production Technical of Phalaenopsis[J];Acta Horticulturae Sinica;2004-03
Chinese Journal Full-text Database 10 Hits
1 WANG Yu-zhen~1, LIU Xiang-ling~(1,2), LUO Jing-lan~1(1.Center for Agricultural Resources Research, Shijiazhuang 050021;2. Graduate school of the Chinese Academy of Sciences, Beijing 100039);Study on the technology of tissue culture of fodder-type locust tree[J];Shandong Agricultural Sciences;2005-04
2 Lu Lingling1 Yi Kexian2 Xu Xuerong1 SunDequan1 Luo Ping1(1Southern Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Science,Zhanjiang,Guangdong,524091,China;2South China University of Tropical Agriculture,Danzhou,Hainan,571737,China);Study on Transplanting Techniques of Tissue Cultural Shoots of Agave Hybrid NO.11648[J];Anhui Agricultural Science Bulletin;2007-18
3 Han Yuanbin;Reproduction Technology of Photiniaxfrasery[J];Anhui Agricultural Science Bulletin;2008-11
4 Lei Cheng et al (Plant Tissue Culture Center Nanyang Agricultural School,Nanyang,Henan 473000);Study on Tissue Culture and Rapid Propagation Techniques of Gigas Type of Black Locust[J];Journal of Anhui Agricultural Sciences;2003-04
5 ZHANG Ming-li et al ( Department of Landscape, Beijing Forestry University, Beijing100083);Factors Resulting in the Brown from Cornus alb‘a Aurea’Culture in Vitro[J];Journal of Anhui Agricultural Sciences;2005-08
6 TANG Wei-bin et al (Department of Biology, Xingtai University, Xingtai, Hebei 054001);Study on Rapid Propagation and Tissue Culture of Sinningia speciosa[J];Journal of Anhui Agricultural Sciences;2005-08
7 ZHANG Li et al(Xuzhou Higher Vocational School of Bio-engineering,Xuzhou,Jiangsu 221006);Research Advances in Orchid Tissue Culture[J];Journal of Anhui Agricultural Sciences;2005-11
8 HAN Xiao-guang et al (Biology Department of Langfang Normal College,Langfang,Hebei 065000);Study on Multiple Shoot Induction and Plantlet Regeneration of Kale[J];Journal of Anhui Agricultural Sciences;2006-03
9 LI Dong-jie et al(Department of Biological Science and Engineering,Science and Technology University of Hebei,Shijiazhuang,Hebei 050018);Effect of Plant Growth Substance on Callus Growth and Browning of Rabdosia rubescens[J];Journal of Anhui Agricultural Sciences;2006-06
10 CHEN Shi-hua et al (College of Life Science,Shandong Normal University,Jinan,Shandong 250014);Optimized System of Tissue Culture,Rapid Propagation of Limonium sinense[J];Journal of Anhui Agricultural Sciences;2006-19
China Proceedings of conference Full-text Database 5 Hits
2 LIU Hai-shan LI Qing (College of Landscape Architecture,Beijing Forestry University,Beijing 100083);Studies on the Starting Culture Through the Clustered Shoots of Phalaenopsis spp.[A];[C];2007
3 YANG Li ZHANG Jia-yin GUO Chen HAN Jian DENG Zi-niu~* (College of Horticulture and Landscape,HNAU,Changsha 410128,China);A review of virus-free technique on Citrus[A];[C];2007
4 Wuang Xinwen;Chen Chuangguo Meizhou Reseach Institute of Agricultural Science, Meizhou, Guangdong 514071;Advance in Study on Tissue Culture and Rapid Propagation of Phalaenopsis[A];[C];2004
5 YAO Li-juan, CHEN Yi-zeng, XU Xiao-wei, LIN Shao-sheng, CHEN Zhong-lin, ZENG Ai-ping ( Zhejiang Subtropical Crops Institute, Wenzhou 325005, China );Experiment of Aseptic Seeding Technique of Cymbidium hybridum[A];[C];2006
Chinese Journal Full-text Database 10 Hits
1 CHANG Qing-yun et al(Key Laboratory for Genetic and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,Beijing Forestry University,Beijing 100083);Effects of Multi-Factor on Efficiency of Polyploid Induction with Colchicine[J];Journal of Anhui Agricultural Sciences;2007-31
2 Kang Xiangyang;Zhu Zhiti;Zhang Zhiyi. Journal of Beijing Forestry University (2000)22(3)1~4 [Ch,14 ref.]College of Resources and Environment,Beijing For.Univ.,100083,P.R.China.;Suitable period of high temperature treatment for 2n pollen of Populus tomentosa×P.bolleana.[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;2000-03
3 Li Yun;Tian Yanting;Luo Xiaofang(Research Centre of Improved Cultivar Breeding, BFU 100083);A Preliminary Study on the Mechanism of Vitrification of Mains zumi[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1996-01
4 Hao Guixia Zhu Zhen (Institute of Genetics, Chinese Academy of Sciences, Beijing, 100101, P. R. China) Zhu Zhiti (College of Forest Resources and Environment, Beijing For. Univ., 100083, P. R. China);Vitrification of Regeneration Shoots of P.tomentosa[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1999-01
5 HOU bo-xin, CHENG zheng-hong, LINfeng, Yu ge-fei, Yi ai-qing and Song qing-an(Hunan Academy of Forestry, Changsha410004);Select Breed and Popularize of New Variety Loropetalum Chinese Oliver var. rubrum[J];Journal of Chinese Urban Dorestry;2004-06
6 Lu Chengying,Tang Kehua,Tang hui,Huang Zaocheng,Zhong Wei (The Key Laboratory For Forest Products Chemical Industry Engineering of Hunan, Ji Shou University, Zhangjiajie, Hunan,427000);Priliminary study report of anti-virus activity of the pigment extracts of Loropetalum chinense var.rubrum Flower[J];Quarterly of Forest By-product and Speciality In China;2005-03
7 GUO Xiu-min,CHEN Duan-fen,FANG Zheng,GAO Yang University of Hebei, Baoding 071001, China);Study on the properties of anthocyanin from Impatiens hawkeri[J];Journal of Agricultural University of Hebei;2004-03
8 Zhao Jian(Institute of Materia Medica,Chinese Academca of Medicine Science,Beijing 100050)Yang Wenjie(College of Life Science,Northeast Normal University,Changchun 130024);RELATIONSHIPS OF OXYGEN FREE RADICALS STRESS AND OCCURRENCE OF VITRIFIED SHOOTS DURING MICROPROPAGATION OF PYRUS “PINGBO NO.1” (PYRUS PYRIFOLIA CULT.VAR.PINGBO NO.1) IN VITRO[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1998-02
9 DING Guo-chang~1,MA Zhong-li~1,LIN Si-zu~1,YOU Hua-ming,JI Ming,LAI Zhong-xiong~2 (1.College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China;2.College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China);Ultrastructure diversification of Acacia crassicarpa vitriform seedlings[J];Journal of Fujian College of Forestry;2007-01
10 Wu Ruojing; You Huamin;Beng Dongfei(Fujial College of Forestry, Napping 353001);Study on Vitrification of Regenerated shoots of Dianthus caryophyllus[J];JOURNAL OF FUJIAN COLLEGE OF FORESTRY;1995-04
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 WT4”BZ] YANG Mei chun, ZHOU Qi wei, XU Hong yuan, LU Mei ying ( Agricultural College, Guangxi University , Nanning 530005, China ) [WT5”HZ];Effect of external factors on protocorm like body inducement in Phalaenopsis leaves[J];GUIHAIA;2000-01
3 Zhang Xiuqing; Wang Zhiwu; Wang Chunying; Liu Yujing (Maize Research institute, Shandong Academy of Agricultural Sciences);THE STUDY ON PROTOCORN-LIKE BODY INDUCEMENT FROM ORCHID(PHALAENOPSIS SP.L.) SEEDING[J];JOURNAL OF LAIYANG AGRICULTURAL COLLEGE;1995-01
4 Liu Yongwei Mei Qingchao Cui Yanfang Mai Xiaoyan (Shenzhen Agricultural Science Research Centre);CLUSTER SHOOTS: A NEW WAY FOR RAPID VECETATIVE PROPAGATION OF PHALAENOPSIS[J];Chinese Journal of Tropical Crops;1993-02
5 Zhang Pifang Ni Dexiang Wang Fumin. and Wang Kaiji (Department Biology of Fudan University);STUDIES ON MORPHOGENESIS OF BULBLETS FROM BULB SCALES OF LILIUM DAVIDII VAR.UNICOLOR COTTON IN VITRO[J];Journal of Wuhan Botanical Research;1985-02
6 Zeng Songjun Peng Xiaoming Zhang Jingli Zhao Fengpan (South China Botanical Garden,The Chinese Academy of Sciences Guangzhou 510520);A STUDY ON TISSUE CULTURE AND RAPID PROPAGATION OF PHALAENOPSIS HYBRIDS[J];JOURNAL OF WUHAN BOTANICAL RESEARCH;2000-04
7 Wang Huaiyiu ( The Floricultural Research Institute of Guangdong Academy of Agricultural Science, Guangzhou );RAPID CLONAL PROPAGATION OF PHALAENOPSIS BY TISSUE CULTURE[J];Acta Horticulturae Sinica;1989-01
8 Guo Dachu, Liu Kebin, Chai Mingliang & Liu Feiyan (Hangzhou Botanical Garden, Hangzhou 310013, P.R.Ghina);Study on Rapid Clonal Propagation of Dendrobium, Cattleya and Phalaenopsis in Vitro[J];Acta Agriculturae Zhejiangensis;1991-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved