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《Journal of Northeast Forestry University》 2007-09
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Genetic Diversity of Harmonia axyridis from Maoershan Area Detected by ISSR Molecular Markers

Guan Huanan,Chi Defu,Yu Jia,Li Xiaocan(College of Life Sciences,Northeast Forestry University,Harbin 150040,P.R.China)  
The genomic polymorphism of 13 species of Harmonia axyridis(Pallas)with different splash types from Maoershan area was analysed using inter-simple sequence repeat(ISSR)markers.Thirteen among 40 ISSR primers were chosen for ISSR amplification.A total of 127 bands were generated,of which 120 bands were polymorphic bands(94.49%).Jaccard similarity coefficient analysed by NTSYSpc2.10e software showed that the genetic similarity coefficient was from 0.36 to 0.60 and the average genetic similarity coefficient was 0.51 according to the result of ISSR amplification.The clustering dendrogram was constructed by UPGMA method,and thirteen samples were divided into four major groups at a similarity coefficient of 0.50.Principal component analysis indicated that three splash types(H.axyridis var.spectabilis Fald,H.axyridis ab.34-quattuorsignata(Hem.)Yuan.,H.axyridis.ab.succinea Hope)were the masterstroke of heredity aberrance in H.axyridis(Pallas).ISSR analysis exhibits a high genetic diversity of H.axyridis(Pallas).Moreover,the variance of the vagina wing impression in H.axyridis was also analysed by the clustering result.
【Fund】: 黑龙江省森林重大病虫害高效安全持续控制技术(GB06B304);; 黑龙江省杰出青年基金“杨树人工林蛀干害虫化学生态控制技术”
【CateGory Index】: S718.7
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