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《Journal of Northeast Forestry University》 2008-01
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A Modification Method of Extracting Total RNA in Compositae Different Tissues

Liu Ying,Wang Li,Jiang Yang,Li Sibin,Qi Changqing,Zu Yuangang(Key Laboratory of Forest Plant Ecology of Ministry of Education,Northeast Forestry University,Harbin 150040,P.R.China)  
The total RNA was extracted from root,stem,leaf and bud of Mikania micrantha Kunth,Eupatorium adenophorum Spreng,Ambrosia artemisiifolia Linn,Eupatorium odoratum Linn,Iva xanthifolia Nutt by using modified guanidine hydrochloride method in order to construct a high performance method for isolating RNA from plant tissues.The amount and purity of the isolated total RNA were detected by spectrophotometer,and quality and integrality of RNA by agarose electrophoresis.Result shows that a higher amount of total RNA could be obtained from different plant tissues.The absorbance ratios(OD260nm/OD280nm) of the total RNA ranged between 1.8 and 2.0.After agarose electrophoresis,two bands representing 18S and 28S rRNA were clearly observed.The intensity of the 28S band was approximately twice the intensity of the 18S rRNA band.Therefore,total RNA with high quality and good integrality could be obtained from different plant tissues by using modified guanidine hydrochloride method.
【CateGory Index】: Q943.2
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