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《Journal of Northeast Forestry University》 2008-06
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Genetic Diversity of Elite Clones of Camellia oleifera by ISSR

Wang Baoming(Key Laboratory of Non-wood Forest Products of State Forestry Administration,Central South University of Forestry and Technology,Changsha 410004,P.R.China);Chen Yongzhong(Hunan Academy of Forestry);Tan Xiaofeng(Central South University of Forestry and Technology);Peng Shaofeng(Hunan Academy of Forestry);Shi Mingwang(Central South University of Forestry and Technology)  
The genomic DNA extracted from tender leaves of 23 elite clones of Camellia oleifera(Native species for edible oil in China)was used to perform ISSR(inter-simple sequence repeat)analysis.Of the 60 primers screened,10 pieces of DNA fingerprint profiles containing 102 bands with 70 distinct polymorphic bands(68.6%)were obtained,and each primer produced 3 to 10 bands with the band size ranging from 200 bp to 2 000 bp.Result indicated that Shannon's information index was 0.479 3,and the mean observed number of alleles and the mean effective number of alleles were 2.000 0 and 1.518 5,respectively.The genetic distance varied from 0.154 2 to 0.693 1.There exists abundant polymorphism among these elite clones of C.oleifera.The UPGMA analysis result shows that the 23 elite clones of C.oleifera could be classified into 4 groups,namely,18 elite clones(including Xianglin 156,Xianglin 210,Xianglin 4,etc)belong to the first group,Jinruan 2 and Xianglin 97 to the second group,Xianglin 34 and Xianglin 11 to the third group,Xianglin 81 to the fourth group when the genetic distance is 0.392.On the basis of these analyses,the relationship between the clustering result and the agricultural characters of C.oleifera was discussed,which provides some references for the identification of elite clones,phylogenetic analyses and the utilization of germplasm resources.
【Fund】: 国家林业局重点项目(200401);; 国家科技部农业科技成果转化基金(05EFN214300199);; “十一五”国家科技支撑计划专题(2006BAD18B02)
【CateGory Index】: S794.4
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