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《Journal of Northeast Forestry University》 2008-09
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Screening Experiment of Xylanase-producing Paenibacillus sp. and Optimization of Production Culture Conditions

Bao Yihong, Li Xuelong, Yang Chuanping(School of Forestry, Northeast Forestry University, Harbin 150040, P. R. China)  
A high xylanase-producing bacterium was chosen from 115 xylanase-producing bacteria isolated from the soil-samples which full of high hemicellulose and was identified as Paenibacillus sp. After single factor tests and orthogonal array, the best culture medium for strain produce xylanase was obtained as corncob xylan (carbon source) 30.0 g/L, tryptone (nitrogen source) 5.0 g/L, K2HPO4 5.0 g/L, and Tween 80 3.0 g/L. The optimal culture conditions were obtained as the initial pH of medium 7.0, temperature 32 degrees C, and rotation rate 220 r/min. The xylanase activity for the fermentation fluid reached 194.67 IU when cultured in the best culture medium and under the optimal culture conditions for about 96 hours, which was 1.58 times of the original conditions.
【Fund】: 黑龙江省青年基金(010-413591);; 东北林业大学杰出青年基金;; 国家博士后流动基金
【CateGory Index】: TQ925
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