Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Northeast Forestry University》 2008-12
Add to Favorite Get Latest Update

Optimization for ISSR-PCR Reaction System of Harmonia axyridis Using Orthogonal Design

Guan Huanan,Chi Defu,Zheng Yi,Yu Jia,Li Xiaocan(College of Life Sciences,Northeast Forestry University,Harbin 150040,P.R.China)  
Five factors of ISSR-PCR amplification system,Taq DNA polymerase,Mg2+,DNA template,dNTPs and primer,were optimized at four levels by the orthogonal design using Harmonia axyridis(Pallas) as the experimental material.Results of PCR were analyzed by DPS software.The optimal ISSR-PCR system for H.axyridis was obtained as DNA template 150ng,primer 0.5μmol/L,dNTPs 0.15mmol/L,Taq DNA polymerase 1.5U,and Mg2+ 1.25mmol/L in a 20μL reaction system.The suitable annealing temperature for the ISSR-PCR reaction was 53.3 degrees C according to gradient PCR.
【CateGory Index】: S476
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 Bai Jinjun Wei Anzhi Wang Jia Zhang Yazhuan Life Science College,Northwest A & F University,Yangling,712100;Optimization of an ISSR Analysis System of Apricot of Kernel-Consuming Based on Orthogonal Design[J];Molecular Plant Breeding;2009-06
【Citations】
Chinese Journal Full-text Database 6 Hits
1 WANG Yan-hua~(1,2),HOU Xi-lin~(1,*),XU Ming-yu~1gricultural University,Nanjing 210095,China;2 College of Horticulture, Hebei Agricultural University, Baoding 071001,China);Study on optimization for ISSR reaction system in Brassica campestris ssp.chinensis using orthogonal design[J];Acta Botanica Boreali-occidentalia Sinica;2004-05
2 Xie Yunhai1* Xia De'an1 Jiang Jing1 Lin Ping2 1 Department School of Forest, Northeast Forestry University, Harbin, 150040; 2 Institute of Heilongjiang Forestry Research, Harbin, 150040;Optimization for ISSR-PCR System of Fraxinus mandshurica Rupr.Using Orthogonal Design[J];Molecular Plant Breeding;2005-03
3 He Zhengwen Liu Yunsheng Chen Lihua Cao Meihong(Department of Neurosurgery, Xiangya Hospital, Hunan Medical University, Changsha 410008)Xia Jiahui(The State Key Lab. of Medical Genetics of China, Changsha 410078);Orthogonal design-direct analysis for PCR optimization[J];BULLETIN OF HUNAN MEDICAL UNIVERSITY;1998-04
4 XIAO Yang TANG Li-Hua BIAN Yin-Bing (Institute of Applied Mycology, Huazhong Agricultural University, Wuhan 430070);Preliminary application of degenerate primers in study on the mating factor of Auricularia auricula[J];Mycosystema;2006-04
5 Mu Liqiang Liu Yingnan Feng Fujuan Yang Guoting(Northeast Forestry University Harbin 150040);Establishment and Optimization of ISSR-PCR Reaction System for Tilia amurensis[J];Scientia Silvae Sinicae;2006-06
6 QI Jian-min1, ZHOU Dong-xin2, WU Wei-ren1, LIN Li-hui1, FANG Ping-ping1, WU Jian-mei1 (1College of Life Science / College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002; 2 Longyan Tabacco Branch Company, Longyan 364000 );A Comparison Between RAPD and ISSR Technology in Detection of Genetic Diversity of Jute[J];Scientia Agricultura Sinica;2004-12
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Luo Haiyan et al.(1 Tropical Crops Genetic Resources Institutes,Chinese Academy of Tropical Agriculture Sciences,Hainan 571737,China;2 Chinese Academy of Tropical Agriculture Sciences,Hainan 571737,China);ISSR Molecular Marker and its Using[J];Anhui Agricultural Science Bulletin;2008-19
2 YI Dao-sheng et al(Huo Ying-dong College of Bioengineering,Shaoguan College,Shaoguan,Guangdong 512005);Multiplex PCR Detection and Typing of Porcine Circovirus[J];Journal of Anhui Agricultural Sciences;2007-32
3 ZHANG Tao et al(College of Life Science and Technology,University of Electronic Science and Technology,Chengdu,Sichuan 610054);Optimization for ISSR Reaction System of Hordeum vulgare subsp.Vulgare with Orthogonal Design[J];Journal of Anhui Agricultural Sciences;2007-32
4 LIU Su-xia et al(Henan Normal University,Xinxiang,Henan 453007);Study on Optimization for RAPD Reaction System and Genetic Relationship in Punica granatum[J];Journal of Anhui Agricultural Sciences;2007-36
5 HUANG Zi-feng et al(Dongguan Institute of Agricultural Seeds in Guangdong Province,Dongguan,Guangdong 523063);Optimization of RAPD Reaction System for Lilium formolongi[J];Journal of Anhui Agricultural Sciences;2008-18
6 YU Jian-feng et al(Department of Biology and Food Engineering,Changshu Institute of Technology,Changshu,Jiangsu 215500);Establishment and Optimization of ISSR-PCR System in Trachidermus fasciatus Heckel[J];Journal of Anhui Agricultural Sciences;2008-33
7 YI Qiong et al(Biotechnology Research Center,Kunming University of Science and Technolgy,Kunming,Yunnan 650224);Analysis on Genetic Relationships among 8 Main Pear Cultivars in Yunnan Province Based on ISSR Molecular Markers[J];Journal of Anhui Agricultural Sciences;2009-02
8 HUO Guang et al(Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha,Hunan 410205);Genetic Diversity Analysis of 44 Shares of Hibiscus cannabinus L.Germplasm Resources Using ISSR Molecular Marker[J];Journal of Anhui Agricultural Sciences;2009-21
9 ZHANG Qi-feng et al(Heilongjiang August First Land Reclamation University,Daqing,Heilongjiang 163319);SSR-PCR Reaction System for Magnaporthe oryzae Optimized by Orthogonal Design[J];Journal of Anhui Agricultural Sciences;2009-22
10 ;Optimization of SSR-PCR reaction system in fungus by orthogonal design[J];Journal of Jilin University(Medicine Edition);2006-06
China Proceedings of conference Full-text Database 6 Hits
1 HOU Yu-jia, HE Qiao, LI Pin-wu, LIANG Guo-lu, PENG Ping, DENG Min (Chongqing Tea Researcfh Institute, Yongchuan, Chongqing 402160. China;Southwest University, Chongqing 400716, China;Sichuan Agricultural University, Yaan, Sichuan 625014, China);Genetic Polymorphism Analysis of Sichuan-Chongqing Tea Camellias Germplasm by Issr Molecular Marker[A];[C];2006
2 CHEN Rong-bing LIN Zheng-he CHEN Chang-song YOU Xiao-mei (Tea Research Institute,Fujian Acaedemy of Agricultrual Sciences, Fu’an Fujian 355014,China);Assessment of Genetic Diversity of Tea Detected by RAPD and ISSR[A];[C];2007
3 MA Qingfang,ZHANG Jiechi,ZHANG Peiqi,DAI Xiaodong,KONG Xianghui,HAN Zenghua,WANG Yuwen (Institute of Applied Microbiology HeiLongJiang of Academy of Science,Haerbin,150010);STUDY ON THE IDENTIFICATION OF CULTIVATED STRAINS OF AURICULARIA AURICULA BY ISSR MARKER[A];[C];2006
4 Pian Rui-qi (Laboratory for Biotechnology in Tropical and Subtropical Forest Science; College of forestry; South China Agricultural University Guangzhou 510642; Biological Institute, Beijing Forestry University Beijing 100083) Ni Hui-qun Chen Xiao-yang (Biological Institute, Beijing Forestry University Beijing 100083);Optimization of SRAP System and Genetic Relationship Analysis of Lespedeza[A];[C];2009
5 ;Optimization for ISSR Reaction System of jujube tree by Orthogonal Design[A];[C];2009
6 ZHENG Yi-qi~(1,2) WANG Zhi-yong~2 GUO Hai-lin~2 XUE Dan-dan~2 LIU Jian-xiu~(2*) (1.Department of Horticulture Nanjing Agricultural University Nanjing 210095 China; 2.Institute of Botany Jiangsu Province and Chinese Academy of Sciences Nanjing 210014 China);Optimization of SRAP-PCR System on centipedegrass Using Orthogonal Design and Selection of Primers[A];[C];2007
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Yan-hua~(1,2),HOU Xi-lin~(1,*),XU Ming-yu~1gricultural University,Nanjing 210095,China;2 College of Horticulture, Hebei Agricultural University, Baoding 071001,China);Study on optimization for ISSR reaction system in Brassica campestris ssp.chinensis using orthogonal design[J];Acta Botanica Boreali-occidentalia Sinica;2004-05
2 Chen Xuesen,Li Xianli,Zhang Yanmin,Wu Shujing, Shen Hongbo, and Shu Huairui College of Horticulture Shandong Agricultural University Taian Shandong 271018;Advances in Apricot Germplasm Resources Evaluation and Genetic Breeding[J];Journal of Fruit Science;2001-03
3 Xuan Jiping,Zhang Zhen ,Fang Jinggui ,Gao Zhihong,and Che Shengli (College of Horticulture,Nanjing Agricutural University,Nanjing,Jiangsu210095China);Construction of Apple Ccultivar IS SR Fingerprinting[J];Journal of Fruit Science;2002-06
4 QiaoYushan, Zhang Zhen, Fang Jinggui, ShenZhijun, and Zhao Mizhen (College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095 China; Research Institute of Horticulture, JiangsuA-cademy of Agricultural Sciences, Nanjing, Jiangsu 210014 China);Establishment of the Inter-simple Sequence Repeats Reaction System in Plum Germplasm[J];Journal of Fruit Science;2003-04
5 LIU Wei-sheng1,2, FENG Chen-jing3,YANG Jian-min3,LIU Dong-cheng4, ZHANG Ai-min4, LI Shaohua5*(1College of Agronomy and Biotechnology,China Agricultural University,Beijing 100094 China; 2Liaoning Institute of Pomology, Xiongyue, Liaoning 115009 China; 3Agricultural University of Hebei, Baoding, Hebei 071001 China; 4Institute of Genetic and Developmental Biology,Chinese Academy of Science,Beijing 100101 China;5Institute of Botany,Chinese Academy of Science, Beijing 100093 China);Optimization of ISSR reaction system and construction of cultivar fingerprint in apricot[J];Journal of Fruit Science;2005-06
6 WANG Jin1,HE Qiao2,OU Yi1,LIANG Guo-lu2,HE Bo2,GUO Qi-gao2,XIANG Su-qiong2(1Fruit Research Institute,Chongqing Academy of Agricultural Sciences,Jiangjin,Chongqing 402260 China;2College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400716 China);Germplasm identification and phylogenetic analysis of Plum using ISSR marker[J];Journal of Fruit Science;2008-02
7 He Zhengwen Liu Yunsheng Chen Lihua Cao Meihong(Department of Neurosurgery, Xiangya Hospital, Hunan Medical University, Changsha 410008)Xia Jiahui(The State Key Lab. of Medical Genetics of China, Changsha 410078);Orthogonal design-direct analysis for PCR optimization[J];BULLETIN OF HUNAN MEDICAL UNIVERSITY;1998-04
8 LUO Shu-ping1,ZENG Bin2,SUN Jin-ke1,LI Jiang2(1.Life and Science College;2.Horticultural College,Xinjiang Agricultural University,Urumqi 830052,Xinjiang,China);Optimization of ISSR-PCR Reaction System in Amygdalus Ledebouriana Schleche[J];Nonwood Forest Research;2007-03
9 Guo Lingfei 1,2 Zou Minghong1 Zeng Hui1 Du Liqing1 Lu Chaozhong1(1.South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences Zhanjiang 524091;2.College of Horticulture,South China University of Tropical Agriculture Danzhou 571737);Establishment and Optimization of ISSR-PCR System in Macadamia[J];Scientia Silvae Sinicae;2008-05
10 Qiu Yingxiong Fu Chengxin Kong Hanghui ( College of Life Sciences, Zhejiang University, Hangzhou 310029, China);Inter-simple Sequence Repeat(ISSR) Analysis of Different Cultivars in Myrica rubra[J];Journal of Agricultural Biotechnology;2002-04
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Yan-hua~(1,2),HOU Xi-lin~(1,*),XU Ming-yu~1gricultural University,Nanjing 210095,China;2 College of Horticulture, Hebei Agricultural University, Baoding 071001,China);Study on optimization for ISSR reaction system in Brassica campestris ssp.chinensis using orthogonal design[J];Acta Botanica Boreali-occidentalia Sinica;2004-05
2 ZHOU Dong xin, QI Jian min,WU Wei ren, LI Wei ming (College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Studies on DNA extraction and establishment of RAPD reaction system in jute[J];Journal of Fujian Agricultural University(Natural Science);2001-03
3 Xie Yunhai1* Xia De'an1 Jiang Jing1 Lin Ping2 1 Department School of Forest, Northeast Forestry University, Harbin, 150040; 2 Institute of Heilongjiang Forestry Research, Harbin, 150040;Optimization for ISSR-PCR System of Fraxinus mandshurica Rupr.Using Orthogonal Design[J];Molecular Plant Breeding;2005-03
4 Wang Jun and He Puchao ( Institute of Spend Wild Economic Animal and Plant, GAAS, Jilin 132109; Department of Horticulture, Northwestern Agricultural University);Extraction of Genomic DNA from Vitis amurensis and Its RAPD Analysis[J];JOURNAL OF FRUIT SCIENCE;2000-02
5 Zhang Qinglin and Luo Zhengrong  (College of Horticulture and Forestry,Huazhong Agricultural University,Wu han,Hubei430070China);ISSR Technology and Its Applications in Fruit Trees[J];Journal of Fruit Science;2004-01
6 He Zhengwen Liu Yunsheng Chen Lihua Cao Meihong(Department of Neurosurgery, Xiangya Hospital, Hunan Medical University, Changsha 410008)Xia Jiahui(The State Key Lab. of Medical Genetics of China, Changsha 410078);Orthogonal design-direct analysis for PCR optimization[J];BULLETIN OF HUNAN MEDICAL UNIVERSITY;1998-04
7 Du Fengguo Su Chunhua Li Yunfeng Qian Shulun(Forest Department of Jiln Forestry college)Zhang Xiaojiang Chen Ying Ll Guangcheng (Forest Mannagement Section of Hongshi Forestry Bureau);Study on the Seeds Anatomy Structure of Tilia amurensis Rupr,and Tilia mandshuricaRupr,et Maxim.[J];JOURNAL OF JILIN FORESTY UNIVERSITY;1994-02
8 WU Kang-Yun BIAN Yin-Bing ( Huazhong Agricultural University, Wuhan 430070);IDENTIFICATING TECHNIQUE OF INTRASPECIFIC HYBRIDS IN AURICULARIA AURICULA[J];Mycosystema;2002-02
9 Pang Hong Tang Cai Li Liying(Guangdong Entomological Institute, Guangzhou, 5060) ( , South China Agricultural University, Guangzhou, 5064);THE TECHNOLOGY FOR PRESERVATION OF CRYPTOLAEMUS MONTROUZIERI ADULT WITH THE ARTIFICIAL DIET[J];Natural Enemies of Insects;1996-02
10 He Yuqing; Zhang Yu; Sun Mei; He Guangchun; Wang Songwen; Zhu Yingguo ( College of Life Science, Wuhan University, Wuhan 430072; The Department of Zoology, Hong Kong University; The Department of Agronomy, Tianin Agricultural College, Tianjin 3003ll );Studies on the Relationship between Cultivated and Wild Rice Using ISSR Markers[J];Journal of Agricultural Biotechnology;2001-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved