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《Journal of Northeast Forestry University》 2009-09
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Construction of Eukaryotic Expression Vector of Laccase Gene (lcc1) from Coriolus versicolor under the Control of Lentinus edodes GPD Promoter

Deng Xun(Heilongjiang Forest Protection Institute,Harbin 150040,P.R.China);Song Ruiqing(Northeast Forestry University);Song Xiaoshuang(Heilongjiang Forest Protection Institute);Min Kai,Sun Haizhen(Northeast Forestry University)  
A pair of primers was designed according to the reported cDNA sequence of laccase gene,and the total RNA was extracted from mycelium of Coriolus versicolor.An amplification product about 1 563 bp was obtained by RT-PCR reaction.Sequence analysis showed that the cloned cDNA shared a 96 percent homology in comparison with the reported data.Two express vectors,pC-XG1-YZ-Lac and pC-XG2-YZ-Lac,were constructed by restriction enzyme digestion and legation using pCAMBIA1 381 xb as initial vector,and in these two vectors,the laccase cDNA of C.versicolor was under the control of two L.edodes GPD promoters Lg1 and Lg2,respectively.These two vectors were transformed into Agrobacterium tumefaciens EHA105 by the freeze-thaw method.
【Fund】: 黑龙江省自然科学基金项目(C200620);; 博士后研究人员落户黑龙江科研启动基金项目;; 哈尔滨市优秀学科带头人基金项目
【CateGory Index】: S646
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【Citations】
Chinese Journal Full-text Database 9 Hits
1 WANG Pei wu 1, WANG Dong chang 2, LI Yu 1 (1.Center of Biotechnology, Jilin Agricultural University, Changchun, Jilin 130118,China; 2. Department of Plant Protection, Laiyang Agricultural College, Laiyang, Shandong 265200, China);Advances of Genetic Engineering in Edible Mushroom[J];Journal of Jilin Agricultural University;2001-03
2 WU Kun1,2 ZhU Xian-Feng2 ZHANG Shi-Min2 JIA Xin-Cheng2 MIN Hang1 (1College of Life Sciences, Zhejiang University, Hangzhou 310029; 2College of Bioengineering, Henan Agricultural University, Zhengzhou 450002);STUDIES ON FERMENTATION CONDITIONS FOR LACCASE PRODUCTION FROM CORIOLUS VERSICOLOR[J];Mycosystema;2001-02
3 GUOMei PUJun DU Lian-Xiang LUFu-Ping BAI Dong-Qing (Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300222; Department of Food Science, Tianjin Agriculture College, Tianjin 300384; Key Laboratory of Ecology and Aquaculture, Department of Fishery Science, Tianjin Agriculture College, Tianjin 300384);CLONING OF cDNA ENCODING LACCASE (Lccl) FROM CORIOLUS VERSICOLOR AND EXPRESSION OF THE GENE IN PICHIA METHANOLICA[J];;2005-02
4 GUO Li-Qiong~1 WANG Xiu-Xu~(1*) LIU Yong~1 SHU Wei~2 LIN Jun-Fang~(1,3**) (~1College of Food Sciences,South China Agricultural University; ~2Test & Experiment Centre,South China Agricultural University; ~3Institute of Biomass Energy Research,South China Agricultural University,Guangzhou 510640);Functional analysis of gpd-Le and ras-Le promoters from Lentinus edodes[J];Mycosystema;2007-02
5 Yangfan1,2 Liu Ruirui1,2 Lin Junfang 1,3 Guo Liqiong 1,3 (1 State Key Laboratory of Tropical Crops Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101 2 Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou 571101 3 Department of Biotechnology, College of Food Science, South China Agriculture University, Guangzhou 510640);Sequence Analysis and Cloning of gpd Promoter from Volvariella valvace[J];Chinese Journal of Tropical Crops;2004-04
6 LIU Shi-qiang~1,YANG Li-qing~1,GUO Li-qiong~(1,2), LIN Jun-fang~(1,2)se Academy of Tropical Agricultural Sciences, Haikou 571101,China; ~2Department of Biotechnology, College of Food Sciences, South China Agricultural University, Guangzhou 510640, China);Cloning and Sequence Analysis of ras Promoter Region from Volvariella volvacea[J];Acta Edulis Fungi;2004-01
7 Yu Xiao-ling~1,Lin Jun-fang~(1,2),Guo Li-qiong~(1,2),Wang Shu-chang~1(~1State Key Laboratory of Tropical Crop Biotechnology,The Chinese Academy ofTropical Agricultural Sciences and Institute of Tropical Biological Sciences,Haikou 571101,China;~2Department of Bioengineering,College of Food Sciences,South China Agricultural University,Guangzhou 510640,China);Cloning and Characterization of ras and gpd Promoters from Lentinus edodes[J];Acta Edulis Fungi;2005-03
8 ZHANG Yin-bo 1,2 JIANG Mu-lan2 HU Xiao-jia2 ZHANG Gui-min1 MA Li-xin 1* ( 1 Molecular Microbiology Lab Hubei University, Wuhan 430062,China)( 2 Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062,China);Expression of a laccase gene from Pleurotus ostreatus in Pichia pastoris and characterization of the recombinant enzyme[J];Acta Microbiologica Sinica;2005-04
9 ZHANG Min~1,2 ,XIAO Ya-zhong~2 ,YU Cong~2 ,GONG Wei-min~1* (1.School of Life Sciences,USTC,Hefei 230026,China) (2.School of Life Sciences,Anhui University,Hefei 230039,China);Expression of cDNA of Laccase From Panus Rudis in Pichia Pastoris[J];Journal of University of Science and Technology of China;2004-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 REN Xuan,JIA Le*,SONG Zhen,DENG Peng(College of Life Science,Shandong Agricultural University,Taian 271018,China);Screening for Strain with High Laccase Activity by Protoplast Electrofusion of Inactivated Parental Strains[J];Shandong Agricultural Sciences;2008-08
2 WANG Dan-mei et al (College of Biological Science and Technology,Shenyang Agriculture University,Shenyang,Liaoning 110161);Study on Fermentation Conditions of Pycnoporus Sanguineus[J];Journal of Anhui Agricultural Sciences;2008-16
3 DENG Xun et al (Forestry College,Northeast Forestry University,Harbin,Heilongjiang 150040);Clone of gpd Promoter from Agaricus blazei Murrill and Its Sequence Analysis[J];Journal of Anhui Agricultural Sciences;2009-03
4 ZHAO Min;YANG Qian;XIN Ying;ZHU Shiqi;MU Qingfeng. Department of Life Science and Engineering, Harbin Institute of Technology,150001,P.R.China.;Induction and production of laccase in Elfvingia applanata.[J];Journal of Beijing Forestry University;2004-04
5 Deng Kaiye~(1,2),Li Yu~1,Ren LuQuan~2 (1.Jilin Agricultural University,Changchun1301182.The Key Laboratory for Terrain Machine Bionics Engineering,Ministry of Education,Changchun,Jilin 130051);The research progress of fugus based enzyme products[J];Quarterly of Forest By-product and Speciality In China;2005-06
6 GUO Mei1,LU Fu-ping2,LIU Min-yao3,LI Tuo-ping1,LIANG Peng1,PU Jun2(1.Department of Food Science,Tianjin Agriculture College,Tianjin 300384,China;2.Key Laboratory of Industrial Microbiology,College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300222,China;3.Tianjin Nuoao Technology Company,Tianjin 300384,China);Studies on the degradation of 2-chlomphenol by laccase-secreting engineered strain[J];Journal of Agricultural University of Hebei;2009-02
7 GUO Mei1, LU Fu-ping2, LIU Min-yao3, LI Tuo-ping1, LIANG Peng1, PU Jun2(1.Department of Food Science,Tianjin Agriculture College,Tianjin 300384,China;2.Key Laboratory of Industrial Microbiology,College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300222,China;3.Tianjin Nuoao Technology Company,Tianjin 300384,China);Decolorization of Anthraquinone Dye by Laccase-secreting Engineered Strain[J];Environmental Science & Technology;2009-04
8 WU Kun1,2, MIN Hang1, ZHU Xian-feng3, JIA Xin-cheng2, ZHANG Shi-min2, YU Zi-niu4 (1. College of Life Sciences, Zhejiang University, Hangzhou 310029, China; 2. College of Bioengineering, Henan Agricultural University, Zhengzhou 450002, China; 3. College of Bioengineering, Henan University, Kaifeng 475001, China; 4. Agricultural Ministry Key Laboratory of Agro-Microbiology, Middle China Agricultural University, Wuhan 430070, China);Purification and Properties of Laccases from Coriolus versicolor[J];Journal of Chemical Engineering of Chinese Universities;2003-02
9 WANG Sui-lou~(1,2), WU Xiao-zong~1, DUAN Xu-chang~3,SUN Jun-she~2 (1.College of Food and Biology Engineering,Zhengzhou Institute Of Light Industry,Zhengzhou 450002;;2.College of Food Biotechnology,China Agricultural University,Beijing 100083;3.College of Food Science and Engineering,Northwest Sci-Tech University of Agriculture and Forestry,Yangling Shanxi 712100);Mutagenic effects of ultra high pressure on laccase producing strains[J];Industrial Microbiology;2006-02
10 YANG Pei-zhou1,2,3,GUO Li-qiong1,2,WANG Yi-hong1,2,LIN Jin-fang1,2 (1.Institute of Biomass Research;2.Department of Bioengineering, College of Food Sciences,South China Agriculture University,Guangzhou 510640; 3.School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009);Cloning and sequence analysis of gpd-Fv promoter from F. velutipes[J];Industrial Microbiology;2008-03
China Proceedings of conference Full-text Database 7 Hits
1 SONG An-dong,ZHANG Bai-liang,WU Kun (College of Biotechnology and Food Science, Henan Agricultural University, Zhengzhou 450002 China; Key Laboratory of Rural Renewable Energy,Ministry of Agriculture, Zhengzhou 450002 China);Production of Lignocellulolytic Enzymes and Rice Straw Biodegradation by Coriolus versicolor[A];[C];2005
2 SONG An-dong,WANG Ming-dao,Du Feng-guang,Yan De-ran (Life Science College ofHenan Agricultural University,Zhengzhou 450002,China; Key Laboratory of Rural Renewable Energy,Ministry ofAgriculture,Zhengzhou 450002,China; Henan Tianguan Group,Nanyang 473000,China);Study on the lignocellulolytic enzymes producing from Pleurotus ostreatus[A];[C];2005
3 WANG De-han ,XIANG Qian-bin , CHEN Guang-yin (South China Agricultural University, Guangzhou, Guangdong 510642, China);Research Progress of Ecological Higher Value Application of Spent Mushroom Compost~1[A];[C];2007
4 FU Jun-Sheng XIE Bao-Gui (Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002);SEQUENCE ANALYSIS AND CLONING OF GPD PROMOTER FROM VOLVARIELLA VOLVACEA[A];[C];2005
5 Zhang xiao-yu Wujian-guo (College of Life Science and Technology, Huazhong University of Science and Technology ,Wuhan,430074);THE DEVELOPMENT OF ENGINEERING RESEARCHES OF BIODEGRADING AND BIOCONVERSION BY WHITE ROT FUNGI[A];[C];2006
6 Xiao Mei-li Song Xiao-ya The Institute of Applied Mycology Huazhong Agricultural University ,Wuhan,430070;THE FUNCTIONAL GENE CLONING DEVELOPMENT OF MICROFUNGUS[A];[C];2006
7 YAO qiang~1,LIU Yan~2,REN Peng-fei~1,REN hai-xia~1,QU ling~1,LI jin~1,GONG zhi-yuan~(1*) (1.Soil and Fertilizer Institute Shan Dong Acdemy of Agricultral Scieneces,Ji Nan,250100;2.Qingdao Agricultural University,Qing Dao,266109);Advance of Molecular Biology Technology Research of Edible Fungus[A];[C];2008
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Xiao ning 1 , WANG Hao 1, CHEN Huo ying 2, SHEN Da leng 1 (1. Institue of Genetics, State Key Laboratory of Genetic Engineering, School of Life Sciences; Research Centre of Gene Diversity and Designed Agriculture, Fudan University, Shan;Cloning and Sequence Analysis of a Fragment with Promoter Activity in Dunaliella salina (Chlorophyceae)[J];Journal of Fudan University;2002-02
2 Zhang Zhiguang Li Dongping Fang Fang Guo Jianchun (Department of Biology, Hunan Normal University,Changsha,410081);Protoplast Technique of Filamentous Fungi(VII) ——Influence of culture condition on preparation and regeneration of protoplasts[J];JOURNAL OF NATURNAL SCIENCE OF HUNAN NORMAL UNIVERSITY;1998-01
3 CHU Cheng-cai, CHEN Shuai, SI Li\|zhen\;(Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China);3-dimensional control of transgene expression[J];JOURNAL OF HANGZHOU TEACHERS COLLEGE;2001-04
4 WU Kun1,2 ZhU Xian-Feng2 ZHANG Shi-Min2 JIA Xin-Cheng2 MIN Hang1 (1College of Life Sciences, Zhejiang University, Hangzhou 310029; 2College of Bioengineering, Henan Agricultural University, Zhengzhou 450002);STUDIES ON FERMENTATION CONDITIONS FOR LACCASE PRODUCTION FROM CORIOLUS VERSICOLOR[J];Mycosystema;2001-02
5 WU Kun 1,ZHANG Shi min 2,ZHU Xian feng 2 (College of Biological Engineering,Henan Agricultural University,Zhengzhou 450002,China);Recent research advances on the lignin biodegradation[J];Journal of Henan Agricultural University;2000-04
6 Duan Xuewu Pang Xuequn Zhang Zhaoqi ( Dept. of Hort., South China Agric. Univ., Guangzhou 510642; College of Biotechnology, South China Agric. Univ., Guangzhou 510642, China );Low-temperature Storage of Straw Mushroom and Its Physiological Changes[J];Chinese Journal of Tropical Crops;2000-04
7 Wang Fuming Gong Xiurong Gao Junhui Liu Rixin (Edible Fungi Research Institute,Shanghai Academy of Agricultural Sciences,shanghai 201106);THE TECHNIQUE OF KEEPING FRESHNESS OF STRAW MUSHROOM AND RELATED PHYSIOLOGICAL CHANGES.[J];Acta Agriculturae Shanghai;1992-03
8 Yan Keqin Zhu Baocheng Zhao Huiliang Li Qingyu Wang Qian (Department of Biology of Hebei University, Baoding 071002) (Electrostatics Institute of Helei University, Bacding 071002) (Microbology Institute of Hebei Province, Baoding 071051);Transformation of Pleurotus sapidus Protoplasts by Electroporation[J];Chinese Journal of Biotechnology;1996-01
9 Chao Ya peng Qian Shi jun (Enzymology Department of Institute of Microbiology,CAS Beijing 100080);Fungal Laccase and Its Applications[J];Progress In Biotechnology;2001-05
10 Chen Mingjie Wang Zaoyue He Dongmei Pan Yingjie (Laboratory of Genetics and Breeding of Mushroom, Agricultural Ministry of China;Edible Fungi Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106);A Study of V.volvacea Protein Composition Change after Low Temperature Treatment[J];Acta Edulis Fungi;1995-04
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