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《Journal of Northeast Forestry University》 2009-09
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Construction of Eukaryotic Expression Vector of Laccase Gene (lcc1) from Coriolus versicolor under the Control of Lentinus edodes GPD Promoter

Deng Xun(Heilongjiang Forest Protection Institute,Harbin 150040,P.R.China);Song Ruiqing(Northeast Forestry University);Song Xiaoshuang(Heilongjiang Forest Protection Institute);Min Kai,Sun Haizhen(Northeast Forestry University)  
A pair of primers was designed according to the reported cDNA sequence of laccase gene,and the total RNA was extracted from mycelium of Coriolus versicolor.An amplification product about 1 563 bp was obtained by RT-PCR reaction.Sequence analysis showed that the cloned cDNA shared a 96 percent homology in comparison with the reported data.Two express vectors,pC-XG1-YZ-Lac and pC-XG2-YZ-Lac,were constructed by restriction enzyme digestion and legation using pCAMBIA1 381 xb as initial vector,and in these two vectors,the laccase cDNA of C.versicolor was under the control of two L.edodes GPD promoters Lg1 and Lg2,respectively.These two vectors were transformed into Agrobacterium tumefaciens EHA105 by the freeze-thaw method.
【Fund】: 黑龙江省自然科学基金项目(C200620);; 博士后研究人员落户黑龙江科研启动基金项目;; 哈尔滨市优秀学科带头人基金项目
【CateGory Index】: S646
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