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《Journal of Northeast Forestry University》 2014-09
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Transformation of Trichosanthin Gene to Populus euramericana 108

Sun Tingting;Zou Li;Xu Jifei;Yu Yang;Northeast Forestry University;Inner Mongolia University;  
In order to obtain transgenic Populus euramericana with Trichosanthin( TCS) gene,a plant expression vector was constructed and TCS gene was transformed into P. euramericana. The plasmid pT1-3 containing TCS promoter and structural gene which was donated by Peking University was digested by SacⅠ and BamHⅠ,the vector pCambia1301 was digested by the same enzymes. The digested and purified TCS genes were cloned into plant expression vector pC-tPro-TCS. The pCtPro-TCS with TCS gene was successfully transferred into Agrobacterium tumefaciens LBA4404,adapting to Agrobacteriummediated to transform the leaf explants,and 18 resistant shoots were obtained with transformation frequency of 4.3%. The resistant shoots were identified by PCR and PCR-Southern analysis. TCS gene was transferred into P. euramericana 108.
【Fund】: 黑龙江省自然科学基金项目(C200910)
【CateGory Index】: S792.11
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