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《Journal of Northeast Normal University (Natural Science Edition)》 2001-01
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Determination of the reduced glutathione by fluorescence spectrophotometry

GUO Li ping 1, LIU Guo liang 1, ZHANG Zhuo yong 1, LIU Si dong 1, WANG Li ying 2 (1. Faculty of Chemistry, Northeast Normal University, Changchun 130024, China; 2. College of Fundamental Medicine, Norman Bethune University of Medical Sciences,  
An improved fluorescence spectrophotometric method was developed for the reduced glutathione(GSH) determination based on the reaction of GSH with orthophthaldehyde (OPA). Some affecting parameters including amount of OPA, stability of the fluorescence system, etc. were investigated and optimized. Linear dynamic relationship was obtained in the concentration range of 0.08~0.8 μg/mL for glutathione. The regression equation was as following: I=0.984 4+84.15c . The correlation coefficient was 0.999 9. The method was also applied to determine GSH in soybean extracts by electromagnetic field technique and satisfactory results were obtained.
【Fund】: 吉林省卫生厅科研基金
【CateGory Index】: O629;O657.3
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