Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Northeast Normal University (Natural Science Edition)》 2001-01
Add to Favorite Get Latest Update

Determination of the reduced glutathione by fluorescence spectrophotometry

GUO Li ping 1, LIU Guo liang 1, ZHANG Zhuo yong 1, LIU Si dong 1, WANG Li ying 2 (1. Faculty of Chemistry, Northeast Normal University, Changchun 130024, China; 2. College of Fundamental Medicine, Norman Bethune University of Medical Sciences,  
An improved fluorescence spectrophotometric method was developed for the reduced glutathione(GSH) determination based on the reaction of GSH with orthophthaldehyde (OPA). Some affecting parameters including amount of OPA, stability of the fluorescence system, etc. were investigated and optimized. Linear dynamic relationship was obtained in the concentration range of 0.08~0.8 μg/mL for glutathione. The regression equation was as following: I=0.984 4+84.15c . The correlation coefficient was 0.999 9. The method was also applied to determine GSH in soybean extracts by electromagnetic field technique and satisfactory results were obtained.
【Fund】: 吉林省卫生厅科研基金
【CateGory Index】: O629;O657.3
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 DING Yu~(1,2) MIAO Jin-Lai~3 LI Guang-You~3 JIAN Ji-Chang~(1,2) WU Zao-He~(1,2) (1.Fisheries College of Guangdong Ocean University,Zhanjiang,524025;2.Guangdong Provincial Key Lab of Pathogenic Biology and Epidemiology for Aquatic Economic Animals,Zhanjiang,524025;3.Key Lab of Marine Bioactive Substances,State Oceanic Administration,Qingdao,266061);THE OPTIMAL CONDITION FOR GSH PRODUCTION FROM ANTARCTIC ICE MICROALGAE BERKELEYA RUTILANS GJ01[J];Oceanologia Et Limnologia Sinica;2008-01
2 ZHENG Zhou1,MIAO Jin-lai1,CHEN Hao2,DIND Yu3(1.Key Laboratory of Marine Bio-active Substances,State Oceanic Administration,Qingdao 266061,China;2.State Key Lab Cultivation Base,Shandong Provincial Key Lab of Opthalmology,Qingdao 266071,China;3.Fisheries College,Guangdong Ocean University,Zhanjiang 524088,China);Primary study on glutathione and glutathione-related enzymes of Antarctic ice microalgae[J];Chinese Journal of Marine Drugs;2009-01
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Tang Aiguo Yang XinanCentral Laboratory. The Second Affiliated Hospital. Hunan Medical University;AN IMPROVED FLUOROMETRIC METHOD IN DETERMINATION OF PLASMA GLUTATHIONE[J];;1990-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Min-hang,LIU Guang-qing,LIN Si-zhong,SHEN Yue-wei, XU Qing-hua, LU Yong-jie (Institute of Naval Medical Research, Shanghai 200433, China);The Protection of Glutamine on Low-Dose Radiation in Rats[J];Amino Acids & Biotic Resources;2004-04
2 SHI Ying qin, ZHANG Wen jie, WANG Qing, ZHANG Xiao peng, ZHOU Jian li (Nutriology Center,Hebei Province Hospital,Shijiazhuang 050071,Hebei, China);Effect of alanyl-glutamine dipeptide on glutathione concentration and antioxidation in burned rats[J];Parenteral & Enteral Nutrition;2002-01
3 Cheng Xiaoxin;et al.(Department of Parasitology, Dalian Medical University);Change of oxygen free radical during trichinella spiralis infected in mice liver[J];JOURNAL OF DALIAN MEDICAL UNIVERSITY;1996-02
4 Wang Yukun; et al (Second Hospital Affiliated, Daltan Medical University);Endotoxemia and hepatic changes in acute peritonitis[J];JOURNAL OF DALIAN MEDICAL UNIVERSITY;1996-03
5 ;Clinical and Experimental Study on the Treatment of postoperative Vleus with Herds Fuan(Abstract)[J];JOURNAL OF DALIAN MEDICAL UNIVERSITY;1997-02
6 Lu Xuemei; Zhang Suhua(From the Department of Otolaryngology, Head and Neck Surgery, People's Hospital ofLiaocheng Prefecture, Shangdong Liaocheng 252000);A preliminary study on the course of treatment of hyperbaric oxygen therapy in patients with idiopathic sudden hearing loss[J];;1997-05
7 Tang Heping 1 Zhang Jiliang 2 Xiao Rong 2 Wen Haiquan 2 Su Yaping 2 (1 The College of Food Science & Technology, HNAU,Changsha,410128) (2 Dermatological Department of the Second Affiliated Hospital, HNMU, Changsha,410011);A STUDY OF THE MECHANISM AND FUNCTIONS OF THE TREATMENT TO SUPERFICIAL LESION PIG MENTATION OF RABBITS WITH CATECHINES[J];JOURNAL OF HUNAN AGRICULTURAL UNIVERSITY;1996-06
8 Wang Xincai Liu Ruifang Ji Lili et al(Luoyang Medical College,Luoyang 471003);Investigation on Pinworm Infection of Children in the Urban and Rural Kindergarten in Luoyang City[J];HENAN MEDICAL RESEARCH;1998-02
9 Liang Min, Hou Fanfan, Tian Jianwei et al . Nanfang Hospital, First Military Medical University, Guangzhou 510515, China;OXIDATIVE STRESS IN END STAGE RENAL DISEASE[J];Medical Journal of Chinese People's Liberation Army;2003-06
10 Lu Xuemei;Zhang Suhua (Department of Otolaryngology,People's Hospital,Liaocheng Prefecture, Shandong Province, 252000 );The dosage of hyperbaric oxygen therapy in patients with sudden hearing loss[J];JOURNAL OF CLINICAL OTORHINOLARYNGOLOGY;1997-09
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Zhang Buchang Zhao Zhiping Yuan Hualing Chen Zuzhi Bi Jianjun (Department of Biology Anhui University Hefei 230039);Study on Isolation, Purification and Glucopeptide Bond of Glucoprotein form Asparagus Cochinchinensis[J];JOURNAL OF ANHUI UNIVERSITY(NATURAL SCIENCES);1996-04
2 ZHAO Hua,DAI Yan,QI Gang (College of Food Science and Bioengineering,Tianjin University of Science and Technology,Tianjin 300222,China);Effect of UV injuring culture on UV absorption capability of yeast hydrolysate[J];China Surfactant Detergent & Cosmetics;2004-02
3 CHEN Jian~*, HE Zhong-yu, CHA Chuansin, LIU Huan (College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072,China);The Electrochemical Behavior and Determination of GSH at the Powder Microelectrode[J];Electrochemistry;2004-03
4 CHEN Kun-ming~(1,3), GONG Hai-jun~2, WANG Suo-min~(2*) (1 Department of Biology, Tianshui Normal College, Tianshui Gansu 741001,China; 2 School of Life Science, Lanzhou University, Lanzhou 730000,China; 3 Institute of Botany, Chinese Academy of Scinces, Beijing 100093,China);Glutathione metabolism and environmental stresses in plants[J];Acta Botanica Boreali-occidentalia Sinica;2004-06
5 Yang Zhimin;Yan Jingyi;Zheng Youfi and Zhang Suqing(Biological Science Division,Department of Applied Meteorology,Nanjing Institute of Meteorology,Nanjing 210044)Chen Shangqian and Lu Xufeng(Getang Agricultural Science and Technology Station in Nanjing,Na;EFFECTS OF ULTRAVIOLET RADIATION ON THE DEGRADATION OF WHEAT LEAF CHLOROPHYLL UNDER DIFFERENT CONDITIONS[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1995-04
6 Liu Yan (North China Electric Power University,Department of mathematics,Beijing 102206);ATMOSPHERIC OZONE LAYER,SOLAR UV RADIATION AND THE HEALTH OF MANKIND[J];PROGRESS IN GEOPHYSICS;1998-03
7 Liu Langxin;Cheng Zongdao ;Wang Guangci;Tian Ye;The Extraction Technique of the Cell Wall Polysaccharid es from Beer Yeast[J];JOURNAL OF CHONGQING UNIVERSITY(NATURAL SCIENCE EDITION);1994-06
8 Lin Li, Cao Xuni, Zhang Wen, Zhou Yuyan, Li Jinhua, Jin Litong * (Department of Chemistry, East China Normal University, Shanghai 200062);Application for Multiwall Carbon Nanotubes Modified Electrode to Liquid Chromatography for Determination of the Thiols in Human Whole Blood[J];Chinese Journal of Analytical Chemistry;2003-03
9 [WT5”BZ]Wang Qingjiang, Chen Xiang, Ding Fei, Lü Jin, He Pingang, Fang Yuzhi* (Department of Chemistry, East China Normal University, Shanghai 200062);Determination of Water-soluble Small Molecular Antioxidants by Capillary Electrophoresis with Electrochemical Detection[J];Chinese Journal of Analytical Chemistry;2005-07
10 LU Wei (Jiangsu Staff Medical University Nanjing,China,210029);The Chemical and Medical Functions of Glutathion[J];Journal of Chemical Education;2004-01
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 WANG Ling~(1),PU Wan-xia~(1),CHANG Hui-yun~(2),HUA Lan-ying~(1),SUOLANG Si-zhu~(3),HU Zhen-yin~(1),WEI Xiao-juan~(1),MENG Xiao-qin~(1) (1.Lanzhou Institute of Animal & Veterinary Pharmaceutics Sciences,CAAS,Lanzhou,Gansu,730050,China;2.Lanzhou Veterinary Research Institute,CAAS,Lanzhou,Gansu,730046,China;3.Department of Animal Science and Veterinary Medicine,Tibetan Agriculture and Husbandry University,Linzhi,Tibetan,860000,China);Effect Factors on Cultivating Saccharomyces cerevisiae[J];Progress in Veterinary Medicine;2006-11
2 BAI Ai-qin1,GUO Kun-liang2,QIN Guang-li1,CAO Wen-tao2,QIU Shu-yi2 and WANG Qiang2(1.Chemical Engineering College of Guizhou University,Guiyang,Guizhou 550003;2.Life Science and Technology College of Guizhou University,Guiyang,Guizhou 550002,China);Research on Stress Response of Alcohol to Produce Live Yeast Cell Derivatives[J];Liquor-Making Science & Technology;2008-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved