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《东北农业大学学报(英文版)》 2009-02
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Optimization of SRAP-PCR Reaction System in Flax

LI Dongmei, ZHOU Yadong, LENG Chao, SU Yu, and LI Ming College of Agriculture, Northeast Agricultural University, Harbin 150030, China  
Part of wild flax species and cultivated species were used as materials for optimizing SRAP-PCR reaction system, and concentration gradient test was made for all factors in order to select and establish the optimal program and system f itting for amplifying high diversity, good reproducible and clear bands. This program and system could well meet the requirement of flax SRAP and prove that SRAP marker was feasible for researching genetic polymorphism of flax.
【CateGory Index】: S563.2
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 GUO Da-long, LUO Zheng-rong*(Key Laboratory For Horticultural Plant Biology, MOE, Huazhong Agricultural University, Wuhan, Hubei 430070 China);Optimization of SRAP -PCR in some Diospyros spp.[J];Journal of Fruit Science;2006-01
2 LI Shuang-shuang,ZHAO Rui-hua,YAN Gui-qin,HAO Gang(Institute of Biodiversity,School of Life Science,Shanxi Normal University,Linfen 041004,Shanxi,China);Establishment & Optimization of SRAP Analysis System of Potato Genome[J];Journal of Shanxi Normal University(Natural Science Edition);2007-04
3 YUAN Ju-hong1,2, QUAN Jun-ping1, HU Mian-hao3, SUN Shi1, PENG Feng1, XIA Bing1,① (1. Institute of Botany, Jiangsu Province and the Chinese Academy of Sciences, Nanjing 210014, China; 2. Resource and Environment Management Institute, Jiangxi University of Finance and Economics, Nanchang 330032, China; 3. College of Agriculture and Biology,Shanghai Jiaotong University, Shanghai 201101, China),;Establishment and optimization of SRAP-PCR amplification system for Lycoris radiata[J];Journal of Plant Resources and Environment;2007-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Xiao-hui et al(Institute of Horticulture,Henan Academy of Agriculture sciences,Zhengzhou,Henan 450002);Optimization of SRAP-PCR Program and System in Citrullus lanatus[J];Journal of Anhui Agricultural Sciences;2007-27
2 XU Yingying,QU Shuping,CUI Chongshi(College of Horticulture,Northeast Agricultural University,Harbin 150030,China);Optimization of SRAP-PCR program and system in Chinese cabbage[J];Journal of Northeast Agricultural University;2008-08
3 WANG Xiao-hui1,TANG Xiao-chuang1,YANG En-xiu1,JIANG Cheng-xi1,LI Min2,LI Xiao-kun1,2(1.Ministry of Education,Engineer Research Center of Bioreactor and Pharmaceutical Development,Jilin Agricultural University,Changchun 130118,China;2.Pharmacy School,Wenzhou Medical College,Wenzhou,Zhejiang 325035,China);Optimization of RAPD Reaction systems for Curcuma wenyujin[J];Northern Horticulture;2008-04
4 Liu Lijun Meng Zuqing Xing Xiulong Peng Dingxiang* College of Plant Science &Technology, Huazhong Agricultural University, Wuhan, 430070;Optimization for SRAP Reaction System in Ramie (Boehmeria nivea L. Guad)[J];Molecular Plant Breeding;2006-05
5 GUO Da-long et al(College of Forestry,Henan University of Science and Technology,Luoyang,Henan 471003);Establishment and Optimization of SRAP Amplification System in Bitter Melon(Momordica charantial L.)[J];Journal of Anhui Agricultural Sciences;2008-18
6 LU Juan1,ZHANG Shao-ling1,LIU Qing-zhong2,WU Jun1,ZHANG Rui-ping1,LIAOYANG Wen-ke1,CHEN Ting1(1College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095 China;2Shandong Institute of Pomology,Tai'an,Shan-dong 271000 China);Optimization of SRAP-PCR system and its application in genetic diversity analysis of cherry[J];Journal of Fruit Science;2009-02
7 Zan Fenggang 1,2 Wu Zhuandi 1,2 Zeng Qi 1,2 Zhang Huiyun 2 Li Mingfang 1 Zheng Xueqin 1 1 Institute of Tropical Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101; 2 Hainan University, Haikou, 570228;Genetic Diversity Analysis of Litchi Germplasm by SRAP Markers[J];Molecular Plant Breeding;2009-03
8 ZHANG Jian-ping1,WANG Bin2,ZHAO Li-juan3,WANG Li-min1,YANG Chong-qing2,XIE Rui-xia2,DANG Zhan-hai1(1 Institute of Crop Science,Gansu Academy of Agricultural Science,Lanzhou 730070,China;2 Agronomy College,Gansu Agricultural University,Lanzhou 730070,China;3 Institute of Bio-technology,Gansu Academy of Agricultural Science,Lanzhou 730070,China);Screen of SSR Markers from EST of Flax(Linum usitatissimum L.)[J];Acta Botanica Boreali-Occidentalia Sinica;2009-05
9 Xiong Faqian 1,3 Tang Ronghua 2,3 Chen Zhongliang 3 Pan Linghua 3 Zhuang Weijian 4 1 College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002; 2 Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007; 3 Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning, 530007; 4 College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002;SCoT:A Novel Gene Targeted Marker Technique Based on the Translation Start Codon[J];Molecular Plant Breeding;2009-03
10 Wang Xuejun Liu Guojian Chang Ruifeng Han Jicheng Guo Encai Changli Institute of Polomogy, Hebei Academy of Agriculture and Forestry Sciences, Changli, 066600;Optimization of Annealing Temperature of SRAP-PCR in 5 Temperate Fruits[J];Genomics and Applied Biology;2009-03
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1 Jianming GAO,Zhongyou PEI,Qiuling CHEN,Jinzhao WEI,Zifang Li Feng LUO,Bo DING,Shoujun SUN* (Department of Agronomy,Tianjin Agricultural University,Tianjin 300384,P.R.China;;AFLP and SRAP fingerprinting of a collection of sweet sorghum germplasm[A];[C];2008
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 GUO Da-long, LUO Zheng-rong*(Key Laboratory For Horticultural Plant Biology, MOE, Huazhong Agricultural University, Wuhan, Hubei 430070 China);Optimization of SRAP -PCR in some Diospyros spp.[J];Journal of Fruit Science;2006-01
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9 Luo Zhengrong,Li Fafang,and Cai Lihong (Laboratory of Pomology,Department of Horticulture,Huazhong Agricultural University,Wuhan 430070);Molecular Systematics of China Native Nonastringent Persimmon Based on Random Amplified Polymorphic DNA[J];ACTA HORTICULTURAE SINICA;1999-05
10 Liang Jingxia1 Qi Jianmin1 Wu Weiren1 Zhou Dongxin2 Chen Shunhui3 Wang Tao1 Tao Aifen1 1. Fujian Agriculture and Forestry University,Fuzhou 350002 2. Longyan Branch of Institute of Fujian Tobacco Agricultural Science, Longyan 364000 3. Institute of Fujian Tobacco Agricultural Science, Fuzhou 350003;DNA extraction and the establishment of SRAP reaction system in tobacco[J];Acta Tabacaria Sinica;2005-04
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