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《Journal of Colorectal & Anal Surgery》 2009-03
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Establishment of animal models in colorectal cancer with liver metastasis

Yang Jianfeng,Zhang Sen,Gao Feng,et al.Department of the colorectal and anal surgery,the First Affiliated Hospital of Guangxi Medical University,Naning 530021,China  
Objective TO define a suitable method of establishing animal model of colorectal cancer with liver metastasis by comparing several methods.Methods BALB/c mouses were grouped randomly into 11 groups by doses of adenocarcinoma cells and methods of injection,that is 0.1 mL,0.2 mL,0.3 mL,1.0 mL(colon adenocarcinoma cells in 1×106/mL) by spleen,rectum,peritoneal cavity(no 1.0 mL dose group in peritoneal group).The rate of liver metastases were compared between the three groups and within each group.Results Three methods were able to replicate an animal model of colorectal cancer with liver metastases.The liver metastasis rates were 50.0%,77.2%,50.0%,27.0% respectively in 0.1 mL,0.2 mL,0.3 mL,1.0 mL doses by spleen;there were 50.0%,53.1%,16.7%,6.7% respectively in 0.1 mL,0.2 mL,0.3 mL,1.0 mL doses by rectum,and 10.0%,22.2%,10.0% in 0.1 mL,0.2 mL,0.3 mL doses through peritoneal cavity.Conclusion Inject through spleen with a dose of 0.2 mL(1×106/mL) is a effective way to establish animal model of colorectal cancer with liver metastasis.Inject through rectum with a dose of 0.1 mL or 0.2 mL(1×106/mL) CT26 is a simple way of establish animal model of colorectal cancer with liver metastasis,and follow the law of liver metastases of colorectal cancer also.
【Fund】: 国家自然科学基金项目(No.30560150 30672059);; 广西医疗卫生重点科研课题项目(No.200404)
【CateGory Index】: R735.3;R-332
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