PRELIMANARY STUDIES ON THE SCREENING OF NaCI-TOLERANTE SOYBEAN PLANTLET REGENERATED FROM TISSUE CULTURE
Chen Yunzhao(Shanxi Agricultural University)
The explant of young true leaves of soybean were cultured in the media containing NaCl. The result showed that the rate of callus induction was decreased as the Nacl concentration was increasing. In the media of high NaCl concentration was in creasing. In the media of high NaCl concentration the pullulation rate of callus was slower, the volume smaller. However, the structure because closer, texture harder. Differentiation frequency of the regenerated plantlet was decreased as the NaCl concentration was increasing. The maximum NaCl concentration in which regenerated plantlet could be obtained was 0.25%. Comparing with the control regenerated plantlets, they had short plant, thick stem and thick leaf which seemed consistently to be with succulence of halophyte. The small number of callus obtained in media containing NaCl 0.3% only had differentiation of bud. But by transfer the callus tissues gradually from lower NaCl concentration media to higher concentration regenerated plantlet could differentiate out in the same selection media (NaCl 0.3%). The frequency of differentiation is 2.5%. Small number of callus in media containing more NaCl (0.35%) could differentiate bud as well. It indicates that the maximum NaCl concentration of screening NaCl-tolerante regenerated plantlet can increase to 0.3% then it is passible to reach to 0.35% by constantly enhancing selection pressure.