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《Soybean Science》 1991-01
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STUDY OF EXOGENOUS DNA DIRECTLY TRANSFERED INTO SOYBEAN

Lei Bojun Yin Guangchu Lu Caihua Qian Hua Zhang Kaiwang Zhou Sijun Wang Shulin(Soybean Research Institute HAAS Harbin China)  
This paper reports the methods and results of Exogenous DNA directly transfered into culti-var soybeans through formed passage of the soybean pollen tube after self-polination.Throug studies on the different transfering stage and methods of 517 flowers of 10 groups of soybean. Results show that the survival rate is 31%; the plant forming rate is 58%; the transformed rate is 8% that cutting the stigma off and putting DNA extraction on the cut after soybean self-pollinating 6-32 hours. By ejecting the ovary after soybean self-pollination the survival rate is 1. 3%. Plant forming rate is 0. The transformed off spring shows variations in mature stage, plant type, flower colour, skin colour of seed, hundred-seed weight and protein content of soybean. The single-plants or plant-lines of D2 were analysed with isoenzyme, the bands and activity of D 2 peroxidose isoenzyme show significant diffirence. Both plants of D8705-2-9 and D8705- 2-10 with significant variation in D1 occured "Frenzeid separation". Their enzyme bands show unregulay. The D2 plant-line enzyme activity of D8701 whose protein content is higher than the receipt out the phenotype has no clear variation shows significant increasing and mainly concentrates on A1 and B zone.The results indicate that, directly transfering foreign DNA into host plant egg cell, Zygote or early stage embryo, some segments can be inserted into host plant DNA and can espress. The results also indicate that it is possible to improve soybean quality by using the pollen tube passage to transfer foreign DNA into soybean.
【Fund】: 国家自然科学基金
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