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《Soybean Science》 1996-04
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STUDY ON TRANSFERRING SOYBEANS MOSAIC VRIOUS COATPROTEIN (SMV-CP) GENE INTO SOYBEANS BY RI-PLASMID

Xu Xiangling Li Xinghua Liu Weihua Li Jilin(Harbin Normal University 150080)Wang Yi(JiLin Agricultural University 130043)  
The PES plasmid (T-DNA carrying SMV-CP gene) in the E. coli JM109 and was intro-duced into Agrobacterium rhizogenes (PRiA4b) with Ri-plasmid by tri-perental mating and wastranferred into cuiltived soybeans Hefen25, Heinong33 et al. by means of binary vecotors. Thehairy roots were inducted from cotyledonary node. Hypocotyl and whole plants by in Jection ofbacteria transferred. The analysis of paper electrophpresis showed that alkaliod found in the 25%hairy roots and 30% regenerated plants. The cullus of formation of hairy roots were inductedform hypocotylars. The cullus butting, adventition, leaf-like and embryoid were obtained fromimmature embryos and adventitiou were produced form organogeninsis. PCR and southern blot-ting proved that the SMV-CP gene have been introduced and interjected into genome of soybeans.
【Fund】: 本课题为黑龙江省科委资助重点攻关课题
【CateGory Index】: S435.65
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